GPMDB00596OtherAn E, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mouse25044017nitalazarau@niaid.nih.govNIH, United Stateshttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028881http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028882http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028840http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028841http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028842http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028843http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028844http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028845http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028846http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028847http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028848http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028849http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028880http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028870http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028871http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028872http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028873http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028874http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028830http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028875http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028831http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028876http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028832http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028877http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028833http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028878http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028834http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028879http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028835http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028836http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028837http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028838http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028839http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028860http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028861http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028862http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028863http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028820http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028864http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028865http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028821http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028822http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028866http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028823http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028867http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028824http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028868http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028825http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028869http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028826http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028827http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028828http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028829http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028850http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028851http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028852http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028853http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028810http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028854http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028811http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028855http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028812http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028856http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028857http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028813http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028858http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028814http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028815http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028859http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028816http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028817http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028818Not availableGPMDBIn addition to forming macrophages and dendritic cells, monocytes in adult peripheral blood retain the ability to develop into osteoclasts, mature bone-resorbing cells. The extensive morphological and functional transformations that occur during osteoclast differentiation require substantial reprogramming of gene and protein expression. Here we employ -omic-scale technologies to examine in detail the molecular changes at discrete developmental stages in this process (precursor cells, intermediate osteoclasts, and multinuclear osteoclasts), quantitatively comparing their transcriptomes and proteomes. The data have been deposited to the ProteomeXchange with identifier PXD000471. Our analysis identified mitochondrial changes, along with several alterations in signaling pathways, as central to the development of mature osteoclasts, while also confirming changes in pathways previously implicated in osteoclast biology. In particular, changes in the expression of proteins involved in metabolism and redirection of energy flow from basic cellular function toward bone resorption appeared to play a key role in the switch from monocytic immune system function to specialized bone-turnover function. These findings provide new insight into the differentiation program involved in the generation of functional osteoclasts.Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling.An Eunkyung E,Narayanan Manikandan M,Manes Nathan P NP,Nita-Lazar Aleksandra A,An Eunkyung E, Narayanan Manikandan M, Manes Nathan P NP, Nita-Lazar Aleksandra AmRNA., number, INSDC_feature:mRNA, count in organism, messenger RNA, template RNA, quantitative, osteoclast cell development, count, protein_coding_transcript, presence, presence or absence in organismprojections, Interstitial Dendritic Cells, Dendritic Cells, data, Veiled Cells, scale tissue, mononuclear leukocyte, anatomical protrusion, whole blood, Monocyte Derived Macrophages, lamellae, Interdigitating, adult stage, Blood, anatomical process, peltate hair, osteoclast cell differentiation, lamina, flanges, Monocyte Derived, Interstitial, process of organ, Cell, osteogenic tissue, protrusion, monocytes, polypeptide, lamella, Plasmacytoid Dendritic Cells, Monocyte-Derived, Experiment, blood cells, Proteomes., Monocyte-Derived Macrophage, shelf, Interdigitating Dendritic Cell, bones, Dendritic Cell, scales, Interstitial Dendritic Cell, Adults, Odontoclasts, adult, flange, organ process, Monocyte-Derived Macrophages, Macrophage, osseous tissue, Plasmacytoid Dendritic Cell, adults, Peripheral Blood, scale, Plasmacytoid Dendritic, Veiled Cell, Osteoclast, Macrophages, Interdigitating Dendritic, Dendritic, portion of bone tissue, plant peltate hair, shelves, Veiled, Interdigitating Cells, Plasmacytoid, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, proteins, circulating cell, ridges, Odontoclast, Bones, projection, ridge, Reticuloendothelial System, Bone Marrow-Derived, process, processes, Monocyte, Cementoclasts, Interstitial Dendritic, circulating cells, mineralized bone tissue, bone, spine, Cementoclast, papilla, Cells, Bone Marrow-Derived Macrophages, Interdigitating Dendritic Cells, processus, Interdigitating Cell, calcium tissue, bone organ, laminae, OsseousmRNA., number, INSDC_feature:mRNA, count in organism, messenger RNA, template RNA, quantitative, osteoclast cell development, count, protein_coding_transcript, presence, presence or absence in organismprojections, biochemical pathways, Osteoclastic Bone Loss, Dendritic Cells, scale tissue, biological signaling, Metabolic Process, Plays, mononuclear leukocyte, Immune Systems, single-organism developmental process, determination, Monocyte Derived Macrophages, lamellae, Processes, Interdigitating, adult stage, Blood, peltate hair, Metabolic Concepts, Gene, Metabolic Processes, chondroclast, process of organ, osteogenic tissue, protrusion, Osteoclastic, monocytes, lamella, conclusions, Cementoclast., Roles, Metabolism, Monocyte-Derived Macrophage, Gene Products, symptoms, Concepts, Interdigitating Dendritic Cell, Losses, Dendritic Cell, Metabolism Concept, Phenomenon, Interstitial Dendritic Cell, Metabolism Phenomena, Bone Losses, adult, Toy, Resorptions, Plasmacytoid Dendritic, Osteoclast, Playthings, catabolism, Interdigitating Dendritic, Dendritic, plant peltate hair, Plasmacytoid, Metabolic Concept, Bone Marrow-Derived Macrophage, proteins, metabolic process resulting in cell growth, ridges, Bone Marrow-Derived, Monocyte, Cementoclasts, Interstitial Dendritic, circulating cells, Immune, Loss, Puppets, Resorption, Cementoclast, papilla, signaling process, Role Concepts, Play, biotransformation, laminae, Catabolism, Puppet, single organism signaling, Interstitial Dendritic Cells, incorporation, screening, Veiled Cells, data, anatomical protrusion, findings, whole blood, degradation, Process, Bone Loss, Osteoclastic Bone Losses, anatomical process, metabolism resulting in cell growth, Proteins, osteoclast cell differentiation, lamina, flanges, Bone Resorptions, Monocyte Derived, function, Interstitial, Osteoclastic Bone, Cell, Concept, Metabolic Phenomena, polypeptide, development, Plasmacytoid Dendritic Cells, Metabolism Concepts, Monocyte-Derived, Role Concept, blood cells, Playthings and Play, chemical analysis, Protein, Systems, shelf, Phenomena, Role, Plaything, concluding remarks, bones, scales, Adults, Odontoclasts, metabolism, flange, organ process, Monocyte-Derived Macrophages, Metabolic Phenomenon, Macrophage, mobilization, multicellular organism metabolic process, osseous tissue, Plasmacytoid Dendritic Cell, adults, Peripheral Blood, scale, biodegradation, Veiled Cell, Metabolic, Macrophages, portion of bone tissue, shelves, System, Veiled, Interdigitating Cells, conclusion, Toys, signs, Bone Marrow Derived Macrophages, circulating cell, Odontoclast, Bones, projection, ridge, turnover, signalling, Reticuloendothelial System, Protein Gene Products, summary, process, processes, Gene Proteins, single-organism metabolic process, mineralized bone tissue, signalling process, bone, spine, Cells, Bone Marrow-Derived Macrophages, Interdigitating Dendritic Cells, processus, Interdigitating Cell, calcium tissue, assay, bone organ, Osseous, Proteomes, Bone, Anabolism5906http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210028860dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0project~1uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0181986428130783450.01.0falseCharacterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling.Data from ProteomeXchange, PXD ID: PXD000471. Experiment: 101207_EA_E, file: 101215_EA_E13.mzml. Published as part of . From the Abstract: {{i}} In addition to forming macrophages and dendritic cells, monocytes in adult peripheral blood retain the ability to develop into osteoclasts, mature bone-resorbing cells. The extensive morphological and functional transformation that occurs during osteoclast differentiation requires substantial reprogramming of gene and protein expression. Here we employ -omic scale technologies to examine in detail the molecular changes at discrete developmental stages in this process (precursor cells, intermediate osteoclasts and multinuclear osteoclasts), quantitatively comparing their transcriptomes and proteomes ... {{/i}}2014-07-23GPM1121002886025044017PXD000471PXD000471