{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":67,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Handtke S, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Crap, Bacillus_pumilus_safr_032"],"submitter_mail":["hecker@uni-greifswald.de"],"publication":["25281541"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018340","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018339","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018338","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018331","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018298","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018297","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018330","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018333","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018299","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018332","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018335","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018334","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018337","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018336","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018290","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018292","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018291","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018294","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018293","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018296","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018295","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018328","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018327","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018329","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018320","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018287","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018286","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018289","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018322","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018321","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018288","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018324","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018323","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018326","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018325","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018360","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018362","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018361","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018353","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018352","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018355","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018354","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018357","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018356","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018359","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018358","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018351","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018350","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018349","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018342","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018341","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018344","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018343","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018346","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018345","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018348","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018347","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018380","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018382","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018381","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018384","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018383","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018375","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018374","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018377","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018376","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018379","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018378","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018371","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018370","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018373","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018372","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018364","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018363","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018366","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018365","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018368","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018401","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018400","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018367","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018369","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018402","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018283","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018285","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018284","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018317","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018316","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018319","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018318","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018397","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018396","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018311","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018399","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018310","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018398","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018313","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018312","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018315","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018314","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018391","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018390","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018393","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018392","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018395","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018394","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018306","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018305","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018308","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018307","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018309","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018386","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018385","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018300","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018387","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018302","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018389","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018301","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018304","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018303"],"submitter_affiliation":["Institute for Microbiology, Ernst-Moritz-Arndt University, Greifswald, et al."],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed."],"pubmed_title":["Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-an omics-based approach."],"pubmed_authors":["Handtke Stefan S,Volland Sonja S,Methling Karen K,Albrecht Dirk D,Becher Dörte D,Nehls Jenny J,Bongaerts Johannes J,Maurer Karl-Heinz KH,Lalk Michael M,Liesegang Heiko H,Voigt Birgit B,Daniel Rolf R,Hecker Michael M,","Handtke Stefan S, Volland Sonja S, Methling Karen K, Albrecht Dirk D, Becher Dörte D, Nehls Jenny J, Bongaerts Johannes J, Maurer Karl-Heinz KH, Lalk Michael M, Liesegang Heiko H, Voigt Birgit B, Daniel Rolf R, Hecker Michael M"],"name_synonyms":["Bacillus aminoglucosidicus., cell physiology, cell growth and/or maintenance, cellular physiological process, single-organism cellular process"],"description_synonyms":["Gene., membrane, data, Sectors, exocrine gland fluid, determination, GLM2, exocrine gland fluid or secretion, Lipoprotein, external secretion, Tertiary Sectors, Sprain, Proteins, nutrient medium, Gene, Profiles, Circulating, membranous organ component, Cell, Bacillus aminoglucosidicus, strain, exocrine gland fluid/secretion, polypeptide, MHAM, Experiment, GMCSFR, Tertiary, PTEN1, GMR, CWS1, chemical analysis, Protein, Strain, Gene Products, secretion, cultivar, TEP1, CSF2RAY, Sprains, Metabolic Profile, SMDP4, CSF2RAX, Metabolic Profiles, Industries, study, membrane of organ, FOCUS, 10q23del, Circulating Lipoproteins, Metabolic, medium, Metabolomes, growth medium, MMAC1, Profile, proteins, CD116, ecotype, CSF2R, Protein Gene Products, Gene Proteins, DEC, secreted substance, CSF2RX, CSF2RY, Sector, CDw116, bodily secretion, GM-CSF-R-alpha, Lipoproteins, Tertiary Sector, species, Strains, assay, BZS, Strains and Sprains, exocrine gland secretion, Proteomes"],"pubmed_title_synonyms":["Bacillus aminoglucosidicus., cell physiology, cell growth and/or maintenance, cellular physiological process, single-organism cellular process"],"pubmed_abstract_synonyms":["membrane, Sectors, Alpha-TAT, TAT, exocrine gland fluid, Procedures, determination, exocrine gland fluid or secretion, Lipoprotein, external secretion, Alpha-TAT1, Tertiary Sectors, Sprain, Proteins, nutrient medium, Gene, Profiles, Circulating, membranous organ component, Procedure, body system, Cell, predicted, Bacillus aminoglucosidicus, strain, exocrine gland fluid/secretion, polypeptide, Tertiary, Method, chemical analysis, Protein, Strain, Gene Products, Studies, SEC, sequence, secretion, system, cultivar, techniques, connected anatomical system, Sprains, Metabolic Profile, Metabolic Profiles, Genomes., Industries, study, membrane of organ, anatomical systems, FOCUS, Circulating Lipoproteins, Metabolic, Genomes, medium, Metabolomes, growth medium, MEC17, Profile, Selb, sec, proteins, whole genome, Methodological, procedures, Methodological Study, ecotype, primary structure of sequence macromolecule, Protein Gene Products, organ system, Study, Gene Proteins, Acetyltransferase mec-17 homolog, secreted substance, Nbla00487, Sector, Methodological Studies, bodily secretion, Lipoproteins, Tertiary Sector, species, Strains, assay, Strains and Sprains, exocrine gland secretion, Proteomes, C6orf134, 2.3.1.108, methodology"],"view_count":["67"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018327"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.02066625539790253"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-an omics-based approach.","description":"Data from ProteomeXchange, PXD ID: PXD000780. Experiment: O2_U1_gmr_sh_11_1, file: 100223_O2_U1_gmr_sh_11_1_4.mzml. Published as part of J Biotechnol. 2014 Dec 20;192 Pt A:204-14  . From the Abstract: {{i}} Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins ... {{/i}}","dates":{"submission":"2015-03-12"},"accession":"GPM32310018327","cross_references":{"pubmed":["25281541"],"Pride":["PXD000780"],"pride":[],"Pride Archive":["PXD000780"]}}