GPMDB00178OtherEne IV, et al.InstrumentNot AvailableNot availableCandida_albicansAl.Brown@abdn.ac.uk22997008Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeenhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010998http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010999http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011009http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011008http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010996http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011007http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007420http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010997http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011006http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011005http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010995http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011004http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010992http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010993http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011003http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011002http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010990http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010991http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011001http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210011000http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010969http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010967http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010968http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010966http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010978http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010979http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010976http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010977http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010974http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010975http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010972http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010973http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010970http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010971http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010989http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010987http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010988http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010985http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010986http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007416http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010983http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010984http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007415http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010981http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010982http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007419http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010980http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007418http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210007417Not availableGPMDBThe major fungal pathogen Candida albicans can occupy diverse microenvironments in its human host. During colonization of the gastrointestinal or urogenital tracts, mucosal surfaces, bloodstream, and internal organs, C. albicans thrives in niches that differ with respect to available nutrients and local environmental stresses. Although most studies are performed on glucose-grown cells, changes in carbon source dramatically affect cell wall architecture, stress responses, and drug resistance. We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome. The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells. Moreover, changes in other proteins (e.g. Als2, Gca1, Phr1, Sap9) correlated with the increased adherence and biofilm formation of lactate-grown cells. We identified mating and pheromone-regulated proteins that were exclusive to lactate-grown cells (e.g. Op4, Pga31, Pry1, Scw4, Yps7) as well as mucosa-specific and other niche-specific factors such as Lip4, Pga4, Plb5, and Sap7. The analysis of the corresponding null mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance. Therefore, the cell wall proteome and secretome display considerable plasticity in response to carbon source. This plasticity influences important fitness and virulence attributes known to modulate the behavior of C. albicans in different host microenvironments during infection.Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.Ene Iuliana V IV,Heilmann Clemens J CJ,Sorgo Alice G AG,Walker Louise A LA,de Koster Chris G CG,Munro Carol A CA,Klis Frans M FM,Brown Alistair J P AJ,Ene Iuliana V IV, Heilmann Clemens J CJ, Sorgo Alice G AG, Walker Louise A LA, de Koster Chris G CG, Munro Carol A CA, Klis Frans M FM, Brown Alistair J P AJCell Walls, C, Wall, 6C, carbon atom, drug susceptibility/resistance, Candida stellatoidea type I., InChI=1/C, carbone, Cell, Carbon 12, Drug, carbono, Vitreous, InChIKey=OKTJSMMVPCPJKN-UHFFFAOYAS, Kohlenstoff, Vitreous Carbon, Candida albican, Carbon-12, Carbon, carbonium, Resistance, albicans, Candida stellatoidea, Candida, Proteomes, drug resistance, WallsIGF-I, Reproductive, data, steel factor, Rab interactor activity, Infertile, Slf, hematopoietic growth factor KL, Somatomedin-C, somatomedin, AI836084, FPH2, wall of organ, electronic data file, Cell, sKITLG, Pancreatic insufficiency and bone marrow dysfunction., Fe-containing alcohol dehydrogenase, SDS, Rab escort protein activity, HOT, mechano growth factor, Infertile (finding), organ wall, computer data file, sterile, Igf-1, Walls, Cell Walls, Wall, SHEP7, ADHFe1, mast cell growth factor, 1.1.99.24, hot, ADH8, Stem cell factor, somatomedin-C, SF, 4733401P19Rik, SWDS, Kitl, Shwachman-Bodian-Diamond syndrome, Shwachman syndrome, STAT5, CGI-97, Mast cell growth factor, Alcohol dehydrogenase iron-containing protein 1, stem cell factor, KL-1, IGF1, MGF, Sterility, Reproductive Sterility, c-Kit ligand, high temperature, Mechano growth factor, KITLG, Soluble KIT ligand, SCF, Sl, Proteomes, REP, wallCell Walls, C, Wall, 6C, carbon atom, drug susceptibility/resistance, Candida stellatoidea type I., InChI=1/C, carbone, Cell, Carbon 12, Drug, carbono, Vitreous, InChIKey=OKTJSMMVPCPJKN-UHFFFAOYAS, Kohlenstoff, Vitreous Carbon, Candida albican, Carbon-12, Carbon, carbonium, Resistance, albicans, Candida stellatoidea, Candida, Proteomes, drug resistance, Wallsgluco-hexose, evt-1, LIP4, host organism, human being, C6H12O6, determination, Glucose, increased resistance, MCG10, Processes, Glukose, mating, drug susceptibility/resistance, Monohydrate, Gene, (DL)-Isomer, InChI=1/C, Homo sapiense, supernumerary, InChIKey=OKTJSMMVPCPJKN-UHFFFAOYAS, Homo spaiens, Homo sapien, Carbon-12, Homo sapians, responsivity, Dextrose, 2-hydroxypropanoate, Gene Products, PHR1, Phr1, Mood, antifungal agent, drug resistance, antifungal drugs, Walls, Cell Walls, reactivity, C, increased, viral infection, Homo sapients, cell fusion, C130061D10Rik, Candida stellatoidea type I, Moods, nutrients, carbon atom, proteins, virus process, behavioral response to stimulus, man, region of mucosa, mucous membrane, mucosa of organ, Kohlenstoff, PHRET1, Homo sapience, Homo sampiens, Carbon, mucosa of organ part, ALS2CR6, Candida, PLSJ, Behaviors, Home sapiens, behaviour, Anhydrous, lactate, Pam, single-organism behavior, AI315628, ALS, (beta-D)-Isomer, Process, R75243, Affects, Proteins, AW107953, carbone, Cell, CHT1, Acceptance Processes, Juvenile, carbono, Vitreous, polypeptide, AU023734, Acceptance Process, KPL1, Homo sapian, mucosal region, Protein, IAHSP, chemical analysis, Resistance, Pathogenicity, ALS2, CHOT1, Homo sapeins, (alpha-D)-Isomer, Phret1, L Glucose, D-Glucose, Acceptance, Wall, CRT, AW546647, growth pattern, tunica mucosa, 6C, non-developmental growth, increased number, organ mucosa, CBP, Humo sapiens, Infections., Homo sapines, ALSJ, human, Anhydrous Dextrose, Carbon 12, Drug, Protein Gene Products, CT1, Fitness and Exercise, Gene Proteins, present in greater numbers in organism, Vitreous Carbon, high resistance to, Homo spiens, Candida albican, "human" EXACT genbank_common_name [], L-Glucose, behavioural response to stimulus, D Glucose, carbonium, (L)-Isomer, albicans, Candida stellatoidea, regulation, virulence, assay, response, Glucose Monohydrate, E430016J11Rik, Proteomes, RWDD5, PIR1, accessory, Glc1708http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210010990dbgap_ncbi~0patentfamilies~0rfam~0uniprot~3merops~0complex-portal~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0052436767427513881.00.0falseCarbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicansPXD ID: PXD000008. Original data file: CW1113_q1 GluLac walls rep 2.mgf. Data published as part of Proteomics 2012 12:3164-79 . From Proteomexchange: {{i}}Cell wall and secretome preparation. C. albicans RM1000 cells grown to mid-exponential phase were harvested by centrifugation. The resulting supernatant was sterile-filtered and then concentrated on 10-kDcut-off filters (Amicon Ultra-15 Centrifugal filter units, Millipore) as described previously. To analyse the wall proteome, cell walls were isolated by hot SDS-extraction. ...{{/i}}2013-02-20GPM1121001099022997008PXD000008PXD000008