GPMDB00165OtherWisniewski JR, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanjwisniew@biochem.mpg.de26245529Max-Planck Institute of Biochemistry, Martinsried, Germanyhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040770http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040771http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040772http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040773http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040774http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040775http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040776http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040818http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040819http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040810http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040777http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040778http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040811http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040779http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040812http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040813http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040814http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040815http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040816http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040817http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040780http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040820http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040787http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040829http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040821http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040788http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040822http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040789http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040823http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040824http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040825http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040826http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040827http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040828http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040791http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040792http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040793http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040794http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040795http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040796http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040830http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040797http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040798http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040790http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040799http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040764http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040765http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040807http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040808http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040809http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040766http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040800http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040767http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040801http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040802http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040769http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040803http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040804http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040805http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040806Not availableGPMDBColorectal cancer is a leading cause of cancer-related death. It develops from normal enterocytes, through a benign adenoma stage, into the cancer and finally into the metastatic form. We previously compared the proteomes of normal colorectal enterocytes, cancer and nodal metastasis to a depth of 8100 proteins and found extensive quantitative remodeling between normal and cancer tissues but not cancer and metastasis (Wiśniewski et al. PMID 22968445). Here we utilize advances in the proteomic workflow to perform an in depth analysis of the normal tissue (N), the adenoma (A), and the cancer (C). Absolute proteomics of 10 000 proteins per patient from microdissected formalin-fixed and paraffin-embedded clinical material established a quantitative protein repository of the disease. Between N and A, 23% of all proteins changed significantly, 17.8% from A to C and 21.6% from N to C. Together with principal component analysis of the patient groups, this suggests that N, A, and C are equidistant but not on one developmental line. Our proteomics approach allowed us to assess changes in varied cell size, the composition of different subcellular components, and alterations in basic biological processes including the energy metabolism, plasma membrane transport, DNA replication, and transcription. This revealed several-fold higher concentrations of enzymes in fatty acid metabolism in C compared with N, and unexpectedly, the same held true of plasma membrane transporters.Absolute Proteome Analysis of Colorectal Mucosa, Adenoma, and Cancer Reveals Drastic Changes in Fatty Acid Metabolism and Plasma Membrane Transporters.Wiśniewski Jacek R JR,Duś-Szachniewicz Kamila K,Ostasiewicz Paweł P,Ziółkowski Piotr P,Rakus Dariusz D,Mann Matthias M,Wiśniewski Jacek R JR, Duś-Szachniewicz Kamila K, Ostasiewicz Paweł P, Ziółkowski Piotr P, Rakus Dariusz D, Rakus Dariusz D, Mann Matthias Madenomas, Papillary Adenomas, Follicular Adenoma, inner endospore membrane., Microcystic Adenomas, cell membrane, Malignant Neoplasm, determination, Trabecular Adenomas, Adenomas, Adenoma, Basal Cell, Monomorphic Adenoma, malignant neoplasia, Cancers, Microcystic Adenoma, malignant tumor, Microcystic, Monomorphic Adenomas, Papillary, cellular membrane, plasma membrane lipid bilayer, fatty acid metabolism, Monomorphic, juxtamembrane, Papillary Adenoma, plasmalemma, chemical analysis, Trabecular, Basal Cell Adenoma, Follicular Adenomas, Trabecular Adenoma, assay, bacterial inner membrane, Basal Cell Adenomas, cytoplasmic membrane, Proteomes, Follicular, malignant tumour, CancerPapillary Adenomas, deceased, data, Large Bowel Carcinoma, Colorectal Cancer, Malignant Neoplasm, Trabecular Adenomas, determination, Cancer., Adenomas, Metastasis, Basal Cell, developmental stage, Metastases, Proteins, number, Cancer of Large Intestine, Gene, Neoplasm Metastases, Cardiac Death, Normalcy, Large Intestine Carcinoma, presence, Microcystic, Monomorphic Adenomas, polypeptide, Colorectal, count in organism, Workflows, Experiment, Cardiac, Tg.413d, Monomorphic, count, Protein, chemical analysis, post-mortem, Trabecular, Neoplasm, Gene Products, tissue portion, Carcinoma of Large Intestine, Near-Death Experience, simple tissue, CRC, Colorectal Cancers, Work Flow, Death, malignant tumour, Follicular, Large Bowel Cancer, Carcinoma of Large Bowel, Normalities, adenomas, average, portion of tissue, Cancer of Large Bowel, death, Follicular Adenoma, Microcystic Adenomas, Large Intestine Cancer, Determination of Death, Normality, Adenoma, HTX5, N, Monomorphic Adenoma, Tissue, malignant neoplasia, proteins, Cancers, benign, Microcystic Adenoma, malignant tumor, Protein Gene Products, Papillary, Gene Proteins, metastatic, Health, Papillary Adenoma, Basal Cell Adenoma, Follicular Adenomas, Trabecular Adenoma, stage, assay, quantitative, Basal Cell Adenomas, Malignant Colorectal Neoplasm, Enterocyte, Proteomes, Work Flows, NODAL, Cancer, presence or absence in organismadenomas, Papillary Adenomas, Follicular Adenoma, inner endospore membrane., Microcystic Adenomas, cell membrane, Malignant Neoplasm, determination, Trabecular Adenomas, Adenomas, Adenoma, Basal Cell, Monomorphic Adenoma, malignant neoplasia, Cancers, Microcystic Adenoma, malignant tumor, Microcystic, Monomorphic Adenomas, Papillary, cellular membrane, plasma membrane lipid bilayer, fatty acid metabolism, Monomorphic, juxtamembrane, Papillary Adenoma, plasmalemma, chemical analysis, Trabecular, Basal Cell Adenoma, Follicular Adenomas, Trabecular Adenoma, assay, bacterial inner membrane, Basal Cell Adenomas, cytoplasmic membrane, Proteomes, Follicular, malignant tumour, Cancerprojections, [H]C([H])=O, deceased, Formol, Large Bowel Carcinoma, Size, determination, Adenomas, Metastasis, Ass-1, Metabolisms, Bioenergetics, Neoplasm Metastases, Cardiac Death, Principal Component Analyses, Large Intestine Carcinoma, composed of, P270, Monomorphic Adenomas, actin-dependent regulator of chromatin subfamily F member 1, BAF250a, Methanal, Parafilm, AA408052, SWI|SNF-related, fold, Volume, Analysis, Autonomous Replication, Expenditure, Work Flow, Follicular, Large Bowel Cancer, adenomas, average, portion of tissue, human disease, Follicular Adenoma, Large Intestine Cancer, enzymes, Analyses, Adenoma, N, Tissue, Formaldehyd, Oxomethane, composition, proteins, benign, Microcystic Adenoma, Autonomous, InChI=1/CH2O/c1-2/h1H2, ASS, DNA-dependent, plasma membrane lipid bilayer, plasmalemma, papilla, Concentrations, Basal Cell Adenoma, Homo sapiens disease, stage, Basal Cell Adenomas, Enterocyte, ELD, NODAL, size of cell, Papillary Adenomas, hOSA1, anatomical protrusion, DNA-dependent transcription, lamina, Osa homolog 1, Cancer of Large Intestine, Volumes, flanges, Normalcy, cellular membrane, Workflows, juxtamembrane, count, Diseases, shelf, tissue portion, Sizes, Near-Death Experience, B120, simple tissue, bacterial inner membrane, Colorectal Cancers, fixed, Carcinoma of Large Bowel, cell membrane, Determination of Death, HTX5, content, shelves, OSA1, projection, ridge, Papillary, metastatic, Health, Energy Metabolisms, Principal Component, Replications, spine, Follicular Adenomas, Trabecular Adenoma, Autonomous Replications, DNA, Proteomes, Work Flows, transcription, Colorectal Cancer, InChIKey=WSFSSNUMVMOOMR-UHFFFAOYAT, Trabecular Adenomas, lamellae, Biocatalysts, developmental stage, number, Cell Volume, Gene, presence, process of organ, Microcystic, protrusion, lamella, Colorectal, hELD, Cardiac, Monomorphic, Metabolism, SWI-like protein, Gene Products, Carcinoma of Large Intestine, transcription regulator activity, membrane transport, DNA Replications, malignant tumour, Normalities, Cancer of Large Bowel, Microcystic Adenomas, BAF250, malignant neoplasia, Energy, ridges, Expenditures, Formalin, Concentration, BRG1-associated factor 250, Cell Volumes, laminae, Cancer, cellular transcription, inner endospore membrane., SMARCF1, Malignant Neoplasm, BM029, Basal Cell, anatomical process, Metastases, Proteins, enzyme activity, compositionality, CH2O, Cell, polypeptide, count in organism, Tg.413d, Protein, chemical analysis, post-mortem, Trabecular, Neoplasm, FORMALIN, CRC, Death, Replication, flange, organ process, Energy Expenditure, MRD14, BAF250A, death, C1orf4, Normality, Monomorphic Adenoma, Energy Expenditures, patient, Cancers, SWI|SNF complex protein p270, malignant tumor, Methylene oxide, Bioenergetic, Protein Gene Products, Gene Proteins, Cell Sizes, process, processes, matrix-associated, fatty acid metabolism, ARID domain-containing protein 1A, BRG1-associated factor 250a, Oxomethylene, Papillary Adenoma, structure, processus, assay, quantitative, cytoplasmic membrane, Malignant Colorectal Neoplasm, Attentions, presence or absence in organism1605http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210040827dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0049352251696483650.01.0falseAbsolute proteome analysis of colorectal mucosa, adenoma and cancer reveals drastic changes in fatty acid metabolism and plasma membrane transportersData from ProteomeXchange, PXD ID: PXD002137. Experiment: NOR9_T, file: 20120109_EXQ6_JRW_SA_NOR9_T_05.mzml. Published as part of J Proteome Res. 2015 Aug 6 . From the Abstract: {{i}} Colorectal cancer is a leading cause of cancer-related death. It develops from normal enterocytes, through a benign adenoma stage, into the cancer and finally into the metastatic form. We previously compared the proteomes of normal colorectal enterocytes (N), cancer (C) and nodal metastasis (M) to a depth of 8,100 proteins and found extensive quantitative remodeling between normal and cancer but not cancer and metastasis ... Here we utilize advances in the proteomic workflow to perform an in depth analysis of the normal tissue (N), the adenoma (A) and the cancer (C). {{/i}}2015-08-15GPM1121004082726245529PXD002137PXD002137