{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":25,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Guyonnet B, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Abelson_murine_leukemia_virus_uid14654,ectromelia_virus_uid14211,minute_virus_of_mice_uid14019,moloney_murine_leukemia_virus_uid15030,moloney_murine_sarcoma_virus_uid14721,mouse_mammary_tumor_virus_uid14435,mouse_parvovirus_1_uid14325,mouse_parvovirus_2_uid17125,mouse_parvovirus_3_uid17123,mouse_parvovirus_4a_uid33009,mouse_parvovirus_5a_uid33007,murid_herpesvirus_1_uid15181,murid_herpesvirus_2_uid14419,murid_herpesvirus_4_uid14458,murine_adenovirus_3_uid37713,murine_adenovirus_a_uid14519,murine_hepatitis_virus_a59_uid15350,murine_hepatitis_virus_jhm_uid15138,murine_norovirus_1_uid17577,murine_osteosarcoma_virus_uid14655,murine_pneumotropic_virus_uid14071,murine_polyomavirus_uid15489,murine_type_c_retrovirus_uid15204, Mouse"],"publication":["24797071"],"submitter_mail":["benoit.guyonnet@live.fr"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000032","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000031","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000030","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000040","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000029","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000039","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000038","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000037","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000036","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000035","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000034"],"submitter_affiliation":["Texas Tech University Health Sciences Center, Cell Biology and Biochemistry Department"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["The acrosomal matrix (AM) is an insoluble structure within the sperm acrosome that serves as a scaffold controlling the release of AM-associated proteins during the sperm acrosome reaction. The AM also interacts with the zona pellucida (ZP) that surrounds the oocyte, suggesting a remarkable stability that allows its survival despite being surrounded by proteolytic and hydrolytic enzymes released during the acrosome reaction. To date, the mechanism responsible for the stability of the AM is not known. Our studies demonstrate that amyloids are present within the sperm AM and contribute to the formation of an SDS- and formic-acid-resistant core. The AM core contained several known amyloidogenic proteins, as well as many proteins predicted to form amyloid, including several ZP binding proteins, suggesting a functional role for the amyloid core in sperm-ZP interactions. While stable at pH 3, at pH 7, the sperm AM rapidly destabilized. The pH-dependent dispersion of the AM correlated with a change in amyloid structure leading to a loss of mature forms and a gain of immature forms, suggesting that the reversal of amyloid is integral to AM dispersion."],"pubmed_title":["Functional amyloids in the mouse sperm acrosome."],"pubmed_authors":["Guyonnet Benoit B,Egge Nathan N,Cornwall Gail A GA,","Guyonnet Benoit B, Egge Nathan N, Cornwall Gail A GA"],"name_synonyms":["semen, Mus musculus, 3.4.22.-, sperm cell, acrosomal granule, Caspase-14 subunit p10, Laboratory, Swiss, Acrosomes., mice, Mus domesticus, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, \"mouse\" EXACT common_name [], MICE, spermatozoid, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, spermatozoon, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], seminal fluid, acrosome, male gamete, House, Mus, sperm, Mini-ICE, Mus musculus domesticus, spermatazoon, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], mature sperm cell, Mouse, SPERM, house mouse, Mice, Laboratory Mouse, ejaculate"],"description_synonyms":["sodium salt, vitelline envelope, ammonium formate, 13C-labeled, cadmium salt, nickel formate dihydrate, insensitive, conformation, lead formate, mol, Biocatalysts, Acrosome Reactions, yolk sac membrane, enclosed by, aluminum salt, cesium salt, nucleocapsid, zinc formate, Gene, lead salt, biosynthesis, magnesium formate, zinc salt, formic acid, SDS, potassium salt, ammonium tetraformate, cobalt(II) formate dihydrate, 14C-labeled, Gene Products, mature sperm cell, multicellular organismal biosynthetic process, insoluble, sperm cell, single-organism biosynthetic process, acrosomal granule, reference sample, enzymes, formation, thallium (+1) salt, cell, anabolism, rubidium salt, 4733401P19Rik, proteins, present in organism, copper, calcium formate, methanoic acid, synthesis, acrosome, lithium salt, sperm, Reaction, SPERM, cromium (+3) salt, zona pellucida - vitelline membrane, associated, relational structural quality, ammonium (2:1) salt, Acrosome, sodium formate, Controlled, nickel salt, ejaculate, data, Controlling, nickel formate, cobalt (+2) salt, Acrosomes, Proteins, zona radiata, vitelline membrane, AI836084, strontium formate, enzyme activity, oolemma, potassium formate, copper (+2) salt, strontium salt, Cell, spermatozoon, polypeptide, sodium (4:1:1) salt, male gamete, magnesium salt, cupric formate, Protein, zona pellucida, aluminum formate, nickel (+2) salt, semen, ammonium salt, lithium formate, Reactions, striated membrane, core., resistant, formate, oogonium, cobaltous formate, SWDS, Shwachman-Bodian-Diamond syndrome, cromium (+3), Shwachman syndrome, m. vitellina, CGI-97, spermatozoid, chromic formate, Protein Gene Products, seminal fluid, Gene Proteins, pellucid zone, Pancreatic insufficiency and bone marrow dysfunction, copper salt, ammonium (4:1) salt, calcium salt, spermatazoon, zona striata, lead (+2) salt"],"pubmed_title_synonyms":["semen, Mus musculus, 3.4.22.-, sperm cell, acrosomal granule, Caspase-14 subunit p10, Laboratory, Swiss, Acrosomes., mice, Mus domesticus, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, \"mouse\" EXACT common_name [], MICE, spermatozoid, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, spermatozoon, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], seminal fluid, acrosome, male gamete, House, Mus, sperm, Mini-ICE, Mus musculus domesticus, spermatazoon, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], mature sperm cell, Mouse, SPERM, house mouse, Mice, Laboratory Mouse, ejaculate"],"pubmed_abstract_synonyms":["vitelline envelope, Controlling, insensitive, conformation, Biocatalysts, Acrosomes, Acrosome Reactions, yolk sac membrane, enclosed by, Proteins, nucleocapsid, zona radiata, Amyloidogenic, vitelline membrane, AI836084, Gene, enzyme activity, oolemma, biosynthesis, predicted, spermatozoon, Concept, polypeptide, Amyloid Fibrils, SDS, survival, Role Concept, male gamete, Roles, Protein, PH 3, Gene Products, Role, core, Concepts, mature sperm cell, zona pellucida, multicellular organismal biosynthetic process, insoluble, semen, sperm cell, single-organism biosynthetic process, acrosomal granule, Amyloid Substance, Reactions, striated membrane, reference sample, death rate, enzymes, resistant, underdeveloped, formation, anabolism, ligand, Amyloid., oogonium, 4733401P19Rik, SWDS, Shwachman-Bodian-Diamond syndrome, proteins, present in organism, Shwachman syndrome, m. vitellina, CGI-97, Substance, Amyloid, spermatozoid, Protein Gene Products, synthesis, loss of, seminal fluid, Gene Proteins, pellucid zone, acrosome, Amyloid Proteins, Fibrils, sperm, time of survival, Pancreatic insufficiency and bone marrow dysfunction, Reaction, Role Concepts, spermatazoon, SPERM, zona pellucida - vitelline membrane, zona striata, associated, relational structural quality, Acrosome, Controlled, ejaculate"],"view_count":["25"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000037"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.0077112893275755705"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Functional Amyloids in the Mouse Sperm Acrosome.","description":"Data from ProteomeXchange, PXD ID: PXD000592. File: 130614_CornwallG_GB_GC3_HILIC.mzML. Published as part of Mol Cell Biol. 2014 May 5  . From the Abstract: {{i}} The acrosomal matrix (AM) is an insoluble structure within the sperm acrosome that serves as a scaffold controlling the release of AM associated proteins during the sperm acrosome reaction. The AM also interacts with the zona pellucida (ZP) that surrounds the oocyte suggesting a remarkable stability to survive despite being surrounded by proteolytic and hydrolytic enzymes released during the acrosome reaction. To date, the mechanism for the stability of the AM is not known. Our studies demonstrate that amyloids are present within the sperm AM and contribute to the formation of an SDS and formic acid resistant core. {{/i}}","dates":{"submission":"2014-05-07"},"accession":"GPM32310000037","cross_references":{"pubmed":["24797071"],"Pride":["PXD000592"],"pride":[],"Pride Archive":["PXD000592"]}}