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To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each time point. After 3 h of flooding exposure, the fresh weight of soybeans increased, whereas the ATP content of soybean root tips decreased. Using a gel-free proteomic technique, a total of 114 phosphoproteins were identified in the root tip samples, and 34 of the phosphoproteins were significantly changed with respect to phosphorylation status after 3 h of flooding stress. Among these phosphoproteins, eukaryotic translation initiation factors were dephosphorylated, whereas several protein synthesis-related proteins were phosphorylated. The mRNA expression levels of sucrose phosphate synthase 1F and eukaryotic translation initiation factor 4 G were down-regulated, whereas UDP-glucose 6-dehydrogenase mRNA expression was up-regulated during growth but down-regulated under flooding stress. Furthermore, bioinformatic protein interaction analysis of flooding-responsive proteins based on temporal phosphorylation patterns indicated that eukaryotic translation initiation factor 4 G was located in the center of the network during flooding. Soybean eukaryotic translation initiation factor 4 G has homology to programmed cell death 4 protein and is implicated in ethylene signaling. The weight of soybeans was increased with treatment by an ethylene-releasing agent under flooding condition, but it was decreased when plants were exposed to an ethylene receptor antagonist. These results suggest that the ethylene signaling pathway plays an important role, via the protein phosphorylation, in mechanisms of plant tolerance to the initial stages of flooding stress in soybean root tips."],"pubmed_title":["Phosphoproteomics reveals the effect of ethylene in soybean root under flooding stress."],"pubmed_authors":["Yin Xiaojian X,Sakata Katsumi K,Komatsu Setsuko S,","Yin Xiaojian X, Sakata Katsumi K, Komatsu Setsuko S"],"name_synonyms":["radices (exact, CH2=CH2, ra&#237z (Spanish, ethylene, plural), ethene, soybeans, Aethen, Aethylen, aerial root (narrow), InChI=1/C2H4/c1-2/h1-2H2, root, GRO:0005338, climbing root (narrow)., soybean, all, cv. Wye, C2H4, radix (exact), C=C, exact), Glycine max, 根 (Japanese, H2C=CH2, InChIKey=VGGSQFUCUMXWEO-UHFFFAOYAE"],"description_synonyms":["OCT, IGF-I, data, PlexA2, advanced, steel factor, Root Tips, PLXN2, Slf, Root Tip, hematopoietic growth factor KL, developmental stage, land plants, Proteins, Somatomedin-C, Meristems, Phosphorylations, somatomedin, Gene, FPH2, compositionality, composed of, AA589422, supernumerary, sKITLG, Soy Bean, polypeptide, Root, AW457381, exact), weight, reduced, subnumerary, mechano growth factor, Protein, precocious, Gene Products, Igf-1, present in fewer numbers in organism, increased, SHEP7, mast cell growth factor, Soybean, increased number, content, Stem cell factor, soybeans, Bean, Soy Beans, somatomedin-C, Plant, SF, Kitl, 2810428A13Rik, composition, plants, proteins, STAT5, decreased number, Mast cell growth factor, free, early, soybean, Protein Gene Products, cv. Wye, Gene Proteins, present in greater numbers in organism, stem cell factor, 根端 (Japanese, higher plants, KL-1, decreased, IGF1, MGF, Glycine max, c-Kit ligand, structure, Mechano growth factor, KITLG, punta de la ra&#237z (Spanish, Beans, stage, Soluble KIT ligand, phosphorylation., SCF, responsive, Sl, Plxn2, time point, Tip, Proteomes, mKIAA0463, Soy, accessory, Tips"],"pubmed_title_synonyms":["radices (exact, CH2=CH2, ra&#237z (Spanish, ethylene, plural), ethene, soybeans, Aethen, Aethylen, aerial root (narrow), InChI=1/C2H4/c1-2/h1-2H2, root, GRO:0005338, climbing root (narrow)., soybean, all, cv. Wye, C2H4, radix (exact), C=C, exact), Glycine max, 根 (Japanese, H2C=CH2, InChIKey=VGGSQFUCUMXWEO-UHFFFAOYAE"],"pubmed_abstract_synonyms":["Oxidases, protein translation, gluco-hexose, Dehydrogenases, advanced, biological signaling, CH2=CH2, C6H12O6, determination, Glucose, Dehydrogenase, caspase-independent cell death, Glukose, Pflanze, developmental stage, Gene, Monohydrate, (DL)-Isomer, composed of, supernumerary, phosphorylation, temporal, viridiplantae, protein amino acid phosphorylation, Root, Initiation Factor, exact), C=C, reduced, Roles, subnumerary, Dextrose, Gene Products, regulated cell death, ethylene mediated signaling pathway, Concepts, RCD, non-apoptotic programmed cell death, ethylene signaling pathway, present in fewer numbers in organism, plantae, Reductases, treatment, increased, Soybean, soybeans, ethene, Root Tip., Soy Beans, Aethylen, messenger RNA, composition, plants, proteins, protein synthesis initiation, decreased number, free, nonapoptotic programmed cell death, soybean, biopolymerisation, 根端 (Japanese, template RNA, C2H4, extruding from, decreased, ethylene mediated signalling pathway, Initiation Factors, signaling process, Role Concepts, disease management, punta de la ra&#237z (Spanish, Beans, stage, time point, Tip, Anhydrous, Soy, incidence, single organism signaling, Tips, protein anabolism, protein biosynthetic process, Root Tips, biopolymerization, (beta-D)-Isomer, Root Tip, ethylene, exits through, land plants, Proteins, Meristems, Phosphorylations, InChI=1/C2H4/c1-2/h1-2H2, compositionality, Peptide, results, Concept, Soy Bean, polypeptide, Role Concept, weight, Protein, chemical analysis, ethylene signal transduction, precocious, protein formation, PCD, Role, INSDC_feature:mRNA, protein biosynthesis, sucrose phosphate synthase, (alpha-D)-Isomer, Reductase, L Glucose, D-Glucose, Factors, Peptide Initiation, Oxidase, protein_coding_transcript, translation initiation, growth pattern, mRNA, non-developmental growth, increased number, content, Bean, Aethen, Plant, exposed, early, Anhydrous Dextrose, signalling, ethene mediated signalling pathway, ethene mediated signaling pathway, Protein Gene Products, cv. Wye, Gene Proteins, present in greater numbers in organism, higher plants, L-Glucose, signalling process, protein synthesis, D Glucose, Glycine max, structure, (L)-Isomer, caspase-independent apoptosis, assay, H2C=CH2, responsive, Glucose Monohydrate, accessory, Glc, InChIKey=VGGSQFUCUMXWEO-UHFFFAOYAE"],"view_count":["68"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310004941"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.020974706971005553"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Phosphoproteomics Reveals the Effect of Ethylene in Soybean Root under Flooding Stress.","description":"Data from ProteomeXchange, PXD ID: PXD000898. File: 51 0 4.mgf. Published as part of J Proteome Res. 2014 Oct 24  . From the Abstract: {{i}} To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each time point. After 3 h of flooding exposure, the fresh weight of soybeans increased, whereas the ATP content of soybean root tips decreased. Using a gel-free proteomic technique, a total of 114 phosphoproteins were identified in the root tip samples, and 34 of the phosphoproteins were significantly changed with respect to phosphorylation status after 3 h of flooding stress ... {{/i}}","dates":{"submission":"2014-11-13"},"accession":"GPM32310004941","cross_references":{"pubmed":["25316100"],"Pride":["PXD000898"],"pride":[],"Pride Archive":["PXD000898"]}}