GPMDB00445OtherHongbo Guo, et al.InstrumentNot AvailableNot availableStrongylocentrotus_purpuratushongboguo88@gmail.com26227301University of Torontohttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023131http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023098http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023010http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023130http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023097http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023012http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023133http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023132http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023011http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023099http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023094http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023093http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023096http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023095http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023018http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023139http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023138http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023017http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023019http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023014http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023135http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023134http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023013http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023016http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023137http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023136http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023015http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023021http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023142http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023020http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023141http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023023http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023144http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023022http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023143http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023140http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023029http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023028http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023149http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310022976http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023146http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023025http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023024http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023145http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023027http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023148http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023026http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023147http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310022977http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310022978http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310022979http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023076http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023075http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023078http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023111http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023110http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023077http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023072http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023071http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023074http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023073http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023117http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023116http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023119http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023118http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023113http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023112http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023079http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023115http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023114http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023081http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023080http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023087http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023120http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023086http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023001http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023089http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023122http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023121http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023000http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023088http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32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availableGPMDBFertilization triggers a dynamic symphony of molecular transformations induced by a rapid rise in intracellular calcium. Most prominent are surface alterations, metabolic activation, cytoskeletal reorganization, and cell-cycle reentry. While the activation process appears to be broadly evolutionarily conserved, and protein phosphorylation is known to play a key role, the signaling networks mediating the response to fertilization are not well described. To address this gap, we performed a time course phosphoproteomic analysis of egg activation in the sea urchin Strongylocentrotus purpuratus, a system that offers biochemical tractability coupled with exquisite synchronicity. By coupling large-scale phosphopeptide enrichment with unbiased quantitative MS, we identified striking changes in global phosphoprotein patterns at 2- and 5-min postfertilization as compared to unfertilized eggs. Overall, we mapped 8796 distinct phosphosite modifications on 2833 phosphoproteins, of which 15% were differentially regulated in early egg activation. Activated kinases were identified by phosphosite mapping, while enrichment analyses revealed conserved signaling cascades not previously associated with egg activation. This work represents the most comprehensive study of signaling associated with egg activation to date, suggesting novel mechanisms that can be experimentally tested and providing a valuable resource for the broader research community. All MS data have been deposited in the ProteomeXchange with identifier PXD002239 (http://proteomecentral.proteomexchange.org/dataset/PXD002239).Phosphoproteomic network analysis in the sea urchin Strongylocentrotus purpuratus reveals new candidates in egg activation.Guo Hongbo H,Garcia-Vedrenne Ana Elisa AE,Isserlin Ruth R,Lugowski Andrew A,Morada Anthony A,Sun Alex A,Miao Yishen Y,Kuzmanov Uros U,Wan Cuihong C,Ma Hongyue H,Foltz Kathy K,Emili Andrew A,Guo Hongbo H, Garcia-Vedrenne Ana Elisa AE, Isserlin Ruth R, Lugowski Andrew A, Morada Anthony A, Sun Alex A, Miao Yishen Y, Kuzmanov Uros U, Wan Cuihong C, Ma Hongyue H, Foltz Kathy K, Emili Andrew APurple Sea Urchins, purple sea urchin, network topology analysis, Purple Sea Urchin, Purple., SLC20A3, Urchin, Strongylocentrotus purpuratus (Stimpson, Sea Urchins, 1857), SEA, Purple Sea, Sea Urchin, Urchins, Purple, D2L2AD, CTP, purple urchinprojections, Conceptions, biological signaling, Plays, nucleocytoplasm, Urchin, Polyspermic Fertilization, Sea Urchins, Effects, lamellae, Calcium 40, GTPase-activating protein, ras p21 protein activator, process of organ, Long Term, protrusion, Delayed Fertilizations, lamella, protein amino acid phosphorylation, period, surface, Roles, 20Ca, Factor IV, responsivity, Cycle, SEA, Concepts, PKWS, system, Effect, Polyspermic, integumentum commune, Toy, protoplasm, Cell Division Cycles, Cycles, calcio, reactivity, Calcium-40, anatomical systems, protoplast, Playthings, RasGAP, Longterm, Cell Division Cycle, cell-division cycle, Urchins, Cell Division, ridges, Long-Term, purple urchin, Puppets, Delayed Fertilization, papilla, signaling process, Cell Cycles, Role Concepts, Play, CM-AVM, Long-Term Effect, laminae, Long-Term Effects, Puppet, Blood Coagulation Factor IV, Ca, single organism signaling, data, Purple Sea Urchin, Coagulation, anatomical protrusion, Polyspermic Fertilizations, external covering of organism, 1857), anatomical process, lamina, Longterm Effect, flanges, organism surface, Kalzium, RASGAP, D2L2AD, RASA, Cell, Division Cycles, Concept, Experiment, Role Concept, HCD, Conception, Strongylocentrotus purpuratus (Stimpson, Playthings and Play, body system., Long Term Effects, shelf, proteomic analysis, Role, Plaything, RASA1, internal to cell, GAP, connected anatomical system, p120GAP, p120RASGAP, activation, flange, organ process, Calcium, SLC20A3, Division Cycle, body surface, shelves, Purple Sea, Toys, CMAVM, projection, ridge, signalling, Longterm Effects, organ system, process, processes, Purple Sea Urchins, Delayed, purple sea urchin, Fertilization, Coagulation Factor IV, signalling process, integumentary system, spine, syngamy, Fertilizations, Ras p21 protein activator, Sea Urchin, processus, Purple, response, dermal system, time, CTPPurple Sea Urchins, purple sea urchin, network topology analysis, Purple Sea Urchin, Purple., SLC20A3, Urchin, Strongylocentrotus purpuratus (Stimpson, Sea Urchins, 1857), SEA, Purple Sea, Sea Urchin, Urchins, Purple, D2L2AD, CTP, purple urchinprojections, Conceptions, scale tissue, nucleocytoplasm, Polyspermic Fertilization, Activity, determination, Sea Urchins, Laboratory, Unfertilized Egg, Calcium 40, ras p21 protein activator, Long Term, Delayed Fertilizations, Ccne, Roles, responsivity, SEA, Concepts, PKWS, Research Activity, Gpi-1, Effect, Laboratory Research, integumentum commune, Priorities, calcio, Nlk, plant peltate hair, Urchins, min, W, purple urchin, papilla, signaling process, Role Concepts, CM-AVM, Research Priority, associated, Long-Term Effects, Blood Coagulation Factor IV, Ca, single organism signaling, Data Set., Gpi, Coagulation, anatomical protrusion, Polyspermic Fertilizations, external covering of organism, 1857), lamina, Longterm Effect, flanges, Research Priorities, organism surface, RASA, Role Concept, count, mOC-X, Playthings and Play, shelf, Role, precocious, Plaything, NK|GPI, internal to cell, GAP, p120RASGAP, activation, Research and Development, Org, ORG, SLC20A3, shelves, Purple Sea, Toys, expanded, Gpi1-r, Gpi1-s, Gpi1-t, projection, ridge, early, organ system, Activities, Purple Sea Urchins, Delayed, purple sea urchin, Fertilization, enlarged, integumentary system, spine, syngamy, Ras p21 protein activator, Sea Urchin, Purple, dermal system, time, Bglap-rs1, Gpi1s, big, biological signaling, advanced, Plays, Urchin, Effects, AI461847, lamellae, Eggs, peltate hair, number, GTPase-activating protein, process of organ, body system, presence, CYCLE, protrusion, Unfertilized Eggs, lamella, protein amino acid phosphorylation, period, surface, large, 20Ca, Factor IV, system, Pgi, Ova, Polyspermic, Toy, protoplasm, Unfertilized, reactivity, study, Calcium-40, anatomical systems, protoplast, Playthings, RasGAP, Longterm, Research, cell, Gpi-1r, Gpi-1s, Phi, Gpi-1t, ridges, Long-Term, Puppets, Delayed Fertilization, great, Play, Long-Term Effect, Development and Research, laminae, Puppet, data, Purple Sea Urchin, anatomical process, Kalzium, MF, RASGAP, D2L2AD, Amf, Cell, Concept, count in organism, Priority, Conception, Strongylocentrotus purpuratus (Stimpson, Long Term Effects, chemical analysis, Research Activities, RASA1, connected anatomical system, scales, p120GAP, flange, organ process, NK, Calcium, scale, distinct, body surface, CMAVM, signalling, Longterm Effects, phosphorylated protein, process, processes, Egg, Coagulation Factor IV, signalling process, Fertilizations, processus, assay, Prot/PhosRes+, response, quantitative, CTP, presence or absence in 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archive~1varsite~00Resource Reanalysis0.00.00.0135718692165330040.01.0falsePhosphoproteomic Network Analysis in the Sea Urchin Strongylocentrotus purpuratus Reveals New Candidates in Egg Activation.Data from ProteomeXchange, PXD ID: PXD002239. Experiment: HILIC_hcd_seaurchin_UN3, file: HILIC_hcd_seaurchin_UN3_F14.mzml. Published as part of Proteomics. 2015 Jul 29 . From the Abstract: {{i}} Fertilization triggers a dynamic symphony of molecular transformations induced by a rapid rise in intracellular calcium. Most prominent are surface alterations, metabolic activation, cytoskeletal reorganization,and cell cycle reentry. While the activation process appears to be broadly evolutionarily conserved, and protein phosphorylation is known to play a key role, the signaling networks mediating the response to fertilization are not well described. To address this gap, we performed a time course phospho proteomic analysis of egg activation in the sea urchin Strongylocentrotus purpuratus, a system that offers biochemical tractability coupled with exquisite synchronicity. {{/i}}2015-08-08GPM3231002314126227301PXD002239PXD002239