GPMDB00233OtherKukuczka B, et al.InstrumentNot AvailableNot availablePhyscomitrella_patens24948831mhippler@uni-muenster.dehttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028490http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028491http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028492http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028493http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028494http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028495http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028485http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028486http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028520http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028487http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028521http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028488http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028522http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028489http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028523http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028524http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028525http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028526http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028527http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028528http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028529http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028480http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028481http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028482http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028484http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028474http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028475http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028476http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028477http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028510http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028478http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028511http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028512http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028479http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028513http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028514http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028515http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028516http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028517http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028518http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028519http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028470http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028471http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028472http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028473http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028540http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028541http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028542http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028543http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028466http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028500http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028467http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028544http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028468http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028501http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028502http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028469http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028503http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028504http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028505http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028506http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028507http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028508http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028509http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028496http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028497http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028530http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028531http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028498http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028532http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028499http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028533http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028534http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028535http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028536http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028537http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028538http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028539Institute of Plant Biology and Biotechnology, University of Munster, et al.Not availableGPMDBTo investigate the functional importance of Proton Gradient Regulation5-Like1 (PGRL1) for photosynthetic performances in the moss Physcomitrella patens, we generated a pgrl1 knockout mutant. Functional analysis revealed diminished nonphotochemical quenching (NPQ) as well as decreased capacity for cyclic electron flow (CEF) in pgrl1. Under anoxia, where CEF is induced, quantitative proteomics evidenced severe down-regulation of photosystems but up-regulation of the chloroplast NADH dehydrogenase complex, plastocyanin, and Ca(2+) sensors in the mutant, indicating that the absence of PGRL1 triggered a mechanism compensatory for diminished CEF. On the other hand, proteins required for NPQ, such as light-harvesting complex stress-related protein1 (LHCSR1), violaxanthin de-epoxidase, and PSII subunit S, remained stable. To further investigate the interrelation between CEF and NPQ, we generated a pgrl1 npq4 double mutant in the green alga Chlamydomonas reinhardtii lacking both PGRL1 and LHCSR3 expression. Phenotypic comparative analyses of this double mutant, together with the single knockout strains and with the P. patens pgrl1, demonstrated that PGRL1 is crucial for acclimation to high light and anoxia in both organisms. Moreover, the data generated for the C. reinhardtii double mutant clearly showed a complementary role of PGRL1 and LHCSR3 in managing high light stress response. We conclude that both proteins are needed for photoprotection and for survival under low oxygen, underpinning a tight link between CEF and NPQ in oxygenic photosynthesis. Given the complementarity of the energy-dependent component of NPQ (qE) and PGRL1-mediated CEF, we suggest that PGRL1 is a capacitor linked to the evolution of the PSII subunit S-dependent qE in terrestrial plants.To investigate the functional importance of Proton Gradient Regulation5-Like1 (PGRL1) for photosynthetic performances in the moss Physcomitrella patens, we generated a pgrl1 knockout mutant. Functional analysis revealed diminished nonphotochemical quenching (NPQ) as well as decreased capacity for cyclic electron flow (CEF) in pgrl1. Under anoxia, where CEF is induced, quantitative proteomics evidenced severe down-regulation of photosystems but up-regulation of the chloroplast NADH dehydrogenase complex, plastocyanin, and Ca²⁺ sensors in the mutant, indicating that the absence of PGRL1 triggered a mechanism compensatory for diminished CEF. On the other hand, proteins required for NPQ, such as light-harvesting complex stress-related protein1 (LHCSR1), violaxanthin de-epoxidase, and PSII subunit S, remained stable. To further investigate the interrelation between CEF and NPQ, we generated a pgrl1 npq4 double mutant in the green alga Chlamydomonas reinhardtii lacking both PGRL1 and LHCSR3 expression. Phenotypic comparative analyses of this double mutant, together with the single knockout strains and with the P. patens pgrl1, demonstrated that PGRL1 is crucial for acclimation to high light and anoxia in both organisms. Moreover, the data generated for the C. reinhardtii double mutant clearly showed a complementary role of PGRL1 and LHCSR3 in managing high light stress response. We conclude that both proteins are needed for photoprotection and for survival under low oxygen, underpinning a tight link between CEF and NPQ in oxygenic photosynthesis. Given the complementarity of the energy-dependent component of NPQ (qE) and PGRL1-mediated CEF, we suggest that PGRL1 is a capacitor linked to the evolution of the PSII subunit S-dependent qE in terrestrial plants.Proton Gradient Regulation5-Like1-Mediated Cyclic Electron Flow Is Crucial for Acclimation to Anoxia and Complementary to Nonphotochemical Quenching in Stress Adaptation.Kukuczka Bernadeta B,Magneschi Leonardo L,Petroutsos Dimitris D,Steinbeck Janina J,Bald Till T,Powikrowska Marta M,Fufezan Christian C,Finazzi Giovanni G,Hippler Michael M,Kukuczka Bernadeta B, Magneschi Leonardo L, Petroutsos Dimitris D, Steinbeck Janina J, Bald Till T, Powikrowska Marta M, Fufezan Christian C, Finazzi Giovanni G, Hippler Michael MNegatrons, Acclimation., Electron, Positrons, Fast Electrons, Fast, Electrons, Negatron, Fast Electron, PositronCAS1, Spexin hormone, Liver regeneration-related protein LRRGT00060, Set1, determination, AI461847, Physcomitrium patens, Crkas, Speech-language disorder type 1, AP-1, Pflanze, number, P120CAS, presence, Downregulation, viridiplantae, Plastocyanin, 2610100P18Rik, CEF, reduced, subnumerary, Pgi, Set1A, Gpi-1, CRKAS, p120(ctn), present in fewer numbers in organism, plantae, AA407533, KMT2F, Junc, Gpi-1r, Nlk, Receptor Up-Regulation, Gpi-1s, Up Regulation, Plastocyanine, absent from organism, Phi, Gpi-1t, NDH, decreased number, Fast, Chloroplast, Etioplast, decreased, Capts, AI385681, c-Jun, NPQ 53-70, CASS1, Electrons, Positron, Gpi, P130Cas, P130CAS, data, Physcomitrella patens subsp. patens, p120, Negatron, c-jun, Up-Regulation (Physiology), XPO2, Xpo2, Negatrons, CEF., Positrons, Spexin-1, MF, Etioplasts, plastocyanin, Spx, Amf, Spexin-2, Down Regulation, count in organism, CTNND, Experiment, Down-Regulation, mOC-X, count, p120(CTN), Receptor Down-Regulation, Down-Regulation (Physiology), chemical analysis, Fast Electron, Silver, Cas, CAS, NK|GPI, Fast Electrons, Plastocyanins, NK, Speech and language disorder with orofacial dyspraxia, Org, ORG, Silver Plastocyanin, absence, Upregulation, NPQ, Npq, Plant, Gpi1-r, Gpi1-s, Electron, P120CTN, Gpi1-t, p120(CAS), AP1, CSE1, Neuropeptide Q, p120(cas), p120 catenin, assay, Receptor, quantitative, Developmental verbal dyspraxia, Bglap-rs1, Cadherin-associated Src substrate, Gpi1s, presence or absence in organismNegatrons, Acclimation., Electron, Positrons, Fast Electrons, Fast, Electrons, Negatron, Fast Electron, Positronforelimb autopodium, Spexin hormone, Liver regeneration-related protein LRRGT00060, land plants., determination, Physcomitrium patens, Chlamydomonas, InChI=1/Ca/q+2, number, Gene, reinhardtii, Visible Light, Reductive Pentose Phosphate Cycle, presence, Downregulation, Photosynthetic Carbon Fixation, Plastocyanin, CEF, Ca2+, reduced, forelimb autopod, dioxygene, Roles, subnumerary, calcium(2+) ion, responsivity, violaxanthin deepoxidase, Photosynthesis Dark Reactions, Cycle, Acclimation, Gene Products, Concepts, light, Low, present in fewer numbers in organism, Sprains, Carbon Fixation, O=O, reactivity, reinhardius, me75, CALCIUM ION, Receptor Up-Regulation, NADH:plastoquinone reductase complex, HAND, Up Regulation, Plastocyanine, absent from organism, Oxygen-16, proteins, plants, Visible, decreased number, Calvin-Benson-Bassham, D17Mit170, Fast, T1, Chloroplast, Etioplast, foot, hand, antenna complex, InChIKey=BHPQYMZQTOCNFJ-UHFFFAOYAP, Chlamydomonas reihhardtii, decreased, NADH dehydrogenase complex (quinone), Complex I, time of survival, Chlamydomonas reinhardii, Role Concepts, Calvin-Benson-Bassham Cycle, Calvin, Strains, TR2, NPQ 53-70, Dark Reactions of Photosynthesis, Chlamydomonas smithii, Ca, molecular oxygen, InChI=1/O2/c1-2, Electrons, Positron, forefoot of quadruped, data, Radiation, Oxygen 16, cou, Physcomitrella patens subsp. patens, Photosynthetic, Negatron, Proteins, Sprain, Photosynthesis Dark Reaction, Up-Regulation (Physiology), O(2), Negatrons, Light, Positrons, terminal segment of free upper limb, Calvin Benson Cycle, Spexin-1, Etioplasts, plastocyanin, Spx, Chlamydomonas reinhardtius, Tl3, Spexin-2, CD258, Tl2, Down Regulation, doubly charged positive ion, Concept, polypeptide, count in organism, Down-Regulation, Lr, paw, LIGHT, Role Concept, survival, count, Receptor Down-Regulation, NADH dehydrogenase complex (plastoquinone), Down-Regulation (Physiology), fore-paw, chemical analysis, Protein, InChIKey=MYMOFIZGZYHOMD-UHFFFAOYAM, Fast Electron, Strain, Role, Silver, Calvin-Benson Cycle, Fast Electrons, Plastocyanins, forefeet, plastid NADH dehydrogenase complex (plastoquinone), OXYGEN MOLECULE, Visible Radiations, Radiations, O2, Disauerstoff, dioxygen, Visible Radiation, Silver Plastocyanin, absence, HVEML, death rate, Upregulation, NPQ, Npq, Photoradiation, Plant, Calvin Benson Bassham Cycle, Electron, Ca(2+), Chlamydomonas reinhardius, LTg, Hands, Protein Gene Products, Gene Proteins, calcium, higher plants, Photoradiations, Neuropeptide Q, calcium(II) cation, Calvin Cycle, fore foot, hand region, Bra, fore paw, forepaw, assay, Receptor, quantitative, response, NADH dehydrogenase complex (ubiquinone), forefoot, Calvin-Benson, Chlamydomonas smithii R.W.Howshaw & H.Ettl, Strains and Sprains, Chlamydomonas reinhardtii P.A.Dangeard, Dioxygen, presence or absence in organism2303http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028492dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0varsite~00.00.00.0070943861813695250.001.0falsePGRL1-mediated cyclic electron flow is crucial for acclimation to anoxia and complementary to non-photochemical quenching in stress adaptation.Data from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00384 PASS00384]]. Experiment: set1, file: BK_physco_16022013_1_27.mzML. Published as part of Plant Physiol. 2014 Jun 19 . From the Abstract: {{i}} To investigate the functional importance of PGRL1 for photosynthetic performances in the moss Physcomitrella patens, we generated a pgrl1 knockout mutant. Functional analysis revealed diminished non-photochemical quenching (NPQ) as well as decreased capacity for cyclic electron flow (CEF) in pgrl1. Under anoxia, where CEF is induced, quantitative proteomics evidenced severe down regulation of photosystems but up regulation of the chloroplast NDH complex, plastocyanin and CAS in the mutant, indicating that the absence of PGRL1 triggered mechanism compensatory for diminished CEF. ... {{/i}}2014-07-11GPM1121002849224948831