{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":27,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Sandin M, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Streptococcus_pyogenes_m1_gas, Streptococcus_pyogenes_manfredo"],"submitter_mail":["fredrik.levander@immun.lth.se"],"publication":["25656266"],"submitter_affiliation":["Department of Immunotechnology, Lund University"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037526"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label-free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future."],"pubmed_title":["Is label-free LC-MS/MS ready for biomarker discovery?"],"pubmed_authors":["Sandin Marianne M,Chawade Aakash A,Levander Fredrik F,","Sandin Marianne M, Chawade Aakash A, Levander Fredrik F"],"name_synonyms":["LC-MS2, liquid chromatography tandem mass spectroscopy, LC-MS/MS, liquid chromatography tandem mass spectrometry, LC/MS/MS, LCMSMS, biological marker., liquid chromatography-tandem mass spectroscopy, free, LC-MSMS, LC-MS-MS"],"description_synonyms":["data, DIP13alpha, Experiment, APPL."],"pubmed_title_synonyms":["LC-MS2, liquid chromatography tandem mass spectroscopy, LC-MS/MS, liquid chromatography tandem mass spectrometry, LC/MS/MS, LCMSMS, biological marker., liquid chromatography-tandem mass spectroscopy, free, LC-MSMS, LC-MS-MS"],"pubmed_abstract_synonyms":["liquid chromatography tandem mass spectroscopy, big, Viral Marker, Biological Markers, Labor Force, Surrogate Endpoints, Procedures, Activity, Laboratory, Clinical Markers, Clinical Marker, number, Biochemical, Endpoint, Employment Insecurity, Labor Forces, Serum, presence, supernumerary, Child, LC-MS-MS, Status, Surrogate End Points, Surrogate Markers, large, Laboratory Markers, Marginal, Method, Biological, LC-MSMS, Studies, Research Activity, Laboratory Research, Marginal Employment, Priorities, increased, PROS, LCMSMS, Occupational, Clinical, Research, Child Labor, Labor, Biological Marker, procedures, number of, Employment Status, free, Study, Immunologic Markers, Immune, Markers, Methodological Studies, Employment Insecurities, Viral Markers, sample, great, has or lacks parts of type, Insecurity, biological marker, Development and Research, Research Priority, Immunologic Marker, Biologic, Precarious, LC-MS2, use, Viral, THPH5, Surrogate Endpoint, THPH6, Serum Markers, free., LC-MS/MS, End Point, Research Priorities, Biochemical Markers, extra or missing physical or functional parts, Procedure, Biologic Marker, PSA, Immune Marker, PS24, Occupational Status, mereological quality, PS23, PS25, count in organism, LC/MS/MS, Priority, PS22, PS21, count, Marker, Surrogate End Point, Research Activities, techniques, Biologic Markers, Research and Development, Serum Marker, End Points, Precarious Employment, Surrogate, increased number, Employment Termination, Endpoints, expanded, Employment, Methodological, Immunologic, Laboratory Marker, Methodological Study, sample population, Surrogate Marker, Activities, present in greater numbers in organism, enlarged, Termination, Biochemical Marker, liquid chromatography-tandem mass spectroscopy, cardinality, Underemployment, liquid chromatography tandem mass spectrometry, quantitative, accessory, methodology, presence or absence in organism, Immune Markers"],"view_count":["27"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037526"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.008328192473781616"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Is label-free LC-MS/MS ready for biomarker discovery?","description":"Data from ProteomeXchange, PXD ID: PXD002037. Experiment: 01, file: folder summary. Published as part of Proteomics Clin Appl. 2015 Feb 5  .","dates":{"submission":"2015-04-20"},"accession":"GPM11210037526","cross_references":{"pubmed":["25656266"],"Pride":["PXD002037"],"pride":[],"Pride Archive":["PXD002037"]}}