GPMDB00196OtherWilhelm M, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humankuster@tum.de24870543Technische Universitaet Muenchen Chair of Proteomics and 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availableGPMDBProteomes are characterized by large protein-abundance differences, cell-type- and time-dependent expression patterns and post-translational modifications, all of which carry biological information that is not accessible by genomics or transcriptomics. Here we present a mass-spectrometry-based draft of the human proteome and a public, high-performance, in-memory database for real-time analysis of terabytes of big data, called ProteomicsDB. The information assembled from human tissues, cell lines and body fluids enabled estimation of the size of the protein-coding genome, and identified organ-specific proteins and a large number of translated lincRNAs (long intergenic non-coding RNAs). Analysis of messenger RNA and protein-expression profiles of human tissues revealed conserved control of protein abundance, and integration of drug-sensitivity data enabled the identification of proteins predicting resistance or sensitivity. The proteome profiles also hold considerable promise for analysing the composition and stoichiometry of protein complexes. ProteomicsDB thus enables navigation of proteomes, provides biological insight and fosters the development of proteomic technology.Mass-spectrometry-based draft of the human proteome.Wilhelm Mathias M,Schlegl Judith J,Hahne Hannes H,Moghaddas Gholami Amin A,Lieberenz Marcus M,Savitski Mikhail M MM,Ziegler Emanuel E,Butzmann Lars L,Gessulat Siegfried S,Marx Harald H,Mathieson Toby T,Lemeer Simone S,Schnatbaum Karsten K,Reimer Ulf U,Wenschuh Holger H,Mollenhauer Martin M,Slotta-Huspenina Julia J,Boese Joos-Hendrik JH,Bantscheff Marcus M,Gerstmair Anja A,Faerber Franz F,Kuster Bernhard B,Wilhelm Mathias M,Schlegl Judith J,Hahne Hannes H,Gholami Amin Moghaddas AM,Lieberenz Marcus M,Savitski Mikhail M MM,Ziegler Emanuel E,Butzmann Lars L,Gessulat Siegfried S,Marx Harald H,Mathieson Toby T,Lemeer Simone S,Schnatbaum Karsten K,Reimer Ulf U,Wenschuh Holger H,Mollenhauer Martin M,Slotta-Huspenina Julia J,Boese Joos-Hendrik JH,Bantscheff Marcus M,Gerstmair Anja A,Faerber Franz F,Kuster Bernhard B,Wilhelm Mathias M, Schlegl Judith J, Hahne Hannes H, Gholami Amin Moghaddas AM, Lieberenz Marcus M, Savitski Mikhail M MM, Ziegler Emanuel E, Butzmann Lars L, Gessulat Siegfried S, Marx Harald H, Mathieson Toby T, Lemeer Simone S, Schnatbaum Karsten K, Reimer Ulf U, Wenschuh Holger H, Mollenhauer Martin M, Slotta-Huspenina Julia J, Boese Joos-Hendrik JH, Bantscheff Marcus M, Gerstmair Anja A, Faerber Franz F, Kuster Bernhard BHomo sapients, human being, Spectrum, Spectrometry, FBN, Humo sapiens, MASS, Homo sapiense, man, Homo sapines, human, OCTD, ACMICD, Spectroscopy, Homo spaiens, Homo spiens, "human" EXACT genbank_common_name [], Homo sapien, Homo sapience, ECTOL1, Homo sapian, Homo sampiens, Homo sapians, SSKS, Proteomes., MFS1, Analysis, Homo sapeins, GPHYSD2, Home sapiens, WMS, WMS2, SGSdata.Homo sapients, human being, Spectrum, Spectrometry, FBN, Humo sapiens, MASS, Homo sapiense, man, Homo sapines, human, OCTD, ACMICD, Spectroscopy, Homo spaiens, Homo spiens, "human" EXACT genbank_common_name [], Homo sapien, Homo sapience, ECTOL1, Homo sapian, Homo sampiens, Homo sapians, SSKS, Proteomes., MFS1, Analysis, Homo sapeins, GPHYSD2, Home sapiens, WMS, WMS2, SGSbig, human being, single-organism developmental process, determination, Effects, number, A4, FBN, Gene, Homo sapiense, composed of, TYPE, Long Term, element, DAGA4, period, large, Homo spaiens, Homo sapien, ECTOL1, Homo sapians, resistance, Line, Gene Products, Analysis, MAM, SCG3, Effect, 2410008O07Rik, sensitivity, WMS, organ, Homo sapients, reference sample, Genomes, Identification, Longterm, cell, long, Spectrum, AA960360, Tissue, cell line cell, messenger RNA, Big-3, composition, proteins, present in organism, Long-Term, number of, man, cell line, OCTD, template RNA, drugs, Homo sapience, medicine, anatomical unit, Homo sampiens, great, body organ, has or lacks parts of type, Sensitivity, Long-Term Effect, GPHYSD2, Home sapiens, Long-Term Effects, SGS, Controlled, data, Controlling, drug, Arts, Proteins, Longterm Effect, Cell Lines, Fluids, cell_line, extra or missing physical or functional parts, compositionality, Cell, LGMD2C, ACMICD, Spectroscopy, mereological quality, polypeptide, development, Fluid, Homo sapian, Long Term Effects, chemical analysis, Protein, INSDC_feature:mRNA, MFS1, Homo sapeins, WMS2, Data Base, Lines, AA408785, Body Fluid, protein_coding_transcript, Industrial Arts, DMDA1, mRNA, content, expanded, Spectrometry, Specificity, Humo sapiens, Identifications (Psychology), MASS, whole genome, Homo sapines, human, Body, Longterm Effects, Protein Gene Products, Big, Gene Proteins, Homo spiens, DMDA, "human" EXACT genbank_common_name [], enlarged, Specificity and Sensitivity, SCARMD2, SSKS, cardinality, structure, assay, Industrial., Proteomes, time1906http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310000771dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0tls_masters~0mesh~0coding~0biotools~1sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0058605798889574340.01.0falseMass-spectrometry-based draft of the human proteomeData from ProteomeXchange, PXD ID: PXD000865. File: 00645_A01_P004893_B0A_A00_R1.mzml. Published as part of Nature 509, 582-587, 29 May 2014.2014-05-30GPM3231000077124870543PXD000865PXD000865