GPMDB00275OtherAlqahtani A, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humand.a.matthews@bristol.ac.uk25096814http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002894http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002884http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002895http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002896http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002885http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002897http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002886http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002898http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002887http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002899http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002888http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002889http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002900http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002901http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002890http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002891http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002892http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002893http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002902http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002903http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310002905School of Cellular and Molecular Medicine, University of Bristol, et al.Not availableGPMDBWe used SILAC (stable isotope labelling of amino acids in cell culture) and high-throughput quantitative MS mass spectrometry to analyse the protein composition of highly purified WT wild type adenoviruses, mutant adenoviruses lacking an internal protein component (protein V) and recombinant adenoviruses of the type commonly used in gene therapy, including one virus that had been used in a clinical trial. We found that the viral protein abundance and composition were consistent across all types of virus examined except for the virus lacking protein V, which also had reduced amounts of another viral core protein, protein VII. In all the samples analysed we found no evidence of consistent packaging or contamination with cellular proteins. We believe this technique is a powerful method to analyse the protein composition of this important gene therapy vector and genetically engineered or synthetic virus-like particles. The raw data have been deposited at proteomexchange, identifer PXD001120.Analysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics.Alqahtani Ali A,Heesom Kate K,Bramson Jonathan L JL,Curiel David D,Ugai Hideyo H,Matthews David A DA,Alqahtani Ali A, Heesom Kate K, Bramson Jonathan L JL, Curiel David D, Ugai Hideyo H, Matthews David A DADAGA4, count in organism, presence or absence in organism., DMDA, count, DMDA1, determination, chemical analysis, SCARMD2, number, A4, assay, quantitative, MAM, SCG3, presence, TYPE, LGMD2CViridae, Product, Aminosaeure, Apo-2L, nucleocapsid, Aminocarbonsaeure, number, A4, Gene, Spectrum Analyses, TNFSF10, composed of, presence, TYPE, DAGA4, TRAIL, reduced, subnumerary, Mass, Virus, Gene Products, Analysis, Animal, tiny, MAM, SCG3, present in fewer numbers in organism, Mass Spectroscopy, Gametic Genetic Therapy, Mass Spectrum Analysis, Somatic, virus protein, Animal Virus, amino acids, Genetic, Containers, Viruses, Analyses, Somatic Genetic Therapies, Gametic Genetic Therapies, DNA Therapy, hypoplasia, composition, labeling, proteins, decreased number, decreased, Vira, Therapies, Product Container, CD253, small, Product Packagings, Therapy, Genetic Therapy, protein TRAIL, Gene., data, apo-2 ligand, Aminokarbonsaeure, Proteins, Container, compositionality, Spectrum Analysis, Somatic Genetic Therapy, LGMD2C, Spectroscopy, polypeptide, Somatic Genetic, viruses, count in organism, Genetic Therapies, Gene Therapy, count, Gametic, Protein, core, Zoophaginae, Packaging, viral-, TL2, Mass Spectrum Analyses, Mass Spectrum, apo-2L, Gametic Genetic, DMDA1, underdeveloped, Somatic Gene Therapy, content, Spectrometry, Somatic Gene, Amino, TNF-related apoptosis-inducing ligand, Protein Gene Products, Gene Proteins, APO2L, Packagings, DMDA, Product Containers, SCARMD2, structure, Animal Viruses, Acids, quantitative, DNA, presence or absence in organismDAGA4, count in organism, presence or absence in organism., DMDA, count, DMDA1, determination, chemical analysis, SCARMD2, number, A4, assay, quantitative, MAM, SCG3, presence, TYPE, LGMD2CViridae, Bru, artificial sequence, Product, Procedures, Raw, Aminosaeure, nucleocapsid, Aminocarbonsaeure, number, A4, Gene, Spectrum Analyses, composed of, presence, TYPE, DAGA4, method, reduced, subnumerary, Method, method used in an experiment, Mass, Virus, Gene Products, Studies, Del(8)44H, Analysis, Animal, tiny, synthetic genetic interaction (sensu inequality), MAM, SCG3, present in fewer numbers in organism, Mass Spectroscopy, Gametic Genetic Therapy, Mass Spectrum Analysis, Svc, Somatic, virus protein, Animal Virus, amino acids, Genetic, Containers, Viruses, Analyses, Somatic Genetic Therapies, Gametic Genetic Therapies, DNA Therapy, Intervention Study, hypoplasia, synthetic genetic interaction defined by inequality, composition, labeling, proteins, decreased number, Study, decreased, Methodological Studies, Vira, data., Therapies, Product Container, small, Product Packagings, Therapy, Genetic Therapy, Aminokarbonsaeure, Proteins, Container, artificial gene, compositionality, Procedure, synthetic DNA, Spectrum Analysis, Somatic Genetic Therapy, LGMD2C, Spectroscopy, polypeptide, Somatic Genetic, viruses, count in organism, Genetic Therapies, Gene Therapy, count, Gametic, Protein, core, synthetic, Zoophaginae, Packaging, viral-, Mass Spectrum Analyses, Mass Spectrum, Gametic Genetic, Col4a-1, DMDA1, underdeveloped, Somatic Gene Therapy, content, Spectrometry, Somatic Gene, Methodological, Amino, Methodological Study, synthetic constructs, Protein Gene Products, plan specification, Gene Proteins, Packagings, SYNTHETIC CONSTRUCT sequences, DMDA, Product Containers, SCARMD2, structure, artificial, Animal Viruses, Acids, quantitative, DNA, presence or absence in 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archive~1varsite~00Resource Reanalysis0.00.00.0083281924737816160.01.0falseAnalysis of purified wild type and mutant adenovirus particles by SILAC based quantitative proteomics.Data from ProteomeXchange, PXD ID: PXD001120. File: 10_2.mzml. Published as part of J Gen Virol. 2014 Aug 5 . From the Abstract: {{i}} We used stable isotope labelling of amino acids in cell culture and high throughput quantitative mass spectrometry to analyse the protein composition of highly purified wild type adenoviruses, mutant adenoviruses lacking an internal protein component (protein V) and recombinant adenoviruses of the type commonly used in gene therapy including one virus which had been used in a clinical trail. We found that the viral protein abundance and composition was consistent across all types of virus examined except for the virus lacking protein V which also had reduced amounts of another viral core protein, protein VII. In all the samples analysed we found no evidence of consistent packaging or contamination with cellular proteins ... {{/i}}2014-08-13GPM3231000288525096814PXD001120PXD001120