GPMDB00415OtherAeberhard L, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_female, Chlamydia_trachomatis_l2b_uch_1_proctitis, Chlamydia_trachomatis_l2clukas.aeberhard@gmx.net26042774http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020391http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020392http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020393http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020394http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020390http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020399http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020410http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020411http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020400http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020412http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020401http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020413http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020402http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020395http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020396http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020397http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020398http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020407http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020408http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020409http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020404http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020405http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020406Robert Koch Institute, Berlin, et al.Not availableGPMDBChlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. Computational analysis indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells' antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular, SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, these findings suggest that C. trachomatis hijacks retrograde pathways for effective infection.The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components.Aeberhard Lukas L,Banhart Sebastian S,Fischer Martina M,Jehmlich Nico N,Rose Laura L,Koch Sophia S,Laue Michael M,Renard Bernhard Y BY,Schmidt Frank F,Heuer Dagmar D,Aeberhard Lukas L, Banhart Sebastian S, Fischer Martina M, Jehmlich Nico N, Rose Laura L, Koch Sophia S, Laue Michael M, Renard Bernhard Y BY, Schmidt Frank F, Heuer Dagmar DRickettsia trachomatis, Chlamydozoon trachomatis, Rickettsia trachomae, Vacuole., vacuolar carboxypeptidase Y, Proteomesmembrane, data, Chlamydia Infection, human being, nucleocytoplasm, Rickettsiaformis, vacuolar carboxypeptidase Y, Vacuole, AP-1, c-jun, number, Chlamydia, biosynthesis, Homo sapiense, membranous organ component, presence, Chlamydozoon trachomatis, Rickettsia trachomae, Homo spaiens, count in organism, Homo sapien, count, Homo sapian, Homo sapians, Rickettsia trachomatis, Infection, chlamydial disease, Miyagawanella, Homo sapeins, internal to cell, Rakeia, multicellular organismal biosynthetic process, protoplasm, Chlamydial Infection, Junc, single-organism biosynthetic process, Homo sapients, membrane of organ, protoplast, Infections, formation, anabolism, Humo sapiens, Chlamydia Infections, man, Homo sapines, human, Chlamydophila, synthesis, AP1, Homo spiens, presence or absence in organism., "human" EXACT genbank_common_name [], Homo sapience, Homo sampiens, c-Jun, quantitative, Bedsonia, Home sapiens, ProteomesRickettsia trachomatis, Chlamydozoon trachomatis, Rickettsia trachomae, Vacuole., vacuolar carboxypeptidase Y, ProteomesSorting Nexin, screening, membrane, host organism, Chlamydia Infection, AU019504, human being, nucleocytoplasm, Rickettsiaformis, findings, HRR1, vacuolar carboxypeptidase Y, determination, Vacuole, number, Chlamydia, biosynthesis, Homo sapiense, membranous organ component, presence, Nexin, Cell, Chlamydozoon trachomatis, Rickettsia trachomae, Homo spaiens, count in organism, BETA2AR, RNF47, Homo sapien, conclusions, count, Homo sapian, ADRBR, Nexins, Homo sapians, chemical analysis, Rickettsia trachomatis, Infection, SYNEXIN, symptoms, HRR-1, chlamydial disease, concluding remarks, Miyagawanella, Homo sapeins, internal to cell, Rakeia, multicellular organismal biosynthetic process, protoplasm, Chlamydial Infection, FXR, single-organism biosynthetic process, Homo sapients, membrane of organ, protoplast, Infections, formation, anabolism, conclusion, ANX7, D2Ertd52e, Humo sapiens, signs, Chlamydia Infections, Infections., man, Homo sapines, SNX, human, Chlamydophila, synthesis, summary, Homo spiens, "human" EXACT genbank_common_name [], Homo sapience, 0910001N05Rik, Homo sampiens, 1810032P22Rik, Sorting, BAR, assay, quantitative, Bedsonia, RIP14, Home sapiens, Proteomes, ADRB2R, presence or absence in organism, B2AR4105http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310020401dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0126465144972239370.01.0falseThe Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components.Data from ProteomeXchange, PXD ID: PXD002113. File: 130514_LA_C_92_SAX8.mzml. Published as part of PLoS Pathog. 2015 Jun 4;11(6):e1004883 . From the Abstract: {{i}} Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. ... {{/i}}2015-06-08GPM3231002040126042774PXD002113PXD002113