{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":17,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Huesgen PF, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Homo_sapiens_viruses, Human_female"],"submitter_mail":["p.huesgen@fz-juelich.de"],"publication":["25419962"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005426","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005427","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005468","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005424","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005425","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005469","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005428","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005429","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005462","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005463","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005460","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005461","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005466","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005422","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005423","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005467","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005464","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005420","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005421","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005465","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005470","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005415","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005459","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005416","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005413","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005457","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005458","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005414","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005419","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005417","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005418","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005451","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005452","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005450","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005455","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005412","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005456","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005453","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005448","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005449","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005446","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005447","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005440","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005441","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005444","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005445","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005442","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005443","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005437","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005438","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005435","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005436","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005439","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005430","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005471","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005433","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005434","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005431","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005432"],"submitter_affiliation":["UBC"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["To improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 °C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion-dominated spectra. This improved protein C terminal-peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications."],"pubmed_title":["LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification."],"pubmed_authors":["Huesgen Pitter F PF,Lange Philipp F PF,Rogers Lindsay D LD,Solis Nestor N,Eckhard Ulrich U,Kleifeld Oded O,Goulas Theodoros T,Gomis-Rüth F Xavier FX,Overall Christopher M CM,","Huesgen Pitter F PF, Lange Philipp F PF, Rogers Lindsay D LD, Solis Nestor N, Eckhard Ulrich U, Kleifeld Oded O, Goulas Theodoros T, Gomis-Rüth F Xavier FX, Overall Christopher M CM"],"name_synonyms":["Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Peptide P-C, Parotid proline-rich protein 1|2, Proactivator polypeptide, Saposin-B-Val, Parotid double-band protein, Dispersin, beta-Trypsin, Pa, Tripcellim, Saposin-A, CSAct, Identifications (Psychology), SAP-1, Parotid isoelectric focusing variant protein, SAP-2, PRP-3|PRP-4, Co-beta-glucosidase, beta Trypsin, Identification., Trypure, Cerebroside sulfate activator, Db-s, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Parotid acidic protein, PIF-F, Sulfatide|GM1 activator, Sphingolipid activator protein 2, methylation, Db-F, PIF-S, A1 activator, Methylations, Glucosylceramidase activator"],"description_synonyms":["6)36(30, 7a, Lysine Hydrochloride, 7b, 35-, DL-Arginine Acetate, fractured, Parotid double-band protein, Pa, L Isomer, cleavage, 27-28, Monohydrate, 7b-dihydroxy-3-(hydroxymethyl)-1, 12-O-Tetradecanoylphorbol 13-acetate, 8R, 4]benzo[1, 1bS, peptide, Polypeptides, Db-s, Lysine Acetate, 1-6H3/t24-, tetradecanoylphorbol acetate, Parotid acidic protein, Epigenomic., 1, 2, 4, ruptured, 5, Lysine, 6, Sphingolipid activator protein 2, 8, 9R, 9, A1 activator, InChIKey=PHEDXBVPIONUQT-RGYGYFBIBK, L Lysine, 12-Tetradecanoylphorbol 13-acetate, 24, Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, 4aR, 2-e]azulen-9-yl tetradecanoate, Identification, C36H56O8, Saposin-B-Val, Dispersin, 1b, InChI=1/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5, beta-Trypsin, [H][C@@]12C=C(CO)C[C@]3(O)C(=O)C(C)=C[C@@]3([H])[C@@]1(O)[C@H](C)[C@@H](OC(=O)CCCCCCCCCCCCC)[C@]1(OC(C)=O)[C@@]2([H])C1(C)C, epsilon-diaminocaproic acid, proteasome endopeptidase activity, Arginine, SAP-1, Monohydrate DL-Arginine Acetate, SAP-2, C6H14N2O2, 30, 32, 36-/m1/s1, 37, 7-18, Component C, Lysin, PIF-F, 9a-decahydro-1H-cyclopropa[3, 30-, PIF-S, data, 27+, elastase activity, 7aS, Peptide P-C, Proactivator polypeptide, Arginine Hydrochloride, split, Saposin-A, Parotid isoelectric focusing variant protein, 8-tetramethyl-5-oxo-1a, PRP-3|PRP-4, Co-beta-glucosidase, L-Isomer, DL Arginine Acetate, fragmented, polypeptide, Experiment, 28-, 7bS, L-Isomer Arginine, Acetate, 4a, endoprotease activity, 32-, Hydrochloride, hemorrhaged, 9aS)-9a-acetoxy-4a, L Arginine, 41-42H, L-Lysine, burst, phorbol 12-tetradecanoate 13-acetate, L-Arginine, torn, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, (1aR, Sphingolipid activator protein 1, Epigenetic, Parotid proline-rich protein 1|2, cracked, Tripcellim, CSAct, Identifications (Psychology), alpha, beta Trypsin, 21-22H2, Trypure, Cerebroside sulfate activator, PMA, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, 34-, Sulfatide|GM1 activator, proteinase, Db-F, 32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20, Epigenetics, 6-diaminohexanoic acid, Proteomes, Enisyl, Glucosylceramidase activator"],"pubmed_title_synonyms":["Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Peptide P-C, Parotid proline-rich protein 1|2, Proactivator polypeptide, Saposin-B-Val, Parotid double-band protein, Dispersin, beta-Trypsin, Pa, Tripcellim, Saposin-A, CSAct, Identifications (Psychology), SAP-1, Parotid isoelectric focusing variant protein, SAP-2, PRP-3|PRP-4, Co-beta-glucosidase, beta Trypsin, Identification., Trypure, Cerebroside sulfate activator, Db-s, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Parotid acidic protein, PIF-F, Sulfatide|GM1 activator, Sphingolipid activator protein 2, methylation, Db-F, PIF-S, A1 activator, Methylations, Glucosylceramidase activator"],"pubmed_abstract_synonyms":["elastase activity, Lysine Hydrochloride, Peptide P-C, Proactivator polypeptide, DL-Arginine Acetate, fractured, Parotid double-band protein, Pa, Arginine Hydrochloride, L Isomer, cleavage, split, Saposin-A, Monohydrate, Parotid isoelectric focusing variant protein, PRP-3|PRP-4, Co-beta-glucosidase, L-Isomer, DL Arginine Acetate, fragmented, peptide, polypeptide, Polypeptides, Db-s, Lysine Acetate, L-Isomer Arginine, Acetate, endoprotease activity, Parotid acidic protein, Epigenomic., Hydrochloride, hemorrhaged, 2, ruptured, Lysine, L Arginine, Sphingolipid activator protein 2, L-Lysine, A1 activator, burst, L Lysine, L-Arginine, torn, Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Identification, Epigenetic, Parotid proline-rich protein 1|2, Saposin-B-Val, cracked, Dispersin, beta-Trypsin, Tripcellim, epsilon-diaminocaproic acid, CSAct, Identifications (Psychology), proteasome endopeptidase activity, Arginine, SAP-1, Monohydrate DL-Arginine Acetate, SAP-2, alpha, C6H14N2O2, beta Trypsin, Trypure, Cerebroside sulfate activator, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Lysin, PIF-F, Sulfatide|GM1 activator, proteinase, Db-F, Epigenetics, 6-diaminohexanoic acid, PIF-S, Proteomes, Enisyl, Glucosylceramidase activator"],"view_count":["17"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005457"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.005243676742751388"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.","description":"Data from ProteomeXchange: PXD ID: PXD001114. Experiment: LysargiNase, silac, file: LR_Ulilysin_PMA_A1_121126.mzml. Published as part of  . From the Abstract: {{i}} To improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion-dominated spectra. This improved protein C terminal-peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications. {{/i}}","dates":{"submission":"2014-12-17"},"accession":"GPM32310005457","cross_references":{"pubmed":["25419962"],"Pride":["PXD001114"],"pride":[],"Pride Archive":["PXD001114"]}}