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of Biological Sciences, Nanyang Technological University, et al."],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Extracellular vesicles (EVs) such as exosomes and microvesicles mediate intercellular communication and regulate a diverse range of crucial biological processes. Host cells that are damaged, infected or transformed release biomarker-containing EVs into the peripheral circulation, where they can be readily accessed for use in diagnostic or prognostic testing. However, current methods of EV isolation from blood plasma are complex and often require relatively large sample volumes, hence are inefficient for widespread use in clinical settings. Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lipid-encapsulated EVs behind in suspension. This generates higher purity EVs that can then be obtained from filtration or classical ultracentrifugation methods. We foresee that PROSPR-based purification of EVs will significantly accelerate the discovery of new disease biomarkers and the characterization of EVs with potential for clinical applications."],"pubmed_title":["Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR)."],"pubmed_authors":["Gallart-Palau Xavier X,Serra Aida A,Wong Andrew See Weng AS,Sandin Sara S,Lai Mitchell K P MK,Chen Christopher P CP,Kon Oi Lian OL,Sze Siu Kwan SK,","Gallart-Palau Xavier X, Serra Aida A, Wong Andrew See Weng AS, Sandin Sara S, Lai Mitchell K P MK, Chen Christopher P CP, Kon Oi Lian OL, Sze Siu Kwan SK"],"name_synonyms":["Plasma, Fresh, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, portion of blood plasma, Homo sapients, Frozen Plasma, human being, Exovesicles, Fresh Frozen Plasma, Apoptotic Body, Bodies, Blood, Apoptotic Bodies, Vesicles, Humo sapiens, portion of plasma, Extracellular Vesicle, Homo sapiense, man, Homo sapines, Plasmas, Body, human, Vesicle, Frozen Plasmas, polypeptide, proteins., Homo spaiens, blood plasm, Homo spiens, \"human\" EXACT genbank_common_name [], Homo sapien, Homo sapience, Homo sapian, Homo sampiens, Extracellular, Homo sapians, Blood Plasmas, Apoptotic, Homo sapeins, Home sapiens, Exovesicle, plasma"],"description_synonyms":["projections, host organism, PLXN5, Blood, Chronic Airflow Obstructions, Nl1, Extracellular Vesicle, Homo sapiense, SeP, 2-Propanone, Personal, CEH, Homo sapien, Communications, Method, Homo sapians, chronic obstructive airways disease NOS (disorder), Chronic irreversible airway obstruction, NEPII, NL1, NL2, SEH, Chronic Obstructive Pulmonary Disease (COPD), methyl ketone, SEP, Fresh Frozen Plasmas, Fresh Frozen, Homo sapients, COLD (chronic obstructive lung disease), NEC in ICD9CM_2006, Personnel, PTPSTEP, hypoplasia, CAFL - Chronic airflow limitation, purification, proteins, procedures, Chronic airflow limitation, sEP, Communication, isolation and purification, Methodological Studies, Homo sapience, Propanon, papilla, sample, Home sapiens, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, REP, plasma, CHRONIC, chronic obstructive lung disease [Ambiguous], Frozen Plasma, anatomical protrusion, Lipids., lamina, flanges, Procedure, Plasmas, Airflow Obstructions, Aceton, InChI=1/C3H6O/c1-3(2)4/h1-2H3, Programs, CHRONIC OBSTRUCTIVE AIRWAY DIS, Program, CC(C)=O, soluble, MMEL2, Homo sapian, low temperature, CHRONIC OBSTRUCTIVE, Apoptotic, shelf, 3.1.3.48, chronic obstructive pulmonary disease and allied conditions, Chronic airway disease, NOS, Airflow Obstruction, COPD NOS, CHRONIC OBSTRUCTIVE LUNG DIS, Chronic Obstructive Airways Disease, Chronic airway obstruction, Chronic obstructive lung disease, portion of blood plasma, cold, Step, shelves, expanded, Striatum-enriched protein-tyrosine phosphatase, Methodological, study protocol, projection, Methodological Study, Homo sapines, ridge, human, Chronic Airflow Obstruction, blood plasm, enlarged, spine, STEP, OBSTRUCTIVE PULMONARY DISEASE (COPD), COPD - Chronic obstructive pulmonary disease, Eph2, Communication Program, COLD, big, Fresh, Misinformation, Rab interactor activity, human being, Exovesicles, Procedures, Fresh Frozen Plasma, Apoptotic Body, Bodies, lamellae, DISEASE (COPD), CAO - Chronic airflow obstruction, Vesicles, InChIKey=CSCPPACGZOOCGX-UHFFFAOYAF, Gene, PLEXIN-B1, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, C3H6O, process of organ, protrusion, Chronic obstructive pulmonary disease finding, lamella, large, method, Homo spaiens, propanone, PULMONARY DISEASE (COPD), Pulmonary Disease, reduced, isolation, method used in an experiment, dimethylcetone, Studies, Gene Products, tiny, propan-2-one, Blood Plasma, ridges, Chronic Obstructive, man, Communication Programs, Study, Neural-specific protein-tyrosine phosphatase, Homo sampiens, Extracellular, great, Blood Plasmas, NEC, SELP, Chronic, biological marker, Azeton, laminae, Dimethyl ketone, COPD, Personal Communication, small, data, COAD - Chronic obstructive airways disease, Dimethylketon, use, chronic obstructive airways disease NOS, anatomical process, not elsewhere classified, Apoptotic Bodies, Proteins, COAD, Mell1, COLD - Chronic obstructive lung disease, Cell, PULM DIS CHRONIC OBSTRUCTIVE, Chronic obstructive pulmonary disease finding (finding), Vesicle, polypeptide, Frozen Plasmas, Rab escort protein activity, Communications Personnel, Protein, techniques, (COPD), Homo sapeins, chronic airway obstruction, Exovesicle, flange, CAL - Chronic airflow limitation, organ process, chronic obstructive airway disease, Plasma, underdeveloped, Dops, Humo sapiens, portion of plasma, Body, sample population, plan specification, Protein Gene Products, process, processes, Gene Proteins, Homo spiens, \"human\" EXACT genbank_common_name [], chronic obstructive lung disease (disorder), processus, Chronic Obstructive Lung Disease, NEP2, methodology"],"pubmed_title_synonyms":["Plasma, Fresh, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, portion of blood plasma, Homo sapients, Frozen Plasma, human being, Exovesicles, Fresh Frozen Plasma, Apoptotic Body, Bodies, Blood, Apoptotic Bodies, Vesicles, Humo sapiens, portion of plasma, Extracellular Vesicle, Homo sapiense, man, Homo sapines, Plasmas, Body, human, Vesicle, Frozen Plasmas, polypeptide, proteins., Homo spaiens, blood plasm, Homo spiens, \"human\" EXACT genbank_common_name [], Homo sapien, Homo sapience, Homo sapian, Homo sampiens, Extracellular, Homo sapians, Blood Plasmas, Apoptotic, Homo sapeins, Home sapiens, Exovesicle, plasma"],"pubmed_abstract_synonyms":["projections, host organism, Viral Marker, Biological Markers, Surrogate Endpoints, Laboratory, Blood, Chronic Airflow Obstructions, Biochemical, Endpoint, Extracellular Vesicle, Homo sapiense, Serum, 2-Propanone, Personal, Laboratory Markers, Homo sapien, Communications, Method, Biological, Homo sapians, chronic obstructive airways disease NOS (disorder), Chronic irreversible airway obstruction, Chronic Obstructive Pulmonary Disease (COPD), methyl ketone, Fresh Frozen Plasmas, Fresh Frozen, human disease, Homo sapients, COLD (chronic obstructive lung disease), NEC in ICD9CM_2006, Personnel, PTPSTEP, hypoplasia, CAFL - Chronic airflow limitation, purification, proteins, procedures, Chronic airflow limitation, Communication, isolation and purification, Immune, Markers, Methodological Studies, Homo sapience, Propanon, papilla, Viral Markers, sample, Homo sapiens disease, Home sapiens, CHRONIC OBSTRUCTIVE PULMONARY DISEASE, plasma, CHRONIC, chronic obstructive lung disease [Ambiguous], Frozen Plasma, anatomical protrusion, Viral, Surrogate Endpoint, lamina, flanges, Biochemical Markers, Procedure, Plasmas, Biologic Marker, Airflow Obstructions, Aceton, InChI=1/C3H6O/c1-3(2)4/h1-2H3, Programs, CHRONIC OBSTRUCTIVE AIRWAY DIS, Program, CC(C)=O, soluble, Homo sapian, Marker, low temperature, CHRONIC OBSTRUCTIVE, Apoptotic, shelf, 3.1.3.48, chronic obstructive pulmonary disease and allied conditions, Diseases, Chronic airway disease, NOS, Airflow Obstruction, COPD NOS, CHRONIC OBSTRUCTIVE LUNG DIS, Chronic Obstructive Airways Disease, Chronic airway obstruction, Chronic obstructive lung disease, portion of blood plasma, Lipids, End Points, cold, Step, shelves, expanded, Striatum-enriched protein-tyrosine phosphatase, Methodological, Immunologic, Laboratory Marker, study protocol, projection, Methodological Study, Homo sapines, ridge, human, Chronic Airflow Obstruction, blood plasm, enlarged, spine, STEP, OBSTRUCTIVE PULMONARY DISEASE (COPD), Biochemical Marker, COPD - Chronic obstructive pulmonary disease, Communication Program, COLD, big, Fresh, Misinformation, human being, Exovesicles, Procedures, Fresh Frozen Plasma, Apoptotic Body, Bodies, Clinical Markers, lamellae, DISEASE (COPD), Clinical Marker, CAO - Chronic airflow obstruction, Vesicles, InChIKey=CSCPPACGZOOCGX-UHFFFAOYAF, Gene, CHRONIC OBSTRUCTIVE PULM DIS, Chronic obstructive pulmonary disease NOS, C3H6O, process of organ, protrusion, Surrogate End Points, Chronic obstructive pulmonary disease finding, lamella, Surrogate Markers, large, method, Homo spaiens, propanone, PULMONARY DISEASE (COPD), Pulmonary Disease, reduced, isolation, Filtrations, method used in an experiment, dimethylcetone, Studies, Gene Products, tiny, propan-2-one, Blood Plasma, Clinical, Biological Marker, ridges, Chronic Obstructive, man, Communication Programs, Study, Immunologic Markers, Neural-specific protein-tyrosine phosphatase, Homo sampiens, Extracellular, great, Blood Plasmas, NEC, Chronic, biological marker, Azeton, laminae, Dimethyl ketone, Immunologic Marker, Biologic, COPD, Personal Communication, small, COAD - Chronic obstructive airways disease, Dimethylketon, use, chronic obstructive airways disease NOS, anatomical process, not elsewhere classified, Apoptotic Bodies, Proteins, COAD, Serum Markers, End Point, COLD - Chronic obstructive lung disease, Cell, PULM DIS CHRONIC OBSTRUCTIVE, Chronic obstructive pulmonary disease finding (finding), Immune Marker, Vesicle, polypeptide, Frozen Plasmas, Surrogate End Point, Communications Personnel, Protein, techniques, (COPD), Homo sapeins, chronic airway obstruction, Viral., Exovesicle, flange, CAL - Chronic airflow limitation, organ process, Biologic Markers, chronic obstructive airway disease, Plasma, Serum Marker, underdeveloped, Surrogate, Dops, Endpoints, Humo sapiens, portion of plasma, Body, sample population, Surrogate Marker, plan specification, Protein Gene Products, process, processes, Gene Proteins, Homo spiens, \"human\" EXACT genbank_common_name [], chronic obstructive lung disease (disorder), processus, Chronic Obstructive Lung Disease, methodology, Immune Markers"],"view_count":["25"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32310024861"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.0077112893275755705"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR).","description":"Data from ProteomeXchange, PXD ID: PXD002668. File: UltraCushion_R1_28.mzml. Published as part of Sci Rep. 2015 Sep 30;5:14664  . From the Abstract: {{i}} Extracellular vesicles (EVs) such as exosomes and microvesicles mediate intercellular communication and regulate a diverse range of crucial biological processes. Host cells that are damaged, infected or transformed release biomarker-containing EVs into the peripheral circulation, where they can be readily accessed for use in diagnostic or prognostic testing. However, current methods of EV isolation from blood plasma are complex and often require relatively large sample volumes, hence are inefficient for widespread use in clinical settings. Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lipid-encapsulated EVs behind in suspension. {{/i}","dates":{"submission":"2015-10-06"},"accession":"GPM32310024861","cross_references":{"pubmed":["26419333"],"Pride":["PXD002668"],"pride":[],"Pride Archive":["PXD002668"]}}