GPMDB00125OtherAlpert AJ, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_femalehudecz@imp.univie.ac.at25827581http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019291http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019290http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019293http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019292http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019295http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019294http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019297http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019296http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019307http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019306http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019309http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019308http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019321http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019288http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019287http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019320http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019323http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019301http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019300http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019322http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019303http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019325http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019302http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019324http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019305http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019327http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019304http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019326http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019286http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019318http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019317http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019319http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019310http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019299http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019298http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019312http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019311http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019314http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019313http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019316http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019315Institute of Molecular Biotechnology of the Austrian Academy of Sciences, et al.Not availableGPMDBMost phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained in AEX. However, peptides with one or two phosphate groups are not separated from peptides with multiple Asp or Glu residues, interfering with the identification of phosphopeptides. At a pH of 2, phosphate residues have just a single negative charge but Asp and Glu are uncharged. This facilitates the separation of phosphopeptides from unmodified acidic peptides. Singly phosphorylated peptides are retained weakly under these conditions, due to electrostatic repulsion, unless hydrophilic interaction is superimposed in the ERLIC mode. Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared, with both peptide standards and a HeLa cell tryptic digest. The SAX column exhibited greater retention at pH 6 than did the WAX column. However, only about 60% as many phosphopeptides were identified with SAX at pH 6 than via ERLIC at pH 2. In one ERLIC run, 12 467 phosphopeptides were identified, including 4233 with more than one phosphate. We conclude that chromatography of phosphopeptides is best performed at low pH in the ERLIC mode. Under those conditions, the performances of the SAX and WAX materials were comparable. The data have been deposited with the ProteomeXchange with identifier PXD001333.Anion-exchange chromatography of phosphopeptides: weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography.Alpert Andrew J AJ,Hudecz Otto O,Mechtler Karl K,Alpert Andrew J AJ, Hudecz Otto O, Mechtler Karl Kstrong, Chromatographies, resilient, Chromatographies., tough, weakIGF-I, APR, column., data, SeptD1, Stars, steel factor, criteria, JCAP, LRP, Slf, 6.3.2.-, Slpa, hematopoietic growth factor KL, RING finger protein 52, RNF52, Eseptin, Somatomedin-C, FNZ, somatomedin, APOER, FPH2, FINC, HAPO, guidelines, LETS, sKITLG, CIG, peptide, polypeptide, SINT1, CD91, resilient, Ms1, IMP, mechano growth factor, tough, TGFBR5, PNUTL4, ED-B, Sint1, HELA cell, IMPA, Igf-1, AF17q25, NAPB, strong, SHEP7, SZP, FN, mast cell growth factor, maintenance of localization, MS1, storage, MS2, NOXA, Stem cell factor, weak, HeLa, Striated muscle activator of Rho-dependent signaling, E430039A18Rik, somatomedin-C, SF, CACP, Kitl, STAT5, CD156a, Impedes mitogenic signal propagation, BRAP2, Mast cell growth factor, CD156, retention, MSF, Msf, Renal carcinoma antigen NY-REN-63, 5'-inosinic acid, stem cell factor, KL-1, A2MR, column, IGF1, MGF, c-Kit ligand, Mechano growth factor, LRP1A, KITLG, GFND2, GFND, MSF1, Soluble KIT ligand, sequestering, SCF, STARS, Sl, IGFBP3Rstrong, Chromatographies, resilient, Chromatographies., tough, weakcriteria, ARMD9, C3a, Glu, C3b, APG5L, splitted from, Spectrum Analyses, Complement C3 beta chain, guidelines, Complement C3 alpha chain, limitations, Complement C3b alpha' chain, peptide, Polypeptides, APG5, Calmbp1, resilient, tough, Mass, ARB, Low, Analysis, study limitations, Mass Spectroscopy, Complement C3d fragment, Mass Spectrum Analysis, study, strong, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, me75, Acylation stimulating protein, PPG, 1810073H04Rik, Analyses, Identification, VMD2, weak, HeLa, Agouti coat color protein, As, Complement C3dg fragment, BMD, CPAMD1, D17Mit170, T1, ASP, retention, C3adesArg, ACF, Acf, AHUS5, column, Complement C3f fragment, discontiguous, data., Complement C3c alpha' chain fragment 1, Chromatographies, Complement C3c alpha' chain fragment 2, hAPG5, RP50, APG5-LIKE, AI255234, cou, C3bc, ACF65, Liquid Chromatography, ACF64, Tl3, A-lt-y-gt-, Tl2, Spectrum Analysis, Spectroscopy, APOBEC1CF, polypeptide, Lr, divided_from, materials, HELA cell, TU15B, MCPH5, Mass Spectrum Analyses, Mass Spectrum, CHARGES, ASIP, maintenance of localization, storage, C3a anaphylatoxin, Spectrometry, Identifications (Psychology), Plp, Agouti switch protein, C3-beta-c, GLP-1, HEL-S-62p, HSE-MSF, Bra, Complement C3g fragment, phosphates, sequestering, BEST1205http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310019320dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0037014188772362740.01.0falseAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion-Hydrophilic Interaction Chromatography.Data from ProteomeXchange, PXD ID: PXD001333. File: Amanda ms1-8ppm ms2-20ppm 20130219_QEx3_RSLC-4_Mischerikow_Peters_IMP_shotgun_s2324.msf.mgf. Published as part of Anal Chem. 2015 Apr 17 . From the Abstract: {{i}} ... Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared, with both peptide standards and a HeLa cell tryptic digest. The SAX column exhibited greater retention at pH 6 than did the WAX column. However, only about 60% as many phosphopeptides were identified with SAX at pH 6 than via ERLIC at pH 2 ... {{/i}}2015-04-24GPM3231001932025827581PXD001333PXD001333