GPMDB00315OtherShender VO, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanvictoria.shender@gmail.com25271300http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030789http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030794http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030795http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030781http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030792http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030793http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030787http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030788http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030796http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030786http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030797http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030790http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030791Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Federation, et al.Not availableGPMDBOvarian cancer ascites is a native medium for cancer cells that allows investigation of their secretome in a natural environment. This medium is of interest as a promising source of potential biomarkers, and also as a medium for cell-cell communication. The aim of this study was to elucidate specific features of the malignant ascites metabolome and proteome. In order to omit components of the systemic response to ascites formation, we compared malignant ascites with cirrhosis ascites. Metabolome analysis revealed 41 components that differed significantly between malignant and cirrhosis ascites. Most of the identified cancer-specific metabolites are known to be important signaling molecules. Proteomic analysis identified 2096 and 1855 proteins in the ovarian cancer and cirrhosis ascites, respectively; 424 proteins were specific for the malignant ascites. Functional analysis of the proteome demonstrated that the major differences between cirrhosis and malignant ascites were observed for the cluster of spliceosomal proteins. Additionally, we demonstrate that several splicing RNAs were exclusively detected in malignant ascites, where they probably existed within protein complexes. This result was confirmed in vitro using an ovarian cancer cell line. Identification of spliceosomal proteins and RNAs in an extracellular medium is of particular interest; the finding suggests that they might play a role in the communication between cancer cells. In addition, malignant ascites contains a high number of exosomes that are known to play an important role in signal transduction. Thus our study reveals the specific features of malignant ascites that are associated with its function as a medium of intercellular communication.Proteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication.Shender Victoria O VO,Pavlyukov Marat S MS,Ziganshin Rustam H RH,Arapidi Georgij P GP,Kovalchuk Sergey I SI,Anikanov Nikolay A NA,Altukhov Ilya A IA,Alexeev Dmitry G DG,Butenko Ivan O IO,Shavarda Alexey L AL,Khomyakova Elena B EB,Evtushenko Evgeniy E,Ashrafyan Lev A LA,Antonova Irina B IB,Kuznetcov Igor N IN,Gorbachev Alexey Yu AY,Shakhparonov Mikhail I MI,Govorun Vadim M VM,Shender Victoria O VO, Pavlyukov Marat S MS, Ziganshin Rustam H RH, Arapidi Georgij P GP, Kovalchuk Sergey I SI, Anikanov Nikolay A NA, Altukhov Ilya A IA, Alexeev Dmitry G DG, Butenko Ivan O IO, Shavarda Alexey L AL, Khomyakova Elena B EB, Evtushenko Evgeniy E, Ashrafyan Lev A LA, Antonova Irina B IB, Kuznetcov Igor N IN, Gorbachev Alexey Yu AY, Shakhparonov Mikhail I MI, Govorun Vadim M VMascites fluid, Personal Communication, Ovary Cancer, Cancer of the Ovary, Misinformation, OVARY NEOPL, Cancer of Ovary, Metabolic, Metabolomes, Personnel, Profile, Neoplasms, Ovarian Cancer, Ovary, OC, ascites, Ovary Neoplasms, Profiles, Cancers, Ovary Cancers, Ovarian Neoplasms, Communication, NEOPL OVARIAN, Programs, Communication Programs, OVARIAN NEOPL, Program, Personal, Ovarian Neoplasm, Ovarian, Communications Personnel, Neoplasm, Ovarian Cancers, ascitic fluid, Communication Program, Communications., Metabolic Profile, Proteomes, Metabolic Profiles, Cancer, Ovary NeoplasmOvary Cancer, biological signaling, PLXN5, HSN1E, determination, mol, Slf, Neoplasms, Somatomedin-C, Nl1, EA1, ascites, Gene, FPH2, PLEXIN-B1, Profiles, biosynthesis, MLR-3, Cirrhosis of liver, SeP, Hepatic Cirrhoses, OVARIAN NEOPL, AIM, Cirrhosis of liver without mention of alcohol, CEH, Ovarian, responsivity, CIRRH, Gene Products, NEPII, NL1, Fibroses, NL2, Cirrhoses, SEH, early activation antigen CD69, Metabolic Profile, malignant tumour, multicellular organismal biosynthetic process, SEP, Metabolic Profiles, study, reactivity, single-organism biosynthetic process, OVARY NEOPL, formation, cell, activation inducer molecule, Metabolomes, anabolism, Profile, Ovarian Cancer, Liver Fibrosis, somatomedin-C, SF, malignant neoplasia, Kitl, proteins, Mast cell growth factor, sEP, ascitic fluid., synthesis, NEOPL OVARIAN, leukocyte surface antigen Leu-23, Liver Cirrhoses, IGF1, signaling process, Mechano growth factor, SELP, C-type lectin domain family 2 member C, Hepatic, Soluble KIT ligand, ascitic fluid, Ovarian Cancers, Sl, single organism signaling, Cancer, ascites fluid, IGF-I, data, Cancer of Ovary, steel factor, Liver, Malignant Neoplasm, hematopoietic growth factor KL, Proteins, DNMT, Ovary, somatomedin, Mell1, Cirrhosis of liver NOS (disorder), MCMT, Ovary Cancers, Cell, sKITLG, BL-AC/P26, polypeptide, MMEL2, mechano growth factor, liver cirrhosis, chemical analysis, Protein, proteomic analysis, Neoplasm, Cirrhosis, NOS, Fibrosis, Igf-1, Ovary Neoplasm, CXXC9, early T-cell activation antigen p60, Cirrhosis of liver NOS, Cancer of the Ovary, ADCADN, SHEP7, mast cell growth factor, Metabolic, Stem cell factor, OC, Liver Fibroses, Ovary Neoplasms, Cancers, STAT5, Ovarian Neoplasms, malignant tumor, cirrhosis, signalling, Protein Gene Products, Gene Proteins, stem cell factor, KL-1, signalling process, Ovarian Neoplasm, GP32/28, CL - Cirrhosis of liver, MGF, c-Kit ligand, KITLG, Eph2, Hepatic cirrhosis, assay, response, SCF, Cirrhosis of liver (disorder), CD69, Proteomes, CLEC2C, NEP2ascites fluid, Personal Communication, Ovary Cancer, Cancer of the Ovary, Misinformation, OVARY NEOPL, Cancer of Ovary, Metabolic, Metabolomes, Personnel, Profile, Neoplasms, Ovarian Cancer, Ovary, OC, ascites, Ovary Neoplasms, Profiles, Cancers, Ovary Cancers, Ovarian Neoplasms, Communication, NEOPL OVARIAN, Programs, Communication Programs, OVARIAN NEOPL, Program, Personal, Ovarian Neoplasm, Ovarian, Communications Personnel, Neoplasm, Ovarian Cancers, ascitic fluid, Communication Program, Communications., Metabolic Profile, Proteomes, Metabolic Profiles, Cancer, Ovary NeoplasmSignal Transductions, Viral Marker, Biological Markers, Surrogate Endpoints, HSN1E, determination, Laboratory, nutrient medium, Biochemical, ascites, Endpoint, via spliceosome, Profiles, Serum, Cirrhosis of liver, Cell Interactions, Hepatic Cirrhoses, Receptor Mediated Signal Transduction, Laboratory Markers, Personal, Communications, Roles, Biological, responsivity, CIRRH, Concepts, Impacts, Cirrhoses, Cell-to-Cell, Environmental Impacts, multicellular organismal biosynthetic process, Metabolic Profiles, OVARY NEOPL, single-organism biosynthetic process, Identification, activation inducer molecule, Personnel, Cell to Cell Interaction, anabolism, Liver Fibrosis, Pathways, proteins, number of, Communication, NEOPL OVARIAN, Environmental Impact, Immune, Markers, Liver Cirrhoses, Viral Markers, signaling process, Role Concepts, Signal Transduction Pathways, Ovarian Cancers, ascitic fluid, Transductions, associated, single organism signaling, Diagnostic Findings, ascites fluid, SIGNS SYMPTOMS, Cancer of Ovary, Liver, Pathway, Viral, Surrogate Endpoint, DESC, Biochemical Markers, extra or missing physical or functional parts, Biologic Marker, Programs, Program, signaling cascade, Interaction, Role Concept, Playthings and Play, Marker, Role, Cirrhosis, Plaything, NOS, Fibrosis, Transduction, Ovary Neoplasm, early T-cell activation antigen p60, Cirrhosis of liver NOS, ADCADN, Cell Communications, End Points, medium, System, Toys, Identifications (Psychology), Liver Fibroses, Ovary Neoplasms, Immunologic, Laboratory Marker, signaling pathway, Biochemical Marker, Communication Program, Cirrhosis of liver (disorder), ovarian carcinoma cell line, Clinical Finding, CD69, Proteomes, Ovary Cancer, Misinformation, Signal Transduction Systems, biological signaling, Plays, nuclear mRNA cis splicing, Clinical Markers, Clinical Marker, Neoplasms, number, Receptor-Mediated, EA1, Gene, Signal Transduction System, biosynthesis, MLR-3, splicing, Symptoms and Signs, Surrogate End Points, OVARIAN NEOPL, Surrogate Markers, Signal Transduction, AIM, Cirrhosis of liver without mention of alcohol, Ovarian, Cell-to-Cell Interactions, Gene Products, Fibroses, Finding, early activation antigen CD69, Communications., Metabolic Profile, malignant tumour, Toy, study, reactivity, Clinical, ovarian cancer cell line, Cell Interaction, Playthings, formation, cell, Metabolomes, Profile, Biological Marker, Ovarian Cancer, Signal, malignant neoplasia, Cell-to-Cell Interaction, Signal Pathways, Signal Transduction Pathway, synthesis, Communication Programs, via U2-type spliceosome, signalling pathway, Immunologic Markers, leukocyte surface antigen Leu-23, Puppets, has or lacks parts of type, C-type lectin domain family 2 member C, Play, Hepatic, Immunologic Marker, Signal Pathway, Biologic, Puppet, Cancer, Personal Communication, Malignant Neoplasm, Serum Markers, Proteins, Ovary, DNMT, End Point, Receptor-Mediated Signal Transductions, function, Cirrhosis of liver NOS (disorder), MCMT, Ovary Cancers, Cell, Immune Marker, Impact, Concept, mereological quality, Environmental, BL-AC/P26, polypeptide, Signs and Symptoms, Surrogate End Point, Receptor-Mediated Signal Transduction, liver cirrhosis, chemical analysis, Protein, Communications Personnel, Systems, proteomic analysis, Neoplasm, signalling cascade, Biologic Markers, CXXC9, Cancer of the Ovary, Serum Marker, Metabolic, growth medium, Surrogate, Endpoints, OC, Cancers, Ovarian Neoplasms, malignant tumor, Surrogate Marker, cirrhosis, signalling, Protein Gene Products, Gene Proteins, extracellular, Ovarian Neoplasm, signalling process, GP32/28, CL - Cirrhosis of liver, cardinality, Environments, Hepatic cirrhosis, assay, response, Interactions, CLEC2C, Immune Markers3105http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210030783dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0095619987661937080.01.0falseProteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication.Data from ProteomeXchange, PXD ID: PXD001403. File: V022-V023+V053_line8_MGFPeaklist.mgf. Published as part of Mol Cell Proteomics. 2014 Sep 30 . From the Abstract: {{i}} The aim of this study was to elucidate specific features of malignant ascites metabolome and proteome. To omit components that belong to systemic response to the ascites formation we compared malignant ascites with cirrhosis one. Metabolome analysis revealed 41 components that differed significantly between malignant and cirrhosis ascites. Most of the identified cancer-specific metabolites are known to be important signaling molecules. Proteomic analysis identified 2096 and 1855 proteins in the ovarian cancer and cirrhosis ascites, respectively, 424 proteins were specific for the malignant ascites. {{/i}}2014-10-27GPM1121003078325271300PXD001403PXD001403