GPMDB0055364OtherMarcone S, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humansimone.marcone@ucd.ie23606334School of Medicine and Medical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublinhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037759http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037757http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037758http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037755http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037756http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037753http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037754http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037751http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037752http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037750http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037748http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037749http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037746http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037747http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037744http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037745http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037742http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037743http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037740http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037741http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037785http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037780http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037781http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037739http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037737http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037738http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037779http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037735http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037736http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037777http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037733http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037734http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037731http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037775http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037776http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037732http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037773http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037774http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037730http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037771http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037772http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037770http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037728http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037729http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037726http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037727http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037768http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037724http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037769http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037725http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037766http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037767http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037764http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037765http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037762http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037763http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037760http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037761Not availableGPMDB15-Deoxy-delta12, 14-prostaglandin J(2) (15d-PGJ(2)) is an endogenous anti-inflammatory lipid derived from PGD(2). One potential mechanism for its activity is the covalent modification of cellular proteins, via a reactive α,β-unsaturated carbonyl group in its cyclopentenone ring, which in turn alters protein function. In order to identify the candidate target proteins covalently modified by 15d-PGJ(2) in human aortic endothelial cell (EC), EC was treated with biotinylated-15d-PGJ(2), the modified proteins extracted by Neutravidin affinity-purification and the proteins identified by LTQ Orbitrap mass spectrometer. Classification of the 358 identified proteins was performed using PANTHER classification system (www.pantherdb.org), showing that the proteins mapped to metabolic process, cellular process, and transport activity. This protein data set highlights the potential for 15d-PGJ(2) to covalently modify cellular proteins and provides a source of data that will aid further studies on the mechanism of action of this endogenous regulator of inflammation.Proteomic identification of the candidate target proteins of 15-deoxy-delta12,14-prostaglandin J2.Marcone Simone S,Fitzgerald Desmond J DJ,Marcone Simone S, Fitzgerald Desmond J DJProtein Gene Products, Gene Products, Proteins, Gene., Gene Proteins, polypeptide, Identifications (Psychology), proteins, Identification, ProteinProtein Gene Products, Gene Products, Proteins, Gene., Gene Proteins, polypeptide, data, Gene, HAEC cell, Experiment, proteins, ProteinProtein Gene Products, Gene Products, Proteins, Gene., Gene Proteins, polypeptide, Identifications (Psychology), proteins, Identification, ProteinHEL-S-113, Gpi, data, cell physiology, Inflammations, Data Set, PHGDHD, taxonomy, AI461847, metabolism resulting in cell growth, Proteins, 2-cyclopenten-1-one, Systematics, Gene, function, single-organism cellular process, MF, 3PGDH, Amf, body system, AID, Aid, PGDH, A10, polypeptide, Classifications, Taxonomies, solute:solute exchange, hierarchies, SERA, hierarchy, mOC-X, single-organism transport, PGD, systematics, CDA2, 6PGD, Protein, cellular projection, Gene Products, 3-PGDH, system, Pgi, HAEC cell, NK|GPI, connected anatomical system, Gpi-1, regulator, metabolism, NK, aid, multicellular organism metabolic process, cellular process, Taxonomy, Org, ORG, anatomical systems, inflammatory response, Lipids, cell process, Gpi-1r, Nlk, Arp2, Gpi-1s, ARP2, Phi, PDG, Gpi-1t, Inflammation., NLS, Gpi1-r, Gpi1-s, inflammation, proteins, metabolic process resulting in cell growth, 4930479N23, NLS1, Gpi1-t, Protein Gene Products, organ system, Gene Proteins, single-organism metabolic process, cell growth and/or maintenance, HEL-S-284, small molecule transport, HIGM2, cellular physiological process, Bglap-rs1, Gpi1s, PGAD550364http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210037732dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0intact~359pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0169648365206662541.00.0falseProteomic identification of the candidate target proteins of 15-deoxy-delta12,14-prostaglandin J2.Data from ProteomeXchange, PXD ID: PXD000006. Experiment: 1, file: 090712_1.8.mzml. Published as part of Proteomics. 2013 Jul;13(14):2135-9 . From the Abstract: {i}} ... In order to identify the candidate target proteins covalently modified by 15d-PGJ(2) in human aortic endothelial cell (EC), EC was treated with biotinylated-15d-PGJ(2), the modified proteins extracted by Neutravidin affinity-purification and the proteins identified by LTQ Orbitrap mass spectrometer ... {{/i}}2015-05-05GPM1121003773223606334PXD000006PXD000006