GPMDB0004OtherCubenas-Potts C, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_femaletharan.srikumar@gmail.com25367092Department of Biochemistry & Molecular Biology, Johns Hopkins Bloomberg School of Public Healthhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005150http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005151http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005152http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005153http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005147http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005148http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005146Not availableGPMDBSumoylation is essential for progression through mitosis, but the specific protein targets and functions remain poorly understood. In this study, we used chromosome spreads to more precisely define the localization of SUMO-2/3 (small ubiquitin-related modifier) to the inner centromere and protein scaffold of mitotic chromosomes. We also developed methods to immunopurify proteins modified by endogenous, untagged SUMO-2/3 from mitotic chromosomes. Using these methods, we identified 149 chromosome-associated SUMO-2/3 substrates by nLC-ESI-MS/MS. Approximately one-third of the identified proteins have reported functions in mitosis. Consistent with SUMO-2/3 immunolocalization, we identified known centromere- and kinetochore-associated proteins, as well as chromosome scaffold associated proteins. Notably, >30 proteins involved in chromatin modification or remodeling were identified. Our results provide insights into the roles of sumoylation as a regulator of chromatin structure and other diverse processes in mitosis. Furthermore, our purification and fractionation methodologies represent an important compliment to existing approaches to identify sumoylated proteins using exogenously expressed and tagged SUMOs.Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes.Cubeñas-Potts Caelin C,Srikumar Tharan T,Lee Christine C,Osula Omoruyi O,Subramonian Divya D,Zhang Xiang-Dong XD,Cotter Robert J RJ,Raught Brian B,Matunis Michael J MJ,Cubeñas-Potts Caelin C, Srikumar Tharan T, Lee Christine C, Osula Omoruyi O, Subramonian Divya D, Zhang Xiang-Dong XD, Cotter Robert J RJ, Raught Brian B, Matunis Michael J MJProtein Gene Products, Gene Proteins, polypeptide, Ubiquitin-like protein SMT3A, Identification, Smt3A, Smt3B, Smt3b, Protein, Gene Products, Proteins, Chromosome., Identifications (Psychology), Smt3h2, Gene, proteins, Ubiquitin-like protein SMT3B, associated, SMT3 homolog 2, Sentrin-2, SMT3 homolog 1, HSMT3, SUMO-2, SUMO-3establishment and maintenance of substance location, INSDC_feature:centromere, data, Procedures, Smt3b, AUTSX5, Proteins, Phases, DXS648, Smt3h2, Gene, Procedure, CCN3, Sentrin-2, QM, HSMT3, polypeptide, prophase chromosome, single organism localization, Mitotic M, IGFBP-9, Method, mitosis, Protein, establishment and maintenance of position, Studies, Gene Products, Mitotic M Phase, NOV, establishment and maintenance of localization, establishment and maintenance of substrate location, techniques, SMT3 homolog 2, M Phase, PlexA1, SMT3 homolog 1, centromere complex, pericentric region, study, Phase, Plxn1, M Phases, Centromeres, Mitoses, interphase chromosome, Chromosome., chromatid, proteins, single-organism localization, Ubiquitin-like protein SMT3B, Methodological, procedures, IGFBP9, Methodological Study, mKIAA4053, SUMO-2, SUMO-3, IBP-9, Protein Gene Products, Study, centromere, Gene Proteins, centric region, localisation, chromosome, Ubiquitin-like protein SMT3A, Methodological Studies, Chromosome, L10, C130088N23Rik, Smt3A, Smt3B, Mitotic M Phases, 2600013D04Rik, Mitotic, DXS648E, NOVh, establishment and maintenance of cellular component location, methodologyProtein Gene Products, Gene Proteins, polypeptide, Ubiquitin-like protein SMT3A, Identification, Smt3A, Smt3B, Smt3b, Protein, Gene Products, Proteins, Chromosome., Identifications (Psychology), Smt3h2, Gene, proteins, Ubiquitin-like protein SMT3B, associated, SMT3 homolog 2, Sentrin-2, SMT3 homolog 1, HSMT3, SUMO-2, SUMO-3projections, sumoylation, INSDC_feature:centromere, chromatin modification, Procedures, SUMO-Conjugations, conformation, lamellae, SUMO-protein conjugation, Phases, Smt3h2, Gene, Sentrin-2, process of organ, protrusion, lamella, Mitotic M, reduced, chromatin organisation, Method, Sumoylations, establishment and maintenance of position, Studies, Gene Products, establishment and maintenance of substrate location, tiny, small ubiquitin-related protein 1 conjugation, Chromatins, pericentric region, study, M Phases, Centromeres, hypoplasia, proteins, chromatid, protein sumolation, procedures, ridges, ESI, SUMO-2, SUMO-3, Study, centromere, centric region, Methodological Studies, papilla, chromosome scaffold, associated, laminae, relational structural quality, small, close to, Gene., establishment and maintenance of substance location, anatomical protrusion, establishment or maintenance of chromatin architecture, Smt3b, anatomical process, Proteins, lamina, flanges, Procedure, HSMT3, results, near to, polypeptide, prophase chromosome, single organism localization, mitosis, Protein, shelf, Mitotic M Phase, establishment and maintenance of localization, techniques, SMT3 homolog 2, regulator, M Phase, SMT3 homolog 1, centromere complex, flange, organ process, Ubiquitin, Phase, Mitoses, underdeveloped, interphase chromosome, shelves, Kinetochore, single-organism localization, Ubiquitin-like protein SMT3B, Methodological, Methodological Study, projection, ridge, SUMO Conjugation, Protein Gene Products, Gene Proteins, process, processes, localisation, chromosome, Ubiquitin-like protein SMT3A, Chromosome, spine, approaches, Smt3A, Smt3B, vicinity of, Mitotic M Phases, processus, Ubiquitin-related, Mitotic, SUMO-Conjugation, establishment and maintenance of cellular component location, methodology04http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005148dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0massive~1hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0varsite~00Resource Reanalysis0.00.00.00.01.0falseIdentification of SUMO-2/3 modified proteins associated with mitotic chromosomes.Data from Massive, ID: MSV000078890, PXD ID: PXD001534. File: MM_8A2_SUMO23_mitosis_C_75KDa_v1.mzml. Published as part of Proteomics. 2014 Nov 4 . From the Abstract: {{i}} ... In this study, we used chromosome spreads to more precisely define the localization of SUMO-2/3 to the inner-centromere and protein scaffold of mitotic chromosomes. We also developed methods to immunopurify proteins modified by endogenous, untagged SUMO-2/3 from mitotic chromosomes ... {{/i}}2014-11-24GPM3231000514825367092MSV000078890