{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":62,"searchCount":7},"additional":{"omics_type":["Other"],"submitter":["Castello A, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Homo_sapiens_viruses, Human_female"],"submitter_mail":["jeroen.krijgsveld@embl.de"],"publication":["22658674"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210041750","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210041763","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210041776","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210041724","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210041737"],"submitter_affiliation":["European Molecular Biology Laboratory, Heidelberg"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed \"interactome capture,\" to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings."],"pubmed_title":["Insights into RNA biology from an atlas of mammalian mRNA-binding proteins."],"pubmed_authors":["Castello Alfredo A,Fischer Bernd B,Eichelbaum Katrin K,Horos Rastislav R,Beckmann Benedikt M BM,Strein Claudia C,Davey Norman E NE,Humphreys David T DT,Preiss Thomas T,Steinmetz Lars M LM,Krijgsveld Jeroen J,Hentze Matthias W MW,","Castello Alfredo A, Fischer Bernd B, Eichelbaum Katrin K, Horos Rastislav R, Beckmann Benedikt M BM, Strein Claudia C, Davey Norman E NE, Humphreys David T DT, Preiss Thomas T, Steinmetz Lars M LM, Krijgsveld Jeroen J, Hentze Matthias W MW"],"name_synonyms":["Gene., ribonucleic acid, Acid, RNA, Ribonucleic, first cervical vertebra, atlas, protein_coding_transcript, cervical vertebra 1, mRNA, ribose nucleic acid, Non Polyadenylated RNA, C1, ligand, Proteins, ribonucleic acids, Non-Polyadenylated, messenger RNA, RNS, 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cervical vertebra, atlas, protein_coding_transcript, cervical vertebra 1, mRNA, ribose nucleic acid, Non Polyadenylated RNA, C1, ligand, Proteins, ribonucleic acids, Non-Polyadenylated, messenger RNA, RNS, proteins, Ribonucleic Acid, Non-Polyadenylated RNA, Protein Gene Products, Gene Proteins, polypeptide, template RNA, vertebra 1, yeast nucleic acid, Ribonukleinsaeure, Protein, Gene Products, INSDC_feature:mRNA, pentosenucleic acids, Ribonucleic acids, atlas (CI), cervical atlas, atlas vertebra, Non Polyadenylated, RNA Gene Products, C1 vertebra, atlas [C I]"],"pubmed_abstract_synonyms":["Ribonucleic, Amino Acid Motif, human being, Biocatalysts, Gene, Visible Light, biosynthesis, Homo sapiense, composed of, Homo spaiens, Homo sapien, Double Stranded RNA Binding Proteins, Supersecondary Protein Structure, Homo sapians, Gene Products, light, energy pathways, Non Polyadenylated, RNA Gene Products, RNA Binding Proteins, multicellular organismal biosynthetic process, Protein Motif, study, 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Experiment: KS_A4_3, file: folder summary. Published as part of Cell. 2012 Jun 8;149(6):1393-406  . From the Abstract: {{i}} RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed \"interactome capture,\" to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. {{/i}}","dates":{"submission":"2015-09-16"},"accession":"GPM11210041750","cross_references":{"pubmed":["22658674"],"Pride":["PXD002880"],"pride":[],"Pride Archive":["PXD002880"]}}