{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":42,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Hayes JA, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Bordetella_pertussis_tohama_i"],"submitter_mail":["frank.schmidt@uni-greifswald.de"],"publication":["25755163"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018526","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018525","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018522","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018521","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018524"],"submitter_affiliation":["CINDEFI (UNLP CONICET La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["One of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. This study reports a gel-free nanoLC-MS/MS-based comparative proteome analysis of Bordetella pertussis grown under iron-excess and iron-depleted conditions. Out of the 926 proteins covered 98 displayed a shift in their abundance in response to low iron availability. Forty-seven of them were found to be increased in level while 58 were found with decreased protein levels under iron starvation. In addition to proteins previously reported to be influenced by iron in B. pertussis, we observed changes in metabolic proteins involved in fatty acid utilization and poly-hydroxybutyrate production. Additionally, many bacterial virulence factors regulated by the BvgAS two-component system were found at decreased levels in response to iron limitation. These results, together with the increased production of proteins potentially involved in oxidative stress resistance, seem to indicate that iron starvation provokes changes in B. pertussis phenotype that might shape host-pathogen interaction."],"pubmed_title":["Shotgun proteome analysis of Bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response."],"pubmed_authors":["Alvarez Hayes Jimena J,Lamberti Yanina Y,Surmann Kristin K,Schmidt Frank F,Völker Uwe U,Rodriguez Maria Eugenia ME,","Alvarez Hayes Jimena J, Lamberti Yanina Y, Surmann Kristin K, Schmidt Frank F, Völker Uwe U, Rodriguez Maria Eugenia ME"],"name_synonyms":["biochemical pathways, incorporation, Metabolic Process, Haemophilus pertussis, Bacterium tussis-convulsivae, [Fe], determination, degradation, Process, InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG, defense/immunity protein activity., Processes, metabolism resulting in cell growth, Metabolic Concepts, fer, Metabolic Processes, antimicrobial peptide activity, Concept, Metabolic Phenomena, Metabolism Concepts, Iron 56, Metabolism, chemical analysis, Phenomena, Pathogenicity, Concepts, defence response, 26Fe, Hemophilus pertussis, Metabolism Concept, Phenomenon, metabolism, Metabolism Phenomena, Metabolic Phenomenon, viral infection, hierro, ferrum, mobilization, multicellular organism metabolic process, physiological defense response, distinct, biodegradation, Metabolic, availability, catabolism, InChI=1/Fe, Metabolic Concept, metabolic process resulting in cell growth, virus process, Iron-56, turnover, Eisen, single-organism metabolic process, biotransformation, virulence, assay, iron atom, Catabolism, Proteomes, Microbe de la coqueluche, Anabolism"],"description_synonyms":["study, host organism, InChI=1/Fe., data, hierro, ferrum, Haemophilus pertussis, Bacterium tussis-convulsivae, [Fe], IRF-1, determination, InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG, InChI=1/Fe, fer, Iron-56, free, Eisen, Iron 56, chemical analysis, 26Fe, assay, Hemophilus pertussis, iron atom, Proteomes, MAR, Microbe de la coqueluche"],"pubmed_title_synonyms":["biochemical pathways, incorporation, Metabolic Process, Haemophilus pertussis, Bacterium tussis-convulsivae, [Fe], determination, degradation, Process, InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG, defense/immunity protein activity., Processes, metabolism resulting in cell growth, Metabolic Concepts, fer, Metabolic Processes, antimicrobial peptide activity, Concept, Metabolic Phenomena, Metabolism Concepts, Iron 56, Metabolism, chemical analysis, Phenomena, Pathogenicity, Concepts, defence response, 26Fe, Hemophilus pertussis, Metabolism Concept, Phenomenon, metabolism, Metabolism Phenomena, Metabolic Phenomenon, viral infection, hierro, ferrum, mobilization, multicellular organism metabolic process, physiological defense response, distinct, biodegradation, Metabolic, availability, catabolism, InChI=1/Fe, Metabolic Concept, metabolic process resulting in cell growth, virus process, Iron-56, turnover, Eisen, single-organism metabolic process, biotransformation, virulence, assay, iron atom, Catabolism, Proteomes, Microbe de la coqueluche, Anabolism"],"pubmed_abstract_synonyms":["acido graso, protein levels, host organism, polynuclear neutrophilic leukocyte, Virulence, Haemophilus pertussis, Bacterium tussis-convulsivae, acidos grasos, parasitism, [Fe], determination, pertussis, Gene, Pertusses, commensalism, Bordetella pertussis Infection, supernumerary, body system, Stresses, neutrophilic leucocyte, reduced, acides gras, relational shape quality, Oxidative, subnumerary, responsivity, neutrophil leukocyte, Famines, resistance, Gene Products, system, 26Fe, encompassing mutualism through parasitism, Low, symbiosis, present in fewer numbers in organism, study, reactivity, increased, hierro, me75, anatomical systems, symbiotic interaction between host and organism, availability, Whooping, InChI=1/Fe, neutrophil leucocyte, Famine, proteins, mature neutrocyte, neutrophilic leukocyte, decreased number, free, D17Mit170, T1, Pathogenicity Factors, decreased, mature neutrophilic leucocyte, Oxidative Stresses, Respiratory, OC([*])=O, polynuclear neutrophilic leucocyte, polymorphonuclear leukocyte, iron atom, Microbe de la coqueluche, symbiotic interaction between organisms, use, cou, mature neutrophil leucocyte, InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG, Proteins, Pertussis, fer, Fettsaeuren, Tl3, Tl2, neutrophils, results, polypeptide, Fettsaeure, neutrocyte, Lr, Iron 56, chemical analysis, Protein, Pathogenicity, Hemophilus pertussis, connected anatomical system, Starvations, CHO2R, ferrum, Factors, mature neutrophil leukocyte, increased number, symbiotic interaction, acide gras, mature neutrophilic leukocyte, Iron-56, Protein Gene Products, organ system, host-pathogen interaction, Gene Proteins, present in greater numbers in organism, Phenotypes, fatty acids, Eisen, symbiotic interaction between species., Cough, Stress, Bra, PMN, polymorphonuclear leucocyte, response to iron, assay, response, poly, Proteomes, polymorphonuclear neutrophil, accessory"],"view_count":["42"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310018525"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.012954966070326958"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Shotgun proteome analysis of Bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response.","description":"Data from ProteomeXchange, PXD ID: PXD001280. File: 120313_O4_YL__Bp_Fe_1.mzml. Published as part of Proteomics. 2015 Mar 9  . From the Abstract: {{i}} One of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. This study reports a gel-free nanoLC-MS/MS-based comparative proteome analysis of Bordetella pertussis grown under iron-excess and iron-depleted conditions ... {{/i}}","dates":{"submission":"2015-03-17"},"accession":"GPM32310018525","cross_references":{"pubmed":["25755163"],"Pride":["PXD001280"],"pride":[],"Pride Archive":["PXD001280"]}}