{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":12,"searchCount":3},"additional":{"omics_type":["Other"],"submitter":["Jihye Shin, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Homo_sapiens_viruses, Cow,human"],"publication":["25216327"],"submitter_mail":["Not available"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029830","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029798","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029831","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029832","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029799","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029833","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029811","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029812","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029813","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029814","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029817","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029818","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029819","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029820","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029821","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029800","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029822","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029801","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029823","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029802","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029824","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029825","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029803","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029804","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029826","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029805","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029827","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029806","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029828","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029807","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029829","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029808","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210029809"],"submitter_affiliation":["Not available"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["To discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential to be secreted from tissues into circulation. A total of 898 human proteins were identified, of which 62.2% were predicted to be released or shed from cells. The identified proteins were compared with tissue proteomes to find candidate proteins whose expressions were elevated in tumor tissues compared with normal tissues as revealed by (i) quantitative proteomic analysis based on cICAT and mTRAQ or (ii) data mining of immunohistochemical images piled in Human Protein Atlas database. By applying various stringent criteria, 11 candidate proteins were selected. Among these, we validated an significant increase (p = 0.0018) of melanotransferrin (TRFM) at the plasma level of CRC patients through Western blotting, using 130 plasma samples containing 30 healthy controls, 80 CRC patients, and 20 patients of other diseases. Finally, we measured the expression level of TRFM in 325 plasma samples containing 77 healthy controls and 228 CRC patients (34.6 ± 4.2 ng/mL and 67.0 ± 6.4 ng/mL, p < 0.0001) through ELISA and demonstrated the area under the receiver operating characteristic curve of 0.723 (p < 0.0001) with a 92.5% specificity, 48.2% sensitivity, and 95.7% positive predictive value. Furthermore, unlike CEA and PAI-1, up-regulation of TRFM in pathological stages I & II groups compared with stages III & IV groups lead us to expect the use TRFM for early-stage diagnosis of CRC. In this study, we suggest TRFM as a potential serological marker for CRC and expect our discovery strategy to help identify highly cancer-specific and body-fluid-accessible biomarkers."],"pubmed_title":["Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics."],"pubmed_authors":["Shin Jihye J,Kim Hye-Jung HJ,Kim Gamin G,Song Meiying M,Woo Se Joon SJ,Lee Seung-Taek ST,Kim Hoguen H,Lee Cheolju C,","Shin Jihye J, Kim Hye-Jung HJ, Kim Gamin G, Song Meiying M, Woo Se Joon SJ, Lee Seung-Taek ST, Kim Hoguen H, Lee Cheolju C"],"name_synonyms":["MAP97, Cancer of Large Bowel, MFI2, Large Bowel Carcinoma, Colorectal Cancer, Large Intestine Cancer, determination, number, Cancer of Large Intestine, Cancers, Large Intestine Carcinoma, presence, MTf, membrane-bound transferrin-like protein p97, Colorectal, count in organism, presence or absence in organism., count, melanoma-associated antigen p97, chemical analysis, Carcinoma of Large Intestine, CRC, assay, Colorectal Cancers, quantitative, CD228, Malignant Colorectal Neoplasm, Large Bowel Cancer, Cancer, Carcinoma of Large Bowel"],"description_synonyms":["Gpi, data, Large Bowel Carcinoma, human being, Colorectal Cancer, AI461847, SCRIB1, Proteins, Cell Lines, Cancer of Large Intestine, Gene, cell_line, MF, Homo sapiense, Amf, Large Intestine Carcinoma, Cell, predicted, polypeptide, Colorectal, Homo spaiens, Homo sapien, mOC-X, Homo sapian, Homo sapians, Protein, Line, Gene Products, Carcinoma of Large Intestine, Pgi, Cell., NK|GPI, CRC, Crc, Colorectal Cancers, Homo sapeins, Gpi-1, NK, Large Bowel Cancer, Carcinoma of Large Bowel, Lines, Cancer of Large Bowel, Org, ORG, Homo sapients, HCT-116, Large Intestine Cancer, Gpi-1r, Nlk, Gpi-1s, Phi, Scrb1, Tissue, Gpi-1t, Humo sapiens, Gpi1-r, cell line cell, Gpi1-s, proteins, Cancers, CRIB, Gpi1-t, man, Homo sapines, cell line, human, Protein Gene Products, Gene Proteins, Homo spiens, \"human\" EXACT genbank_common_name [], Homo sapience, HCT 116, vartul, Homo sampiens, mKIAA0147, Malignant Colorectal Neoplasm, Home sapiens, Bglap-rs1, Gpi1s, AI118201, Cancer"],"pubmed_title_synonyms":["MAP97, Cancer of Large Bowel, MFI2, Large Bowel Carcinoma, Colorectal Cancer, Large Intestine Cancer, determination, number, Cancer of Large Intestine, Cancers, Large Intestine Carcinoma, presence, MTf, membrane-bound transferrin-like protein p97, Colorectal, count in organism, presence or absence in organism., count, melanoma-associated antigen p97, chemical analysis, Carcinoma of Large Intestine, CRC, assay, Colorectal Cancers, quantitative, CD228, Malignant Colorectal Neoplasm, Large Bowel Cancer, Cancer, Carcinoma of Large Bowel"],"pubmed_abstract_synonyms":["first cervical vertebra, Viral Marker, Biological Markers, Large Bowel Carcinoma, Antemortem Diagnosis, Surrogate Endpoints, Laboratory, Blood, Biochemical, Endpoint, Homo sapiense, Tumor, Serum, fluid, Large Intestine Carcinoma, Diagnosis, CEA, Cea, Laboratory Markers, Homo sapien, PSG1, CGM4AA, Biological, Cear, Homo sapians, Line, symptoms, CD228, animal, Large Bowel Cancer, average, portion of tissue, Fresh Frozen Plasmas, Fresh Frozen, Homo sapients, MFI2, Large Intestine Cancer, N, C1, Tissue, cell line cell, proteins, elevated, cell line, Immune, Markers, vertebra 1, Homo sapience, CD66e, Viral Markers, stage, PAI, Home sapiens, [Pb], plasma, Tumors, screening, Frozen Plasma, Viral, nanogram per millilitre, Endothelial plasminogen activator inhibitor, plomo, Surrogate Endpoint, Blei, Serpin E1, Up-Regulation (Physiology), Cancer of Large Intestine, RATCGM4AA, plomb, 82Pb, Biochemical Markers, Normalcy, PAI-1, Plasmas, Biologic Marker, PAI1, tumours, predicted, Diagnoses, organism, Postmortem, count, Homo sapian, Marker, Examinations and Diagnoses, Diseases, precocious, tissue portion, simple tissue, Colorectal Cancers, Postmortem Diagnosis, Carcinoma of Large Bowel, Lines, portion of blood plasma, Postmortem Diagnoses, Ceacam5, Cgm1, whole organism, End Points, Cgm4, Upregulation, signs, Immunologic, Laboratory Marker, Homo sapines, human, early, blood plasm, Health, melanoma-associated antigen p97, Patient, Specificity and Sensitivity, Biochemical Marker, Text Mining, RATCEAA, Koerper, atlas (CI), CGM2, mKIAA0147, Proteomes, AI118201, MAP97, Fresh, PLANH1, advanced, atlas, human being, multi-cellular organism, Colorectal Cancer, Fresh Frozen Plasma, Clinical Markers, Clinical Marker, Neoplasms, developmental stage, Mining, number, lead atom, Gene, InChI=1/Pb, presence, NEOPL, Surrogate End Points, Colorectal, Surrogate Markers, Homo spaiens, PSBG2, Gene Products, body fluid, Carcinoma of Large Intestine, Antemortem, cervical atlas, sensitivity, malignant tumour, Meconium antigen 100, Normalities, study, Cancer of Large Bowel, Blood Plasma, Clinical, Receptor Up-Regulation, Up Regulation, Biological Marker, Scrb1, malignant neoplasia, Psg16, tumour, Diagnoses and Examinations, man, membrane-bound transferrin-like protein p97, MALIGNANT AND UNSPECIFIED (INCL CYSTS AND POLYPS), Immunologic Markers, Text, vartul, Homo sampiens, Clients, Blood Plasmas, Planh1, Sensitivity, high elevation, atlas vertebra, Immunologic Marker, Neoplastic Growth, Biologic, Cancer, atlas [C I], Antemortem Diagnoses, use, findings, ng/ml, Malignant Neoplasm, body, SCRIB1, Proteins, Serum Markers, Cell Lines, End Point, whole body, cell_line, Client, Cell, MTf, Immune Marker, polypeptide, Frozen Plasmas, count in organism, Surrogate End Point, Protein, proteomic analysis, CRC, Crc, Homo sapeins, Viral., Data Base, ELISA, Biologic Markers, Plasma, Serum Marker, cervical vertebra 1, Normality, Surrogate, plumbum, Endpoints, Carcinoembryonic antigen, Specificity, Humo sapiens, portion of plasma, Cancers, CRIB, malignant tumor, Surrogate Marker, Protein Gene Products, Gene Proteins, Homo spiens, \"human\" EXACT genbank_common_name [], CEA5, InChIKey=WABPQHHGFIMREM-UHFFFAOYAT, Data, CEA4, quantitative, Malignant Colorectal Neoplasm, NEOPLASMS BENIGN, Neoplasia, C1 vertebra, presence or absence in organism, Immune 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of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.","description":"Data from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00565 PASS00565]]. File: 100521_HCT-116_OG11.mzml. Published as part of  . From the Abstract: {{i}} In order to discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential to be secreted from tissues into circulation. A total of 898 human proteins were identified, of which 62.2% were predicted to be released or shed from cells.{{/i}}","dates":{"submission":"2014-09-19"},"accession":"GPM11210029831","cross_references":{"pubmed":["25216327"]}}