GPMDB00135OtherZhang Y, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanhefc@nic.bmi.ac.cn26282447http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023493http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023496http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023495http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023490http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023492http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023491http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023513http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023512http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023515http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023514http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023498http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023531http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023530http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023497http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023511http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023510http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023499http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023517http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023516http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023519http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023518http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023524http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023502http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023523http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023501http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023504http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023526http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023525http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023503http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023520http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023500http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023522http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023489http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023488http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023521http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023509http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023528http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023506http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023505http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023527http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023508http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023507http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023529Beijing Proteome Research CenterNot availableGPMDBInvestigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Proteins were extracted and separated with glycine- and tricine-SDS-PAGE. A total of 9597 protein groups were identified; of these, 166 protein groups were listed as MPs, including 138 groups (83.1%) with transcriptional evidence. A total of 2948 proteins are designated as MPs, and 5.6% of these were identified in this study. The high incidence of MPs in testis tissue indicates that this is a rich resource for MPs. Functional category analysis revealed that the biological processes that testis MPs are mainly involved in are sexual reproduction and spermatogenesis. Some of the MPs are potentially involved in tumorgenesis in other tissues. Therefore, this proteogenomics analysis of individual testis tissues provides convincing evidence of the discovery of MPs. All mass spectrometry data from this study have been deposited in the ProteomeXchange (data set identifier PXD002179).Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins.Zhang Yao Y,Li Qidan Q,Wu Feilin F,Zhou Ruo R,Qi Yingzi Y,Su Na N,Chen Lingsheng L,Xu Shaohang S,Jiang Tao T,Zhang Chengpu C,Cheng Gang G,Chen Xinguo X,Kong Degang D,Wang Yujia Y,Zhang Tao T,Zi Jin J,Wei Wei W,Gao Yuan Y,Zhen Bei B,Xiong Zhi Z,Wu Songfeng S,Yang Pengyuan P,Wang Quanhui Q,Wen Bo B,He Fuchu F,Xu Ping P,Liu Siqi S,Zhang Yao Y, Li Qidan Q, Wu Feilin F, Zhou Ruo R, Qi Yingzi Y, Su Na N, Chen Lingsheng L, Xu Shaohang S, Jiang Tao T, Zhang Chengpu C, Cheng Gang G, Chen Xinguo X, Kong Degang D, Wang Yujia Y, Zhang Tao T, Zi Jin J, Wei Wei W, Gao Yuan Y, Zhen Bei B, Xiong Zhi Z, Wu Songfeng S, Yang Pengyuan P, Wang Quanhui Q, Wen Bo B, He Fuchu F, Xu Ping P, Liu Siqi SGene., Profiling, human being, reproductive system of male organism gonad, testicle, Researchs, gonada of male genitals, Proteins, male organism reproductive system gonad, gonad of male organism reproductive system, Proteogenomic Profiling, Homo sapiense, gonad of male genitals, genitalia of male organism gonad, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, polypeptide, Homo spaiens, male genitalia gonada, Homo sapien, male genitals gonad, Homo sapian, gonad of genitalia of male organism, male reproductive system gonad, Homo sapians, Protein, gonad of male genitalia, Gene Products, tissue portion, simple tissue, Proteogenomic Research, Analysis, testiculus, Homo sapeins, genitalia of male organism gonada, gonada of reproductive system of male organism, Entire testis, portion of tissue, Homo sapients, Research, gonada of male reproductive system, Testicle, gonada of genitalia of male organism, gonada of male organism reproductive system, Proteogenomic Analysis, Tissue, Humo sapiens, male gonad, gonad of male organism genitalia, proteins, man, Homo sapines, gonad of reproductive system of male organism, human, male reproductive system gonada, Protein Gene Products, Gene Proteins, Homo spiens, "human" EXACT genbank_common_name [], Proteogenomic, Homo sapience, Homo sampiens, orchis, Testicles, male genitalia gonad, male organism reproductive system gonada, testes, gonada of male genitalia, male organism genitalia gonada, male organism genitalia gonad, Home sapiens, TestesGlycine <subgenus>, deceased, Glycine Carbonate (2:1), human being, Calcium Salt Glycine, Sodium Hydrogen Carbonate, testicle, Slf, Researchs, male organism reproductive system gonad, Somatomedin-C, Proteogenomic Profiling, Gene, FPH2, glycine, Cardiac Death, Monoammonium, Homo sapiense, Monosodium, gonada of male organism genitalia, GLYCINE, gonad of male reproductive system, reproductive system of male organism gonada, SDS, male genitalia gonada, Homo spaiens, Homo sapien, Calcium Salt (2:1), male reproductive system gonad, Homo sapians, gonad of male genitalia, Gene Products, Monoammonium Salt Glycine, testiculus, Analysis, Glycine Hydrochloride (2:1), Separated, portion of tissue, Divorced, Homo sapients, Gene Expressions, Glycine Hydrochloride, Research, Testicle, Glycine, gonada of genitalia of male organism, gonada of male organism reproductive system, Tissue, Monosodium Salt, somatomedin-C, SF, gonad of male organism genitalia, 4733401P19Rik, Kitl, proteins, Divorces, Phosphate, Expressions, man, gonad of reproductive system of male organism, Mast cell growth factor, Salt Glycine, Proteogenomic, Homo sapience, IGF1, orchis, Homo sampiens, male genitalia gonad, Mechano growth factor, Monopotasssium Salt, 2-aminoacetic acid, Soluble KIT ligand, Expression, testes, male organism genitalia gonada, Sl, Home sapiens, IGF-I, Monoammonium Salt, Profiling, Monopotasssium Salt Glycine, Cobalt Salt Glycine, data, Glycine Phosphate (1:1), steel factor, N-(2-hydroxy-1, reproductive system of male organism gonad, Glycine Phosphate, hematopoietic growth factor KL, gonada of male genitals, Proteins, gonad of male organism reproductive system, Copper Salt, somatomedin, AI836084, Monosodium Salt Glycine, gonad of male genitals, genitalia of male organism gonad, sKITLG, Pancreatic insufficiency and bone marrow dysfunction., Copper Salt Glycine, polypeptide, Experiment, Glycine Carbonate (1:1), male genitals gonad, gonad of genitalia of male organism, Homo sapian, mechano growth factor, Protein, post-mortem, Hydrochloride, tissue portion, Proteogenomic Research, simple tissue, Homo sapeins, Igf-1, Death, genitalia of male organism gonada, gonada of reproductive system of male organism, 1-bis(hydroxymethyl)ethyl)glycine, Entire testis, Acid, Separation, Aminoacetic Acid, SHEP7, mast cell growth factor, gonada of male reproductive system, Glycine Sulfate (3:1), Stem cell factor, Proteogenomic Analysis, Separations, Humo sapiens, male gonad, SWDS, Monopotassium Salt, Shwachman-Bodian-Diamond syndrome, Cobalt Salt, Shwachman syndrome, Monolithium Salt, STAT5, CGI-97, Homo sapines, human, male reproductive system gonada, Protein Gene Products, Gene Proteins, stem cell factor, Homo spiens, KL-1, "human" EXACT genbank_common_name [], Aminoacetic, MGF, Testicles, c-Kit ligand, KITLG, aminoacetic acid, male organism reproductive system gonada, gonada of male genitalia, SCF, male organism genitalia gonad, Proteomes, Testes, Calcium SaltGene., Profiling, human being, reproductive system of male organism gonad, testicle, Researchs, gonada of male genitals, Proteins, male organism reproductive system gonad, gonad of male organism reproductive system, Proteogenomic Profiling, Homo sapiense, gonad of male genitals, genitalia of male organism gonad, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, polypeptide, Homo spaiens, male genitalia gonada, Homo sapien, male genitals gonad, Homo sapian, gonad of genitalia of male organism, male reproductive system gonad, Homo sapians, Protein, gonad of male genitalia, Gene Products, tissue portion, simple tissue, Proteogenomic Research, Analysis, testiculus, Homo sapeins, genitalia of male organism gonada, gonada of reproductive system of male organism, Entire testis, portion of tissue, Homo sapients, Research, gonada of male reproductive system, Testicle, gonada of genitalia of male organism, gonada of male organism reproductive system, Proteogenomic Analysis, Tissue, Humo sapiens, male gonad, gonad of male organism genitalia, proteins, man, Homo sapines, gonad of reproductive system of male organism, human, male reproductive system gonada, Protein Gene Products, Gene Proteins, Homo spiens, "human" EXACT genbank_common_name [], Proteogenomic, Homo sapience, Homo sampiens, orchis, Testicles, male genitalia gonad, male organism reproductive system gonada, testes, gonada of male genitalia, male organism genitalia gonada, male organism genitalia gonad, Home sapiens, TestesGlycine <subgenus>, projections, deceased, Glycine Carbonate (2:1), Spermiogenesis, human being, Calcium Salt Glycine, Sodium Hydrogen Carbonate, determination, testicle, Researchs, Incidences, lamellae, male organism reproductive system gonad, Proteogenomic Profiling, Gene, glycine, Cardiac Death, Monoammonium, Spectrum Analyses, Homo sapiense, Monosodium, process of organ, temporal, gonada of male organism genitalia, protrusion, GLYCINE, gonad of male reproductive system, reproductive system of male organism gonada, lamella, SDS, male genitalia gonada, Homo spaiens, Homo sapien, Calcium Salt (2:1), male reproductive system gonad, Homo sapians, gonad of male genitalia, Gene Products, Monoammonium Salt Glycine, Mass, Analysis, testiculus, Glycine Hydrochloride (2:1), Separated, Mass Spectroscopy, Mass Spectrum Analysis, portion of tissue, study, Divorced, Homo sapients, Gene Expressions, Glycine Hydrochloride, Analyses, Research, Testicle, Glycine, gonada of genitalia of male organism, gonada of male organism reproductive system, Tissue, Monosodium Salt, gonad of male organism genitalia, 4733401P19Rik, proteins, Divorces, Phosphate, ridges, Expressions, man, gonad of reproductive system of male organism, Salt Glycine, Proteogenomic, Homo sapience, orchis, Homo sampiens, papilla, male genitalia gonad, Monopotasssium Salt, 2-aminoacetic acid, Expression, testes, male organism genitalia gonada, laminae, Home sapiens, incidence, Data Set., Monoammonium Salt, Profiling, Monopotasssium Salt Glycine, Cobalt Salt Glycine, data, Glycine Phosphate (1:1), anatomical protrusion, N-(2-hydroxy-1, reproductive system of male organism gonad, Glycine Phosphate, anatomical process, Proteins, gonada of male genitals, gonad of male organism reproductive system, lamina, Copper Salt, AI836084, flanges, Spermatocytogenesis, Monosodium Salt Glycine, gonad of male genitals, Spectrum Analysis, genitalia of male organism gonad, Copper Salt Glycine, Spectroscopy, polypeptide, Glycine 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Aminoacetic, Testicles, processus, aminoacetic acid, assay, male organism reproductive system gonada, gonada of male genitalia, male organism genitalia gonad, Testes, Calcium Salt1305http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310023526dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0040098704503392970.01.0falseThe Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins.Data from ProteomeXchange, PXD ID: PXD002179. Experiment: CHPP_TESTIS_Tricine, file: CHPP_TESTIS_Tricine_2017.mgf. Published as part of J Proteome Res. 2015 . From the Abstract: {{i}} Investigations of missing proteins (MPs) are being endorsed by many bioanalytical strategies. We proposed that proteogenomics of testis tissue was a feasible approach to identify more MPs because testis tissues have higher gene expression levels. Here, we combined proteomics and transcriptomics to survey gene expression in human testis tissues from three post-mortem individuals. Protein were extracted and separated with glycine- and tricine-SDS-PAGE ... {{/i}}2015-09-01GPM3231002352626282447PXD002179PXD002179