GPMDB00175OtherHuesgen PF, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_femalep.huesgen@fz-juelich.de25419962http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005426http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005427http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005468http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005424http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005425http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005469http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005428http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005429http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005462http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005463http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005460http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005461http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005466http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005422http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005423http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005467http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005464http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005420http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005421http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005465http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005470http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005415http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005459http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005416http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005413http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005457http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005458http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005414http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005419http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005417http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005418http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005451http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005452http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005450http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005455http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005412http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005456http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005453http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005448http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005449http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005446http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005447http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005440http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005441http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005444http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005445http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005442http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005443http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005437http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005438http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005435http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005436http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005439http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005430http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005471http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005433http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005434http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005431http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005432UBCNot availableGPMDBTo improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 °C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion-dominated spectra. This improved protein C terminal-peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications.LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.Huesgen Pitter F PF,Lange Philipp F PF,Rogers Lindsay D LD,Solis Nestor N,Eckhard Ulrich U,Kleifeld Oded O,Goulas Theodoros T,Gomis-Rüth F Xavier FX,Overall Christopher M CM,Huesgen Pitter F PF, Lange Philipp F PF, Rogers Lindsay D LD, Solis Nestor N, Eckhard Ulrich U, Kleifeld Oded O, Goulas Theodoros T, Gomis-Rüth F Xavier FX, Overall Christopher M CMSalivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Peptide P-C, Parotid proline-rich protein 1|2, Proactivator polypeptide, Saposin-B-Val, Parotid double-band protein, Dispersin, beta-Trypsin, Pa, Tripcellim, Saposin-A, CSAct, Identifications (Psychology), SAP-1, Parotid isoelectric focusing variant protein, SAP-2, PRP-3|PRP-4, Co-beta-glucosidase, beta Trypsin, Identification., Trypure, Cerebroside sulfate activator, Db-s, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Parotid acidic protein, PIF-F, Sulfatide|GM1 activator, Sphingolipid activator protein 2, methylation, Db-F, PIF-S, A1 activator, Methylations, Glucosylceramidase activator6)36(30, 7a, Lysine Hydrochloride, 7b, 35-, DL-Arginine Acetate, fractured, Parotid double-band protein, Pa, L Isomer, cleavage, 27-28, Monohydrate, 7b-dihydroxy-3-(hydroxymethyl)-1, 12-O-Tetradecanoylphorbol 13-acetate, 8R, 4]benzo[1, 1bS, peptide, Polypeptides, Db-s, Lysine Acetate, 1-6H3/t24-, tetradecanoylphorbol acetate, Parotid acidic protein, Epigenomic., 1, 2, 4, ruptured, 5, Lysine, 6, Sphingolipid activator protein 2, 8, 9R, 9, A1 activator, InChIKey=PHEDXBVPIONUQT-RGYGYFBIBK, L Lysine, 12-Tetradecanoylphorbol 13-acetate, 24, Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, 4aR, 2-e]azulen-9-yl tetradecanoate, Identification, C36H56O8, Saposin-B-Val, Dispersin, 1b, InChI=1/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5, beta-Trypsin, [H][C@@]12C=C(CO)C[C@]3(O)C(=O)C(C)=C[C@@]3([H])[C@@]1(O)[C@H](C)[C@@H](OC(=O)CCCCCCCCCCCCC)[C@]1(OC(C)=O)[C@@]2([H])C1(C)C, epsilon-diaminocaproic acid, proteasome endopeptidase activity, Arginine, SAP-1, Monohydrate DL-Arginine Acetate, SAP-2, C6H14N2O2, 30, 32, 36-/m1/s1, 37, 7-18, Component C, Lysin, PIF-F, 9a-decahydro-1H-cyclopropa[3, 30-, PIF-S, data, 27+, elastase activity, 7aS, Peptide P-C, Proactivator polypeptide, Arginine Hydrochloride, split, Saposin-A, Parotid isoelectric focusing variant protein, 8-tetramethyl-5-oxo-1a, PRP-3|PRP-4, Co-beta-glucosidase, L-Isomer, DL Arginine Acetate, fragmented, polypeptide, Experiment, 28-, 7bS, L-Isomer Arginine, Acetate, 4a, endoprotease activity, 32-, Hydrochloride, hemorrhaged, 9aS)-9a-acetoxy-4a, L Arginine, 41-42H, L-Lysine, burst, phorbol 12-tetradecanoate 13-acetate, L-Arginine, torn, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, (1aR, Sphingolipid activator protein 1, Epigenetic, Parotid proline-rich protein 1|2, cracked, Tripcellim, CSAct, Identifications (Psychology), alpha, beta Trypsin, 21-22H2, Trypure, Cerebroside sulfate activator, PMA, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, 34-, Sulfatide|GM1 activator, proteinase, Db-F, 32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20, Epigenetics, 6-diaminohexanoic acid, Proteomes, Enisyl, Glucosylceramidase activatorSalivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Peptide P-C, Parotid proline-rich protein 1|2, Proactivator polypeptide, Saposin-B-Val, Parotid double-band protein, Dispersin, beta-Trypsin, Pa, Tripcellim, Saposin-A, CSAct, Identifications (Psychology), SAP-1, Parotid isoelectric focusing variant protein, SAP-2, PRP-3|PRP-4, Co-beta-glucosidase, beta Trypsin, Identification., Trypure, Cerebroside sulfate activator, Db-s, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Parotid acidic protein, PIF-F, Sulfatide|GM1 activator, Sphingolipid activator protein 2, methylation, Db-F, PIF-S, A1 activator, Methylations, Glucosylceramidase activatorelastase activity, Lysine Hydrochloride, Peptide P-C, Proactivator polypeptide, DL-Arginine Acetate, fractured, Parotid double-band protein, Pa, Arginine Hydrochloride, L Isomer, cleavage, split, Saposin-A, Monohydrate, Parotid isoelectric focusing variant protein, PRP-3|PRP-4, Co-beta-glucosidase, L-Isomer, DL Arginine Acetate, fragmented, peptide, polypeptide, Polypeptides, Db-s, Lysine Acetate, L-Isomer Arginine, Acetate, endoprotease activity, Parotid acidic protein, Epigenomic., Hydrochloride, hemorrhaged, 2, ruptured, Lysine, L Arginine, Sphingolipid activator protein 2, L-Lysine, A1 activator, burst, L Lysine, L-Arginine, torn, Salivary acidic proline-rich phosphoprotein 1|2, Pr1|Pr2, PRP-1|PRP-2, Salivary acidic proline-rich phosphoprotein 3|4, Sphingolipid activator protein 1, Identification, Epigenetic, Parotid proline-rich protein 1|2, Saposin-B-Val, cracked, Dispersin, beta-Trypsin, Tripcellim, epsilon-diaminocaproic acid, CSAct, Identifications (Psychology), proteasome endopeptidase activity, Arginine, SAP-1, Monohydrate DL-Arginine Acetate, SAP-2, alpha, C6H14N2O2, beta Trypsin, Trypure, Cerebroside sulfate activator, Component C, Saposin-D, Protein C, Saposin-C, Protein A, Saposin-B, Lysin, PIF-F, Sulfatide|GM1 activator, proteinase, Db-F, Epigenetics, 6-diaminohexanoic acid, PIF-S, Proteomes, Enisyl, Glucosylceramidase activator1705http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310005457dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0052436767427513880.01.0falseLysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.Data from ProteomeXchange: PXD ID: PXD001114. Experiment: LysargiNase, silac, file: LR_Ulilysin_PMA_A1_121126.mzml. Published as part of . From the Abstract: {{i}} To improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion-dominated spectra. This improved protein C terminal-peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications. {{/i}}2014-12-17GPM3231000545725419962PXD001114PXD001114