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of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea, et al."],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Astrocytes are the most abundant cells in the CNS, but their function remains largely unknown. Characterization of the whole-cell proteome and secretome in astrocytes would facilitate the study of their functions in various neurodegenerative diseases and astrocyte-neuron communication. To build a reference proteome, we established a C8-D1A astrocyte proteome to a depth of 7265 unique protein groups using a novel strategy that combined two-step digestion, filter-aided sample preparation, StageTip-based high pH fractionation, and high-resolution MS. Nearly, 6000 unique protein groups were identified from conditioned media of astrocyte cultures, constituting the largest astrocyte secretome that has been reported. High-confidence whole-cell proteomes and secretomes are valuable resources in studying astrocyte function by label-free quantitation and bioinformatics analysis. All MS data have been deposited in the ProteomeXchange with identifier PXD000501 (http://proteomecentral.proteomexchange.org/dataset/PXD000501)."],"pubmed_title":["Proteomic analysis of mouse astrocytes and their secretome by a combination of FASP and StageTip-based, high pH, reversed-phase fractionation."],"pubmed_authors":["Han Dohyun D,Jin Jonghwa J,Woo Jongmin J,Min Hophil H,Kim Youngsoo Y,","Han Dohyun D, Jin Jonghwa J, Woo Jongmin J, Min Hophil H, Kim Youngsoo Y"],"name_synonyms":["Mus musculus, 3.4.22.-, Caspase-14 subunit p10, Laboratory, Swiss, mice, Mus domesticus, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, backward, \"mouse\" EXACT common_name [], MICE, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], reversed., House, Mus, Astrocyte, Astroglias, Mini-ICE, proteomic analysis, Mus musculus domesticus, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Astroglia, Mouse, house mouse, Mice, Laboratory Mouse"],"description_synonyms":["Personal Communication, Brain, Misinformation, C8-D1A, APR, data, astrocytic glia, Neurologic Disorder, nerve cell, neuraxis, Set1, LRP, Neurologic Diseases, Degenerative Neurologic Disorders, APOER, function, Neurologic Degenerative Condition, Cell, brain structure, central nervous system degenerative disorder, Programs, Neurodegenerative Diseases, Degenerative Neurologic Disorder, polypeptide, Program, Personal, Experiment, CD91, Communications, Neurologic Disorders, neuron cell, Astrocyte, Astroglias, cerebrospinal axis, Communications Personnel, central nervous system, TGFBR5, Set1A, synganglion, 3.1.3.48., Nervous System Degenerative Diseases, Cerebrospinal axis, study, Degenerative Conditions, KMT2F, Nervous System, Degenerative Condition, CNS, systema nervosum centrale, Degenerative Diseases, Step, cell, Personnel, NOXA, PTPSTEP, Neurologic Degenerative Conditions, proteins, Striatum-enriched protein-tyrosine phosphatase, Neurologic Disease, Neuraxis, Neurodegenerative Disorder, Neurodegenerative Disorders, Communication, Communication Programs, Neurologic, Entire central nervous system, Neurologic Degenerative Diseases, neuronal cell, Neural-specific protein-tyrosine phosphatase, A2MR, Degenerative Neurologic Disease, Degenerative, STEP, LRP1A, Degenerative Neurologic Diseases, Neurologic Degenerative Disease, Neurodegenerative Disease, Astroglia, Central Nervous System, Spinal Cord, Communication Program, Proteomes, IGFBP3R"],"pubmed_title_synonyms":["Mus musculus, 3.4.22.-, Caspase-14 subunit p10, Laboratory, Swiss, mice, Mus domesticus, mouse, Swiss Mouse, House Mice, Swiss Mice, mini-ICE, Caspase-14 subunit p19, CASP-14, backward, \"mouse\" EXACT common_name [], MICE, House Mouse, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Laboratory Mice, domesticus, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], reversed., House, Mus, Astrocyte, Astroglias, Mini-ICE, proteomic analysis, Mus musculus domesticus, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Astroglia, Mouse, house mouse, Mice, Laboratory Mouse"],"pubmed_abstract_synonyms":["Personal Communication, Data Set., Brain, Gpi, Misinformation, C8-D1A, data, astrocytic glia, Neurologic Disorder, nerve cell, neuraxis, determination, AI461847, Neurologic Diseases, Degenerative Neurologic Disorders, function, Neurologic Degenerative Condition, MF, Amf, Cell, brain structure, central nervous system degenerative disorder, Programs, Neurodegenerative Diseases, Degenerative Neurologic Disorder, polypeptide, Program, Personal, Communications, mOC-X, Neurologic Disorders, neuron cell, Astrocyte, Astroglias, cerebrospinal axis, Communications Personnel, chemical analysis, central nervous system, 3.1.3.48, Pgi, synganglion, NK|GPI, Gpi-1, NK, Nervous System Degenerative Diseases, Cerebrospinal axis, study, Degenerative Conditions, Nervous System, Org, ORG, Degenerative Condition, CNS, systema nervosum centrale, Degenerative Diseases, Step, cell, Personnel, Gpi-1r, Nlk, Gpi-1s, PTPSTEP, Phi, Gpi-1t, Gpi1-r, Gpi1-s, Neurologic Degenerative Conditions, proteins, Striatum-enriched protein-tyrosine phosphatase, Neurologic Disease, Neuraxis, Neurodegenerative Disorder, Gpi1-t, Neurodegenerative Disorders, free, Communication, Communication Programs, Neurologic, Entire central nervous system, Neurologic Degenerative Diseases, neuronal cell, Neural-specific protein-tyrosine phosphatase, Degenerative Neurologic Disease, Degenerative, STEP, Degenerative Neurologic Diseases, Neurologic Degenerative Disease, Neurodegenerative Disease, Astroglia, Central Nervous System, Spinal Cord, Communication Program, assay, Proteomes, Bglap-rs1, Gpi1s"],"view_count":["46"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320000882"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride 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C8-D1A cells analyzed. Experiment: C8D1A_secretome_set1_2, file: 20130530_C8D1A_secretome_set1_F6_2.mzml. Published as part of Proteomics. 2014 Apr 17  . From the Abstract: {{i}} Astrocytes are the most abundant cells in the CNS, but their function remains largely unknown. Characterization of the whole-cell proteome and secretome in astrocytes would facilitate the study of their functions in various neurodegenerative diseases and astrocyte-neuron communication. To build a reference proteome, we established a C8-D1A astrocyte proteome to a depth of 7265 unique protein groups using a novel strategy that combined two-step digestion, filter-aided sample preparation, StageTip-based high pH fractionation, and high-resolution MS. {{/i}}","dates":{"submission":"2014-06-07"},"accession":"GPM32320000882","cross_references":{"pubmed":["24753479"],"Pride":["PXD000501"],"pride":[],"Pride Archive":["PXD000501"]}}