GPMDB00155OtherDohyun Han, et al.InstrumentNot AvailableNot availableMouse, Abelson_murine_leukemia_virus, Ectromelia_virus, Mouse_mammary_tumor_virus, Mouse_parvovirus_1, Mouse_parvovirus_2, Mouse_parvovirus_3, Mouse_parvovirus_4a, Mouse_parvovirus_5a, Murid_herpesvirus_1, Murid_herpesvirus_2, Murid_herpesvirus_4, Murine_adenovirus_3, Murine_adenovirus_a, Murine_hepatitis_virus_a59, Murine_hepatitis_virus_jhm, Murine_norovirus_1, Murine_osteosarcoma_virus, Murine_pneumotropic_virus, Murine_polyomavirus, Murine_type_c_retrovirus23943505hdh03@snu.ac.krhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021377http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021376http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021379http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021378http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021395http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021373http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021372http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021394http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021375http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021396http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021374http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021391http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021390http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021371http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021393http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021392http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021369http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021366http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021388http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021387http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021365http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021368http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021367http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021389http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021362http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021384http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021383http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021361http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021386http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021364http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021385http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021363http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021380http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021382http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021381Seoul National University College of MedicineNot availableGPMDBMicroglia are major immune cells in the central nervous system. A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution MS quickly and cost efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis. All MS data have been deposited in the ProteomeXchange with identifier PXD000168.In-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.Han Dohyun D,Moon Sungyoon S,Kim Yikwon Y,Kim Jihye J,Jin Jonghwa J,Kim Youngsoo Y,Han Dohyun D, Moon Sungyoon S, Kim Yikwon Y, Kim Jihye J, Jin Jonghwa J, Kim Youngsoo YMus musculus, 3.4.22.-, MF.microglia.CNS, mixed glial cells (predominantly microglia), microglial cell, Caspase-14 subunit p10, Hortega cell, Laboratory, Swiss, mice, Mus domesticus, brain-resident macrophage, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, backward, "mouse" EXACT common_name [], MICE, House Mouse, microgliocyte, microglia, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], reversed., House, Mus, Mini-ICE, proteomic analysis, Mus musculus domesticus, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, brain macrophage, hortega cells, house mouse, Mice, Laboratory Mousemixed glial cells (predominantly microglia), fees, data, Neurologic Disorder, determination, brain-resident macrophage, Neurologic Diseases, Degenerative Neurologic Disorders, Neurologic Degenerative Condition, function, Spectrum Analyses, Spectrum Analysis, central nervous system degenerative disorder, Neurodegenerative Diseases, Degenerative Neurologic Disorder, Spectroscopy, polypeptide, Experiment, Neurologic Disorders, Associations, chemical analysis, Mass, Analysis, Mass Spectrum Analyses, finances, Mass Spectroscopy, Nervous System Degenerative Diseases, Mass Spectrum Analysis, MF.microglia.CNS, study, Degenerative Conditions, Mass Spectrum, microglial cell, Nervous System, Degenerative Condition, inflammatory response, Hortega cell, Analyses, Degenerative Diseases, Spectrometry, salaries, inflammation, Neurologic Degenerative Conditions, proteins, Neurologic Disease, financing, Neurodegenerative Disorder, microgliocyte, Neurodegenerative Disorders, microglia, funding, Neurologic, cost, Neurologic Degenerative Diseases, Phagocytoses., Degenerative Neurologic Disease, Degenerative, Inflammation, Degenerative Neurologic Diseases, Neurologic Degenerative Disease, Neurodegenerative Disease, Central Nervous System, brain macrophage, hortega cells, Spinal Cord, assay, financial management, ProteomesMus musculus, 3.4.22.-, MF.microglia.CNS, mixed glial cells (predominantly microglia), microglial cell, Caspase-14 subunit p10, Hortega cell, Laboratory, Swiss, mice, Mus domesticus, brain-resident macrophage, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, backward, "mouse" EXACT common_name [], MICE, House Mouse, microgliocyte, microglia, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], reversed., House, Mus, Mini-ICE, proteomic analysis, Mus musculus domesticus, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, brain macrophage, hortega cells, house mouse, Mice, Laboratory MouseBrain, mixed glial cells (predominantly microglia), fees, Neurologic Disorder, neuraxis, Central, determination, brain-resident macrophage, Cerebrospinal Axis, Neurologic Diseases, Degenerative Neurologic Disorders, Neurologic Degenerative Condition, function, Cell, Central Nervous Systems, brain structure, central nervous system degenerative disorder, Cerebrospinal, Central Nervous, Neurodegenerative Diseases, Degenerative Neurologic Disorder, polypeptide, Nervous Systems, Neurologic Disorders, Associations, cerebrospinal axis, Systems, chemical analysis, central nervous system, synganglion, Phagocytoses, finances, Nervous System Degenerative Diseases, MF.microglia.CNS, Cerebrospinal axis, study, Degenerative Conditions, microglial cell, Nervous System, Degenerative Condition, inflammatory response, CNS, systema nervosum centrale, Hortega cell, Degenerative Diseases, System, salaries, inflammation, Neurologic Degenerative Conditions, proteins, Neurologic Disease, financing, Neuraxis, Neurodegenerative Disorder, microgliocyte, Neurodegenerative Disorders, microglia, Axis, funding, Neurologic, cost, Entire central nervous system, Neurologic Degenerative Diseases, Degenerative Neurologic Disease, Cerebrospinal Axi, Degenerative, Inflammation, data., Degenerative Neurologic Diseases, Neurologic Degenerative Disease, Neurodegenerative Disease, Axi, Central Nervous System, brain macrophage, hortega cells, Spinal Cord, assay, financial management, Proteomes1505http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210021378dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.00462677359654534250.01.0falseIn-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.Data from ProteomeXchange, PXD ID: PXD000168. Experiment: BV_2_High_pH_biological_1, file: 20121208_L_4.mzML. Published as part of Proteomics. 2013 Aug 14 . From the Abstract: {{i}} ... A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution mass spectrometry quickly and cost-efficiently. By bioinformatics analysis, the BV-2 proteome is a valuable resource for studies of microglial function, such as in the immune response, inflammatory response, and phagocytosis ... {{/i}}2013-09-11GPM1121002137823943505PXD000168PXD000168