GPMDB00197OtherCarvalho AS, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanrunem2009@gmail.com25128556http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003279http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003283http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003293http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003282http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003285http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003284http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003287http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003276http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003286http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003278http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003289http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003277http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003288http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003290http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003292http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003291http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003280INSA, Portugal, et al.Not availableGPMDBWe investigated the molecular effects of glucosamine supplements, a popular and safe alternative to nonsteroidal anti-inflammatory drugs, for decreasing pain, inflammation, and maintaining healthy joints. Numerous studies have reported an array of molecular effects after glucosamine treatment. We questioned whether the differences in the effects observed in previous studies were associated with the focus on a specific subproteome or with the use of specific cell lines or tissues. To address this question, global mass spectrometry- and transcription array-based glucosamine drug profiling was performed on malignant cell lines from different stages of lymphocyte development. We combined global label-free MS-based protein quantitation with an open search for modifications to obtain the best possible proteome coverage. Our data were largely consistent with previous studies in a variety of cellular models. We mainly observed glucosamine induced O-GlcNAcylation/O-GalNAcylation (O-HexNAcylation); however, we also observed global and local changes in acetylation, methylation, and phosphorylation. For example, our data provides two additional examples of "yin-yang" between phosphorylation and O-HexNAcylation. Furthermore, we mapped novel O-HexNAc sites on GLU2B and calnexin. GLU2B and calnexin are known to be located in the endoplasmic reticulum (ER) and involved in protein folding and quality control. The O-HexNAc sites were regulated by glucosamine treatment and correlated with the up-regulation of the ER stress marker GRP78. The occupancy of O-HexNAc on GLU2B and calnexin sites differed between the cytosolic and nuclear fractions with a higher occupancy in the cytosolic fraction. Based on our data we propose the hypothesis that O-HexNAc either inactivates calnexin and/or targets it to the cytosolic fraction. Further, we hypothesize that O-HexNAcylation induced by glucosamine treatment enhances protein trafficking.Global mass spectrometry and transcriptomics array based drug profiling provides novel insight into glucosamine induced endoplasmic reticulum stress.Carvalho Ana Sofia AS,Ribeiro Helena H,Voabil Paula P,Penque Deborah D,Jensen Ole N ON,Molina Henrik H,Matthiesen Rune R,Carvalho Ana Sofia AS, Ribeiro Helena H, Voabil Paula P, Penque Deborah D, Jensen Ole N ON, Molina Henrik H, Matthiesen Rune RMass Spectrum Analysis, Mass Spectrum, Glucosamine, D-Glucose, Glucosamine Sulfate, 2-amino-2-deoxyglucose, Dona S, glucosamine, Analyses, drug, Spectrometry, Glucosamin, Hespercorbin, 2-Amino-2-Deoxyglucose, Spectrum Analyses, C6H13NO5, Spectrum Analysis, Xicil, 2-amino-2-deoxy-, Glukosamin, 2 Amino 2 Deoxyglucose, Spectroscopy, drugs, medicine, Mass, GlcN, microarray, 2-Amino-2-deoxy-glucose, Analysis, ER stress., Sulfate, Mass Spectrum Analyses, Mass Spectroscopy, DonaBurning Pain, joint, data, transcription, cellular transcription, joints, use, 2-amino-2-deoxyglucose, Dona S, Crushing Pains, Inflammations, DNA-dependent transcription, mol, drug, Physical, Cell Lines, Migratory Pains, Splitting Pain, Physical Sufferings, cell_line, Radiating Pain, Spectrum Analyses, Sufferings, Xicil, Spectrum Analysis, Cell, 2-amino-2-deoxy-, Glukosamin, Ache, Spectroscopy, Crushing Pain, Experiment, Pains, Joint, Line, Mass, GlcN, 2-Amino-2-deoxy-glucose, Migratory, transcription regulator activity, lymphocyte cell differentiation, Analysis, Suffering, Mass Spectrum Analyses, Mass Spectroscopy, Dona, Lines, Mass Spectrum Analysis, treatment, Glucosamine, D-Glucose, Mass Spectrum, NSAID, Crushing, lymphocytic blood cell differentiation, lymphocyte development., Glucosamine Sulfate, articulation, FOCUS, glucosamine, inflammatory response, Analyses, cell, non-steroidal anti-inflammatory agent, Tissue, Spectrometry, Glucosamin, Pain, cell line cell, Hespercorbin, inflammation, NSAIDs, Aches, 2-Amino-2-Deoxyglucose, Splitting Pains, C6H13NO5, Migratory Pain, Radiating, cell line, 2 Amino 2 Deoxyglucose, DNA-dependent, drugs, Radiating Pains, non-steroidal anti-inflammatory drugs, Inflammation, medicine, disease management, Burning, articular joint, Splitting, microarray, Burning Pains, Physical Suffering, associated, SulfateMass Spectrum Analysis, Mass Spectrum, Glucosamine, D-Glucose, Glucosamine Sulfate, 2-amino-2-deoxyglucose, Dona S, glucosamine, Reticulum Stress, Analyses, Endoplasmic Reticulum, drug, Spectrometry, Glucosamin, Hespercorbin, 2-Amino-2-Deoxyglucose, Spectrum Analyses, C6H13NO5, Spectrum Analysis, Xicil, 2-amino-2-deoxy-, Glukosamin, 2 Amino 2 Deoxyglucose, Stresses, Spectroscopy, drugs, Endoplasmic, ER stress, medicine, Endoplasmic Reticulum Stresses, Stress, Mass, GlcN, Reticulum Stresses, microarray, 2-Amino-2-deoxy-glucose, Analysis, Sulfate, Mass Spectrum Analyses, Endoplasmic., Mass Spectroscopy, DonaBurning Pain, AU019543, transcription, joints, 2-amino-2-deoxyglucose, Dona S, Inflammations, Globular Protein Folding, Integral Membrane Protein IP90, mBiP, HEL-S-89n, Physical, Foldings, Migratory Pains, Splitting Pain, Radiating Pain, Spectrum Analyses, beta-tubulin folding, baffled, Sufferings, Xicil, Glukosamin, phosphorylation, pp90, D2Wsu141e, AL022860, Joint, chaperonin ATPase activity, Line, Mass, GlcN, ARB, non-chaperonin molecular chaperone ATPase activity, glycoprotein-specific chaperone activity, Heat shock 70 kDa protein 5, Migratory, transcription regulator activity, lymphocyte cell differentiation, Analysis, Steroidogenesis-activator polypeptide, Hsce70, GRP-78, Globular, Suffering, Mass Spectroscopy, Mass Spectrum Analysis, SEZ-7, treatment, Glucosamine, Glucosamine Sulfate, FOCUS, inflammatory response, anti-inflammatory drugs, Analyses, Receptor Up-Regulation, VMD2, Up Regulation, Tissue, Glucosamin, Acetylations, cell line cell, Hespercorbin, inflammation, Aches, proteins, 2-Amino-2-Deoxyglucose, BMD, Phosphoprotein, free, cell line, 2 Amino 2 Deoxyglucose, Folding, Growth|differentiation factor 10, chaperonin-mediated tubulin folding, DNA-dependent, proteins., drugs, GDF-10, Quality Controls, GRP78, Inflammation, medicine, Bone-inducing protein, MIF2, Protein Folding, anti-inflammatory agent, articular joint, disease management, Splitting, Burning Pains, associated, Grp78, Methylations, RP50, joint, IP90 Protein, cellular transcription, data, use, Crushing Pains, DNA-dependent transcription, Ergastoplasm, drug, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, acetylation, Cell Lines, Phosphorylations, Up-Regulation (Physiology), Immunoglobulin heavy chain-binding protein, D2Wsu17e, Physical Sufferings, cell_line, Spectrum Analysis, 2-amino-2-deoxy-, Cell, Ache, Spectroscopy, Reticulum, polypeptide, chaperone activity, Crushing Pain, Globular Protein, Pains, co-chaperonin activity, ER stress, ER, Protein, 2-Amino-2-deoxy-glucose, alpha-tubulin folding, methylation, Protein Foldings, TU15B, protein complex assembly, Mass Spectrum Analyses, Dona, Lines, D-Glucose, Mass Spectrum, Crushing, lymphocytic blood cell differentiation, articulation, glucosamine, Sez7, BiP, lymphocyte development, Upregulation, Spectrometry, Control, Pain, Quality, BMP-3B, Splitting Pains, Controls, C6H13NO5, Yin Yang, Migratory Pain, Radiating, co-chaperone activity, Endoplasmic, Globular Protein Foldings, Radiating Pains, Bip, BIP, Burning, multichaperone pathway, microarray, Physical Suffering, Sulfate, BEST, Proteomes, Phosphoprotein pp90, hypothesis1907http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310003280dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0pride~1human_diseases~0embl~0geo_datasets~1treefam~0project~1uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0058605798889574340.01.0falseGlobal mass spectrometry and transcriptomics array based drug profiling provides novel insight into glucosamine induced ER stress.Data from ProteomeXchange, PXD ID: PXD000380. Experiment: Treatment, file: MS12236QE_rune_henrik_Treatment_c.mzml. Published as part of Mol Cell Proteomics. 2014 Aug 15 . From the Abstract: {{i}} We investigated the molecular effects of glucosamine supplements, a popular and safe alternative to non-steroidal anti-inflammatory drugs, for decreasing pain, inflammation and maintaining healthy joints. Numerous studies have reported an array of molecular effects after glucosamine treatment. We questioned whether the differences in the effects observed in previous studies were associated with the focus on a specific subproteome or with the use of specific cell lines or tissues. To address this question, global mass spectrometry- and transcription array-based glucosamine drug profiling was performed on malignant cell lines from different stages of lymphocyte development ... {{/i}}2014-08-21GPM3231000328025128556PXD000380PXD000380