GPMDB00143OtherHamelin R, et al.InstrumentNot AvailableNot availableDesulfotomaculum_reducens_mi_1romain.hamelin@epfl.chNot availablehttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001785http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001763http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001762http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001787http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001765http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001786http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001764http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001767http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001789http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001788http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001766http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001768http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001781http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001780http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001761http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001760http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001752http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001751http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001754http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001776http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001775http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001753http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001734http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001778http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001777http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001755http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001733http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001736http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001758http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001735http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001757http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001779http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001790http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001792http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001791http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001738http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001737http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001759EPFL Proteomics Core FacilityNot availableGPMDBprojections, liquid chromatography tandem mass spectroscopy, Desulfotomaculum reducens strain MI-1, C.I. 77491, experimental, lamellae, number, Goal, Gene, process of organ, presence, cell associated, protrusion, lamella, surface, reduced, subnumerary, LC-MSMS, 2-hydroxypropanoate, red iron oxide, Gene Products, tetraoxosulfate(VI), tiny, present in fewer numbers in organism, integumentum commune, insoluble, Desulfotomaculum reducens str. MI-1, treatment, study, methods, LCMSMS, cell, experimental section, beta-Trypsin, hypoplasia, proteins, ridges, decreased number, Fast, extruding from, decreased, g, papilla, gamma-ferric oxide, disease management, LC-MS-MS., Gram Positive Bacteria, ferric oxide red, laminae, lactate, Electrons, Positron, small, LC-MS2, sulfate, data, anatomical protrusion, external covering of organism, anatomical process, exits through, Negatron, Proteins, lamina, LC-MS/MS, flanges, Negatrons, organism surface, Positrons, Cell, lepidocrocite, polypeptide, count in organism, ferric oxide hydrate, LC/MS/MS, count, soluble, hematite, tetraoxosulfate(2-), Protein, shelf, ferric oxide monohydrate, Fast Electron, Fast Electrons, flange, organ process, tetraoxidosulfate(2-), growth pattern, underdeveloped, maghemite, body surface, non-developmental growth, shelves, iron oxide, Tripcellim, Electron, exposed, projection, beta Trypsin, ridge, experimental procedures, Protein Gene Products, process, processes, extracellular, Gene Proteins, Trypure, spine, integumentary system, liquid chromatography-tandem mass spectroscopy, colcothar, processus, liquid chromatography tandem mass spectrometry, quantitative, dermal system, cell bound, Sulfate, Proteomes, presence or absence in organism140http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM323200017593dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~1lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.004318322023442320.01.0falseCharacterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducensData from ProteomeXchange, PXD ID: PXD001072. File: EV_130902_Qexactive_B03.mzml. From ProteomeXchange: {{i}} Desulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria is poorly understood. Here, we investigated Fe(III) reduction with lactate, a non-fermentable substrate, as the electron donor. Lactate consumption is concomitant to Fe(III) reduction, but does not support significant growth, suggesting that little energy can be conserved from this process and that it may occur fortuitously. D. reducens can reduce both soluble (Fe(III)-citrate) and insoluble (hydrous ferric oxide, HFO) Fe(III). Because physically inaccessible HFO was not reduced, we concluded that reduction requires direct contact under these experimental conditions. This implies the presence of a surface exposed reductase capable of transferring electrons from the cell to the extracellular electron acceptor. With the goal of identifying candidate Fe(III) reductases, we carried out an investigation of the surface proteome (surfaceome) of D. reducens. Cell surface exposed proteins were extracted by trypsin cell shaving or by lysozyme treatment, and analyzed by liquid chromatography-tandem mass spectrometry. {{/i}}2014-07-31GPM32320001759PXD001072PXD001072