GPMDB00213OtherAlli Shaik A, et al.InstrumentNot AvailableNot availableFish_ejayanthag@imcb.a-star.edu.sg25230361http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004248http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004468http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004338http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004403http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004416http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004429http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004273http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004184http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004481http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004286http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004351http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004494http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004442http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004145http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004299http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004364http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004455http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004312http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004158http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004235http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004377http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004390http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004171Institute of Molecular and Cell Biology, SingaporeNot availableGPMDBZebrafish is a popular system for studying vertebrate development and disease that shows high genetic conservation with humans. Molecular level studies at different stages of development are essential for understanding the processes deployed during ontogeny. Here, we performed comparative analysis of the whole proteome and transcriptome of the early stage (24 h post-fertilization) zebrafish embryo. We identified 8363 proteins with their approximate cellular abundances (the largest number of zebrafish embryo proteins quantified thus far), through a combination of thorough deyolking and extensive fractionation procedures, before resolving the peptides by mass spectrometry. We performed deep sequencing of the transcripts and found that the expressed proteome and transcriptome displayed a moderate correlation for the majority of cellular processes. Integrative functional mapping of the quantified genes demonstrated that embryonic developmental systems differentially exploit transcriptional and post-transcriptional regulatory mechanisms to modulate protein abundance. Using network mapping of the low-abundance proteins, we identified various signal transduction pathways important in embryonic development and also revealed genes that may be regulated at the post-transcriptional level. Our data set represents a deep coverage of the functional proteome and transcriptome of the developing zebrafish, and our findings unveil molecular regulatory mechanisms that underlie embryonic development.Functional mapping of the zebrafish early embryo proteome and transcriptome.Alli Shaik Asfa A,Wee Sheena S,Li Rachel Hai Xia RH,Li Zhen Z,Carney Tom J TJ,Mathavan Sinnakaruppan S,Gunaratne Jayantha J,Alli Shaik Asfa A, Wee Sheena S, Li Rachel Hai Xia RH, Li Zhen Z, Carney Tom J TJ, Mathavan Sinnakaruppan S, Gunaratne Jayantha Jzebra fish, Danio rerio (Hamilton, leopard danio, advanced, Zebra Fish, Transcriptome, embryo, plant germ, Transcriptome Profile, Expression Profiles, Gene Expression Profile, Gene, Profiles, Cyprinus rerio Hamilton, developing organism, embryo stage, whole transcriptome, Gene Expression, zebra danio, Transcriptome Profiles., Expression Signatures, Danio rerio frankei, 1822), Gene Expression Signatures, 1822, precocious, Brachydanio rerio, Embryologic, Gene 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developmental tissue, Gene Expression Profiles, syngamy, Fertilizations, Modern Man, D. rerio, Eph2, assay, Signature, zebrafish, Proteomes, Bglap-rs1, NEP2, humans, Gpi1szebra fish, Danio rerio (Hamilton, leopard danio, advanced, Zebra Fish, Transcriptome, embryo, plant germ, Transcriptome Profile, Expression Profiles, Gene Expression Profile, Gene, Profiles, Cyprinus rerio Hamilton, developing organism, embryo stage, whole transcriptome, Gene Expression, zebra danio, Transcriptome Profiles., Expression Signatures, Danio rerio frankei, 1822), Gene Expression Signatures, 1822, precocious, Brachydanio rerio, Embryologic, Gene Expression Signature, Brachidanio rerio, Cyprinus rerio, Expression Profile, D. rerios, Zebra, Danio rerio, Danio frankei, Zebrafishes, Profile, Fishes, embryo early (growth) stage, Entire embryo, danio, Zebra danios, Signatures, early, Zebra danio, embryonic organism, developmental tissue, Expression Signature, Gene Expression Profiles, B. rerio, Zebra Fishes, D. 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Signature2103http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004416dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0varsite~00.00.00.0064774830351634790.001.0falseFunctional Mapping of the Zebrafish Early Embryo Proteome and TranscriptomeData from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00444 PASS00444]]. Replicate: 2, experiment: G_120105_01_zebrafish_band3, file: folder summary. Published as part of J Proteome Res. 2014 Sep 17 . From the Abstract: {{i}} Zebrafish is a popular system for studying vertebrate development and disease, and shows high genetic conservation with humans. Molecular level studies at different stages of development are essential for understanding the mechanisms deployed during ontogeny. Here, we performed comparative analysis of whole proteome and transcriptome at early-stage (24 hours post fertilization) zebrafish embryo ... {{/i}}2014-10-10GPM3231000441625230361