GPMDB00395OtherSong Z, et al.InstrumentNot AvailableNot availableSynechococcus_phage_p60_uid14628,synechococcus_phage_syn5_uid19763,synechococcus_phage_syn9_uid17541,synechococcus_phage_s_pm2_uid15223,synechococcus_phage_s_rsm4_uid39923, Synechocystis_sp_pcc_6803wwzhang8@tju.edu.cn25239498Tianjin University, China, et al.http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004127http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004116http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004115http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004126http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004129http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004118http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004128http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004119http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004130http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004121http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004132http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004120http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004131http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004112http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004123http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004122http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004114http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004125http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004113http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004124Not availableGPMDBTo improve ethanol production directly from CO2 in photosynthetic cyanobacterial systems, one key issue that needs to be addressed is the low ethanol tolerance of cyanobacterial cells. Our previous proteomic and transcriptomic analyses found that several regulatory proteins were up-regulated by exogenous ethanol in Synechocystis sp. PCC6803. In this study, through tolerance analysis of the gene disruption mutants of the up-regulated regulatory genes, we uncovered that one transcriptional regulator, Sll0794, was related directly to ethanol tolerance in Synechocystis. Using a quantitative iTRAQ-LC-MS/MS proteomics approach coupled with quantitative real-time reverse transcription-PCR (RT-qPCR), we further determined the possible regulatory network of Sll0794. The proteomic analysis showed that in the Δsll0794 mutant grown under ethanol stress a total of 54 and 87 unique proteins were down- and up-regulated, respectively. In addition, electrophoretic mobility shift assays demonstrated that the Sll0794 transcriptional regulator was able to bind directly to the upstream regions of sll1514, slr1512, and slr1838, which encode a 16.6 kDa small heat shock protein, a putative sodium-dependent bicarbonate transporter and a carbon dioxide concentrating mechanism protein CcmK, respectively. The study provided a proteomic description of the putative ethanol-tolerance network regulated by the sll0794 gene, and revealed new insights on the ethanol-tolerance regulatory mechanism in Synechocystis. As the first regulatory protein discovered related to ethanol tolerance, the gene may serve as a valuable target for transcription machinery engineering to further improve ethanol tolerance in Synechocystis. All MS data have been deposited in the ProteomeXchange with identifier PXD001266 (http://proteomecentral.proteomexchange.org/dataset/PXD001266).A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803.Song Zhongdi Z,Chen Lei L,Wang Jiangxin J,Lu Yinhua Y,Jiang Weihong W,Zhang Weiwen W,Song Zhongdi Z, Chen Lei L, Wang Jiangxin J, Lu Yinhua Y, Jiang Weihong W, Zhang Weiwen WPyro, Thiofaco M-50, Grain Alcohol, Synechocystis 6803, Ethanol Absolute, Denatured Alcohol, etanol, USAF EK-1597, Aphanocapsa sp. 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ATCC 27184, presence or absence in organism3905http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004118dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0120296113510178910.01.0falseA transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803.Data from ProteomeXchange, PXD ID: PXD001266. File: zao-07.mgf. Published as part of Mol Cell Proteomics. 2014 Sep 19 . From the Abstract: {{i}} To improve ethanol production directly from CO2 in photosynthetic cyanobacterial systems, one key issue that needs to be addressed is the low ethanol tolerance of cyanobacterial cells. Our previous proteomic and transcriptomic analyses found that several regulatory proteins were up regulated by exogenous ethanol in Synechocystis sp. PCC6803. In this study, through tolerance analysis of the gene disruption mutants of the up-regulated regulatory genes, we uncovered that one transcriptional regulator, Sll0794, was related directly to ethanol tolerance in Synechocystis ... {{/i}}2014-09-26GPM3231000411825239498PXD001266PXD001266