GPMDB00305OtherCarney KE, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mousemark.verheijen@vu.nl25308431http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031419http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031418http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031417http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031416http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031411http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031410http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031399http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031398http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031397http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031415http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031414http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031413http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031412http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031391http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031390http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031396http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031395http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031394http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031393http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031408http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031407http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031406http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031405http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031409http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031400http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031389http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031422http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031388http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031421http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031420http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031387http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031386http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031426http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031404http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031403http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031425http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031402http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031424http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031401http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031423http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031385Department of Molecular and Cellular Neurobiology, VU University AmsterdamCL:0000127 astrocyteGPMDBAstrocytes are being increasingly recognized as crucial contributors to neuronal function at synapses, axons, and somas. Reliable methods that can provide insight into astrocyte proteins at the neuron-astrocyte functional interface are highly desirable. Here, we conducted a mass spectrometry analysis of Percoll gradient-isolated gliosomes, a viable preparation of glial subcellular particles often used to study mechanisms of astrocytic transmitter uptake and release and their regulation. Gliosomes were compared with synaptosomes, a preparation containing the neurotransmitter release machinery, and, accordingly, synaptosomes were enriched for proteins involved in synaptic vesicle-mediated transport. Interestingly, gliosome preparations were found to be enriched for different classes of known astrocyte proteins, such as VAMP3 (involved in astrocyte exocytosis), Ezrin (perisynaptic astrocyte cytoskeletal protein), and Basigin (astrocyte membrane glycoprotein), as well as for G-protein-mediated signaling proteins. Mass spectrometry data are available via ProteomeXchange with the identifier PXD001375. Together, these data provide the first detailed description of the gliosome proteome and show that gliosomes can be a useful preparation to study glial membrane proteins and associated processes.Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins.Carney Karen E KE,Milanese Marco M,van Nierop Pim P,Li Ka Wan KW,Oliet Stéphane H R SH,Smit August B AB,Bonanno Giambattista G,Verheijen Mark H G MH,Carney Karen E KE, Milanese Marco M, van Nierop Pim P, Li Ka Wan KW, Oliet Stéphane H R SH, Smit August B AB, Bonanno Giambattista G, Verheijen Mark H G MHthe brain, Brain, Integral Membrane Proteins, 3.4.22.-, suprasegmental structures, Entire brain, Laboratory, Mus domesticus, Proteins, Surface Proteins, mouse, Cell Surface, mini-ICE, CASP-14, Membrane-Associated Proteins, Integral, Encephalons, House Mouse, encephalon, brain structure, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, House, Mus, Mini-ICE, Protein, proteomic analysis, central nervous system, Membrane Proteins, Mus musculus domesticus, Integral., synganglion, Mice, Cell Surface Proteins, Cell Membrane, Mus musculus, study, Nervous System, Caspase-14 subunit p10, CNS, Brains, Identification, Swiss, mice, Encephalon, Surface, Membrane-Associated, Swiss Mouse, Identifications (Psychology), House Mice, Swiss Mice, Caspase-14 subunit p19, Cell Membrane Proteins, suprasegmental levels of nervous system, Membrane, "mouse" EXACT common_name [], MICE, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], Integral Membrane Protein, Membrane Associated Proteins, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, house mouse, Membrane Protein, Laboratory Mouse, Integral MembraneIGF-I, data, astrocytic glia, steel factor, nerve cell, Procedures, Slf, protein sorting along secretory pathway, hematopoietic growth factor KL, AUTSX5, Proteins, Somatomedin-C, Synaptosome, somatomedin, DXS648, Gene, FPH2, function, Procedure, vesicle trafficking., CCN3, QM, sKITLG, vesicular transport, polypeptide, uptake, Experiment, IGFBP-9, neuron cell, vesicle transport, Astrocyte, Method, mechano growth factor, Astroglias, Protein, Studies, Gene Products, Synapse, NOV, techniques, Igf-1, PlexA1, study, neurotransmitter secretory pathway, SHEP7, Plxn1, nonselective vesicle transport, mast cell growth factor, neurotransmitter release, Stem cell factor, somatomedin-C, SF, Kitl, proteins, Methodological, procedures, IGFBP9, STAT5, Methodological Study, Mast cell growth factor, mKIAA4053, IBP-9, Protein Gene Products, Study, Gene Proteins, stem cell factor, KL-1, neuronal cell, Methodological Studies, Axon, L10, IGF1, C130088N23Rik, MGF, c-Kit ligand, Mechano growth factor, KITLG, 2600013D04Rik, Astroglia, regulation, Soluble KIT ligand, SCF, DXS648E, NOVh, Sl, Proteomes, methodologythe brain, Brain, Integral Membrane Proteins, 3.4.22.-, suprasegmental structures, Entire brain, Laboratory, Mus domesticus, Proteins, Surface Proteins, mouse, Cell Surface, mini-ICE, CASP-14, Membrane-Associated Proteins, Integral, Encephalons, House Mouse, encephalon, brain structure, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, House, Mus, Mini-ICE, Protein, proteomic analysis, central nervous system, Membrane Proteins, Mus musculus domesticus, Integral., synganglion, Mice, Cell Surface Proteins, Cell Membrane, Mus musculus, study, Nervous System, Caspase-14 subunit p10, CNS, Brains, Identification, Swiss, mice, Encephalon, Surface, Membrane-Associated, Swiss Mouse, Identifications (Psychology), House Mice, Swiss Mice, Caspase-14 subunit p19, Cell Membrane Proteins, suprasegmental levels of nervous system, Membrane, "mouse" EXACT common_name [], MICE, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], Integral Membrane Protein, Membrane Associated Proteins, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, house mouse, Membrane Protein, Laboratory Mouse, Integral Membraneprojections, Integral Membrane Proteins, biological signaling, nerve cell, Procedures, lamellae, protein sorting along secretory pathway, Surface Proteins, Synaptosome, Gene, CD147 Antigens, Membrane-Associated Proteins, Spectrum Analyses, Integral, organ process., D130027G05Rik, process of organ, protrusion, CEB, CD147 Antigen, lamella, rat, vesicle transport, Method, Basigin Protein, Studies, Gene Products, Antigen, Mass, Synapse, Vil2 protein, Analysis, villin 2 protein, Mass Spectroscopy, vesicle trafficking, Mass Spectrum Analysis, ceb, Cell Membrane, study, neurotransmitter secretory pathway, Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), Analyses, neurotransmitter release, Surface, proteins, procedures, ridges, Membrane glycoprotein, Study, Methodological Studies, Axon, Emmprin Protein, signaling process, papilla, Membrane Associated Proteins, vesicle exocytosis, VIL2 protein, associated, cytovillin, laminae, Membrane Protein, nonselective vesicle exocytosis, single organism signaling, data, astrocytic glia, anatomical protrusion, Emmprin, anatomical process, Proteins, mouse, lamina, flanges, Cell Surface, function, Procedure, Spectrum Analysis, vesicular transport, Antigens, Spectroscopy, polypeptide, uptake, neuron cell, Astrocyte, Astroglias, Protein, shelf, Membrane Proteins, techniques, CD147, Mass Spectrum Analyses, Cell Surface Proteins, flange, Mass Spectrum, nonselective vesicle transport, VAMP-3, Protein Cd86, shelves, Membrane-Associated, Spectrometry, Cell Membrane Proteins, Methodological, phosphoprotein p81, Membrane, villin-2, Methodological Study, projection, villin 2 (ezrin) protein, ridge, human, signalling, Protein Gene Products, Gene Proteins, process, processes, Integral Membrane Protein, neuronal cell, signalling process, spine, processus, Astroglia, regulation, Proteomes, methodology, Integral Membrane3005http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210031417dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0092535471930906850.01.0falseProteomic Analysis of Gliosomes from Mouse Brain: Identification and Investigation of Glial Membrane Proteins.Data from ProteomeXchange, PXD ID: PXD001375. Experiment: Synaptosome, file: g.Synaptosome_s.4_p.4_x.1.wiff_centroid.mgf. Published as part of J Proteome Res. 2014 Nov 4 . From the Abstract: {{i}} Astrocytes are being increasingly recognized as crucial contributors to neuronal function at synapses, axons, and somas. Reliable methods that can provide insight into astrocyte proteins at the neuron-astrocyte functional interface are highly desirable. Here, we conducted a mass spectrometry analysis of Percoll gradient-isolated gliosomes, a viable preparation of glial subcellular particles often used to study mechanisms of astrocytic transmitter uptake and release and their regulation. Gliosomes were compared with synaptosomes, a preparation containing the neurotransmitter release machinery, and, accordingly, synaptosomes were enriched for proteins involved in synaptic vesicle-mediated transport ... {{/i}}2014-11-17GPM1121003141725308431PXD001375PXD001375