GPMDB00245OtherMohien U, et al.InstrumentNot AvailableNot availableMosquitordinglas@jhsph.edu23082028http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007353http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007232http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007231http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007352http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007351http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007350http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007346http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007345http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007344http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007343http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007349http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007348http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007347http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007360http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007364http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007243http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007320http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007363http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007242http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007241http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007362http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007240http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007361http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007236http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007357http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007313http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007356http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007235http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007234http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007355http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007233http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007354http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007317http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007239http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007316http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007359http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007238http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007315http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007358http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007314http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007237http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007319http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007318http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007250http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007371http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007370http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007375http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007331http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007254http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007330http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007374http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007253http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007252http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007373http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007372http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007251http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007247http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007324http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007368http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007323http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007246http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007367http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007245http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007322http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007321http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007365http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007244http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007328http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007327http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007249http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007326http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007248http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007325http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007369http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007329http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007382http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007381http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007380http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007342http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007341http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007340http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007384http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007383http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007379http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007335http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007334http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007378http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007377http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007333http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007376http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007332http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007255http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007339http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007338http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007337http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007336Johns Hopkins University School of MedicineNot availableGPMDBMalaria morbidity and mortality caused by both Plasmodium falciparum and Plasmodium vivax extend well beyond the African continent, and although P. vivax causes between 80 and 300 million severe cases each year, vivax transmission remains poorly understood. Plasmodium parasites are transmitted by Anopheles mosquitoes, and the critical site of interaction between parasite and host is at the mosquito's luminal midgut brush border. Although the genome of the "model" African P. falciparum vector, Anopheles gambiae, has been sequenced, evolutionary divergence limits its utility as a reference across anophelines, especially non-sequenced P. vivax vectors such as Anopheles albimanus. Clearly, technologies and platforms that bridge this substantial scientific gap are required in order to provide public health scientists with key transcriptomic and proteomic information that could spur the development of novel interventions to combat this disease. To our knowledge, no approaches have been published that address this issue. To bolster our understanding of P. vivax-An. albimanus midgut interactions, we developed an integrated bioinformatic-hybrid RNA-Seq-LC-MS/MS approach involving An. albimanus transcriptome (15,764 contigs) and luminal midgut subproteome (9,445 proteins) assembly, which, when used with our custom Diptera protein database (685,078 sequences), facilitated a comparative proteomic analysis of the midgut brush borders of two important malaria vectors, An. gambiae and An. albimanus.A bioinformatics approach for integrated transcriptomic and proteomic comparative analyses of model and non-sequenced anopheline vectors of human malaria parasites.Ubaida Mohien Ceereena C,Colquhoun David R DR,Mathias Derrick K DK,Gibbons John G JG,Armistead Jennifer S JS,Rodriguez Maria C MC,Rodriguez Mario Henry MH,Edwards Nathan J NJ,Hartler Jürgen J,Thallinger Gerhard G GG,Graham David R DR,Martinez-Barnetche Jesus J,Rokas Antonis A,Dinglasan Rhoel R RR,Ubaida Mohien Ceereena C,Colquhoun David R DR,Mathias Derrick K DK,Gibbons John G JG,Armistead Jennifer S JS,Rodriguez Maria C MC,Rodriguez Mario Henry MH,Edwards Nathan J NJ,Hartler Jürgen J,Thallinger Gerhard G GG,Graham David R DR,Martinez-Barnetche Jesus J,Rokas Antonis A,Dinglasan Rhoel R RR,Dinglasan Rhoel R RR,Ubaida Mohien Ceereena C, Colquhoun David R DR, Mathias Derrick K DK, Gibbons John G JG, Armistead Jennifer S JS, Rodriguez Maria C MC, Rodriguez Mario Henry MH, Edwards Nathan J NJ, Hartler Jürgen J, Thallinger Gerhard G GG, Graham David R DR, Martinez-Barnetche Jesus J, Rokas Antonis A, Dinglasan Rhoel R RR, Dinglasan Rhoel R RRParasite., human being, Other pernicious complications of malaria, Plasmodium, Remittent, Malarial fever (finding), Homo sapiense, Plasmodium Infections, Homo spaiens, Malaria, unspecified, Homo sapien, Disease due to Plasmodiidae (disorder), Homo sapian, Induced malaria (disorder), Homo sapians, Other malaria, Infection, MALARIA NOS, NOS, Unspecified malaria (disorder), Homo sapeins, [X]Unspecified malaria (disorder), Other malaria (disorder), MALARIA COMPLICATED NEC, [X]Unspecified malaria, Homo sapients, Disease due to Plasmodiidae, Unspecified malaria, Plasmodium Infection, Infections, PLASMODIUM INFECT, Humo sapiens, man, Homo sapines, human, INFECT PLASMODIUM, Homo spiens, "human" EXACT genbank_common_name [], Plasmodiosis, Homo sapience, Homo sampiens, MALARIA NEC, Fever, induced malaria, Malaria (disorder), Remittent Fever, Marsh, Home sapiens, Malarial fever, Paludism, Marsh Feverliquid chromatography tandem mass spectroscopy, OCT, Phenylethylbarbiturate, mol, PLXN2, Transcriptome Profile, (H2, Gene Expression Profile, Remittent, CCC1(C(=O)NC(=O)NC1=O)c1ccccc1, Gene, Profiles, Malarial fever (finding), electronic data file, PHENYLETHYLMALONYLUREA, LC-MS-MS, 13, 14, Plasmodium Infections, 15, Readability, 16, unspecified, Phenylethylbarbituric Acid, Disease due to Plasmodiidae (disorder), Other malaria, LC-MSMS, Gene Products, 6-trione, 4, Phenylethylbarbitursaeure, [X]Unspecified malaria (disorder), Flies, LCMSMS, Disease due to Plasmodiidae, Infections, cell, PLASMODIUM INFECT, Profile, True Fly, Fly, 5-Phenyl-5-ethylbarbituric acid, Anopheles gambiae, 2810428A13Rik, proteins, 5-ethyl-5-phenyl-2, Signatures, Phenobarbitol, Plasmodiosis, Dipteras, Plasmodiums, 5H)-pyrimidinetrione, Expression Signature, Fever, 6(1H, Transcriptomes, 3H, mKIAA0463, Paludism, Marsh Fever, LC-MS2, flies, data, PlexA2, Transcriptome, InChI=1/C12H12N2O3/c1-2-12(8-6-4-3-5-7-8)9(15)13-11(17)14-10(12)16/h3-7H, True, Other pernicious complications of malaria, Marsh., Expression Profiles, Proteins, LC-MS/MS, Plasmodium, Mosquito, InChIKey=DDBREPKUVSBGFI-KGCNKATMCV, AA589422, 2H2, Cell, whole transcriptome, C12H12N2O3, Gene Expression, polypeptide, Malaria, LC/MS/MS, Experiment, AW457381, Phenobarbituric Acid, Induced malaria (disorder), Expression Signatures, Gene Expression Signatures, Protein, proteomic analysis, Infection, computer data file, Mosquitoes, MALARIA NOS, NOS, Unspecified malaria (disorder), Gene Expression Signature, Expression Profile, Other malaria (disorder), Data Base, Mosquitos, Transcriptome Profiles, Phenylaethylbarbitursaeure, MALARIA COMPLICATED NEC, [X]Unspecified malaria, Luminal, True Flies, Unspecified malaria, Plasmodium Infection, 17)/f/h13-14H, 5-Ethyl-5-phenylbarbituric acid, 5-Ethyl-5-phenyl-pyrimidine-2, 5H)-trione, Entire primitive midgut, Understanding, 1H3, INFECT PLASMODIUM, Protein Gene Products, Gene Proteins, 5-ethyl-5-phenylpyrimidine-2, Parasite, liquid chromatography-tandem mass spectroscopy, Gene Expression Profiles, MALARIA NEC, induced malaria, Malaria (disorder), Remittent Fever, liquid chromatography tandem mass spectrometry, mesenteron, Signature, Plxn2, Malarial fever, PhenobarbitoneParasite., human being, Other pernicious complications of malaria, Plasmodium, Remittent, Malarial fever (finding), Homo sapiense, Plasmodium Infections, Homo spaiens, Malaria, unspecified, Homo sapien, Disease due to Plasmodiidae (disorder), Homo sapian, Induced malaria (disorder), Homo sapians, Other malaria, Infection, MALARIA NOS, NOS, Unspecified malaria (disorder), Homo sapeins, [X]Unspecified malaria (disorder), Other malaria (disorder), MALARIA COMPLICATED NEC, [X]Unspecified malaria, Homo sapients, Disease due to Plasmodiidae, Unspecified malaria, Plasmodium Infection, Infections, PLASMODIUM INFECT, Humo sapiens, man, Homo sapines, human, INFECT PLASMODIUM, Homo spiens, "human" EXACT genbank_common_name [], Plasmodiosis, Homo sapience, Homo sampiens, MALARIA NEC, Fever, induced malaria, Malaria (disorder), Remittent Fever, Marsh, Home sapiens, Malarial fever, Paludism, Marsh Feverliquid chromatography tandem mass spectroscopy, Phenylethylbarbiturate, host organism, single-organism developmental process, Gene Expression Profile, CCC1(C(=O)NC(=O)NC1=O)c1ccccc1, Profiles, ras p21 protein activator, Case Fatality Rates, 13, Death Rates, 14, 15, Readability, unspecified, 16, Phenylethylbarbituric Acid, Disease due to Plasmodiidae (disorder), Other malaria, PKWS, 6-trione, 4, Excess Mortalities, Flies, 1902, human disease, LCMSMS, Disease due to Plasmodiidae, Genomes, PLASMODIUM INFECT, Fly, proteins, 5-ethyl-5-phenyl-2, Community Health, Signatures, Plasmodiosis, Expression Signature, Fever, falciparums, 6(1H, Transcriptomes, CM-AVM, Homo sapiens disease, 3H, Age-Specific Death Rate, Marsh, Bridge-1, LC-MS2, close to, Marsh., Expression Profiles, Plasmodium, LC-MS/MS, RNA-seq, Mosquito, RASA, 2H2, Gene Expression, Malaria, Mortality, Induced malaria (disorder), Expression Signatures, Diseases, NOS, Unspecified malaria (disorder), GAP, p120RASGAP, Other malaria (disorder), Expression Profile, Plasmodium vivaxs, Mosquitos, Bridge, Transcriptome Profiles, MALARIA COMPLICATED NEC, Epistemology, True Flies, Anopheles gambiae S, Unspecified malaria, death rate, Plasmodium Infection, Plasmodium (Laverania) falciparum, 5-Ethyl-5-phenyl-pyrimidine-2, Mortalities, 5H)-trione, Entire primitive midgut, whole genome, 1H3, INFECT PLASMODIUM, 5-ethyl-5-phenylpyrimidine-2, Health, Mortality Rate, Parasite, liquid chromatography-tandem mass spectroscopy, MALARIA NEC, Ras p21 protein activator, Malaria (disorder), Preventive Medicine & Public Health, Preventive Medicine and Public Health, Mortality Determinant, Community, Transcriptome Profile, Mortality Declines, Excess, (H2, Remittent, GTPase-activating protein, Gene, Malarial fever (finding), PHENYLETHYLMALONYLUREA, LC-MS-MS, Age-Specific Death, Mortality Determinants, Plasmodium Infections, Rate, Determinant, vivax, LC-MSMS, Anopheles gambiae sensu stricto, Gene Products, Case Fatality, Phenylethylbarbitursaeure, [X]Unspecified malaria (disorder), Differential Mortality, Anopheles gambiae Giles, Excess Mortality, Infections, RasGAP, Profile, True Fly, 5-Phenyl-5-ethylbarbituric acid, Anopheles gambiae, African malaria mosquito, Age-Specific, Phenobarbitol, Dipteras, Plasmodiums, 5H)-pyrimidinetrione, Mortality Rates, mortality rate, African unspecified, Plasmodium falciparums, Age Specific Death Rate, Paludism, Marsh Fever, Morbidities, flies, Transcriptome, Other pernicious complications of malaria, Mortality Decline, InChI=1/C12H12N2O3/c1-2-12(8-6-4-3-5-7-8)9(15)13-11(17)14-10(12)16/h3-7H, True, Proteins, InChIKey=DDBREPKUVSBGFI-KGCNKATMCV, RASGAP, near to, whole transcriptome, C12H12N2O3, development, polypeptide, Case Fatality Rate, LC/MS/MS, survival, Phenobarbituric Acid, Public, Gene Expression Signatures, Protein, proteomic analysis, Infection, MALARIA NOS, Mosquitoes, Gene Expression Signature, RASA1, Determinants, Death, p120GAP, Environment, Data Base, Phenylaethylbarbitursaeure, [X]Unspecified malaria, Rates, Death Rate, Luminal, 17)/f/h13-14H, 5-Ethyl-5-phenylbarbituric acid, Anopheles gambia, Understanding, Age-Specific Death Rates, CMAVM, Protein Gene Products, Gene Proteins, Differential, Declines, approaches, Gene Expression Profiles, induced malaria, vicinity of, Remittent Fever, Decline, liquid chromatography tandem mass spectrometry, mesenteron, Signature, malaria parasite P. falciparum, Malarial fever, malaria parasite P. vivax, Differential Mortalities, Phenobarbitone2405http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007339dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0074028377544725480.01.0falseA bioinformatics approach for integrated transcriptomic and proteomic comparative analyses of model and non-sequenced anopheline vectors of human malaria parasitesPXD ID: PXD000062, experiment: GambiaeOG3, data file: gambiaef11.mzxml. Data published as part of Mol Cell Proteomics. 2012 Oct 17 .From the Abstract: {{i}}... Plasmodium parasites are transmitted by Anopheles mosquitoes and the critical site of interaction is at the mosquito"s luminal midgut brush border. ... To bolster our understanding of P. vivax-An. albimanus midgut interactions, we developed an integrated bioinformatic-hybrid RNASeq-LC-MS/MS approach involving An. albimanus transcriptome (15,764 contigs) and luminal midgut subproteome (9,445 proteins) assembly, which when combined with our custom Diptera protein database (685,078 sequences), facilitated a comparative proteomic analysis of the midgut brush borders of two important malaria vectors, A. gambiae and A. albimanus.{{/i}}2012-11-02GPM1121000733923082028PXD000062PXD000062