GPMDB00195OtherGuldbrandsen A, et alInstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanfrode.berven@biomed.uib.no25038066http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001813http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001835http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001970University of Bergen, NorwayNot availableGPMDBIn this study, the human cerebrospinal fluid (CSF) proteome was mapped using three different strategies prior to Orbitrap LC-MS/MS analysis: SDS-PAGE and mixed mode reversed phase-anion exchange for mapping the global CSF proteome, and hydrazide-based glycopeptide capture for mapping glycopeptides. A maximal protein set of 3081 proteins (28,811 peptide sequences) was identified, of which 520 were identified as glycoproteins from the glycopeptide enrichment strategy, including 1121 glycopeptides and their glycosylation sites. To our knowledge, this is the largest number of identified proteins and glycopeptides reported for CSF, including 417 glycosylation sites not previously reported. From parallel plasma samples, we identified 1050 proteins (9739 peptide sequences). An overlap of 877 proteins was found between the two body fluids, whereas 2204 proteins were identified only in CSF and 173 only in plasma. All mapping results are freely available via the new CSF Proteome Resource (http://probe.uib.no/csf-pr), which can be used to navigate the CSF proteome and help guide the selection of signature peptides in targeted quantitative proteomics.In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR).Guldbrandsen Astrid A,Vethe Heidrun H,Farag Yehia Y,Oveland Eystein E,Garberg Hilde H,Berle Magnus M,Myhr Kjell-Morten KM,Opsahl Jill A JA,Barsnes Harald H,Berven Frode S FS,Guldbrandsen Astrid A, Vethe Heidrun H, Farag Yehia Y, Oveland Eystein E, Garberg Hilde H, Berle Magnus M, Myhr Kjell-Morten KM, Opsahl Jill A JA, Barsnes Harald H, Berven Frode S FSCerebro Spinal Fluid, CSF2, Fluids, molgramostin, Cerebrospinal Fluids, GM-CSF, spinal fluid, CSF, Colony-stimulating factor, Csfgm, Cerebro, Spinal Fluid, sargramostim, Cerebrospinal, Cerebro Spinal Fluids, sargramostim., Spinal Fluids, Molgramostin, Fluid, cerebral spinal fluid, Sargramostim, colony-stimulating factor, Cerebro Spinal, liquor cerebrospinalis, Proteomes, GMCSFLC-MS2, liquid chromatography tandem mass spectroscopy, Fresh, SLAP-2, data, Frozen Plasma, Fresh Frozen Plasma, Blood, Metrs, LC-MS/MS, backward, Plasmas, METRS, Frozen Plasmas, Polypeptides, LC/MS/MS, Experiment, LC-MSMS, Separated, C20orf156, Plasma, Fresh Frozen Plasmas, Fresh Frozen, Separation, Blood Plasma, portion of blood plasma, Divorced, LCMSMS, Separations, MRS, beta-Trypsin, Tripcellim, portion of plasma, SPG70, Divorces, beta Trypsin, sample population, SLAP2, Src-like adapter protein 2, Trypure, Mtrns, Deimos, blood plasm, column, Modulator of antigen receptor signaling, liquid chromatography-tandem mass spectroscopy, Blood Plasmas, sample, liquid chromatography tandem mass spectrometry, LC-MS-MS., MTRNS, MARS, plasma, Phobos, reversedCerebro Spinal Fluid, CSF2, Fluids, molgramostin, Cerebrospinal Fluids, GM-CSF, spinal fluid, CSF, Colony-stimulating factor, Csfgm, Cerebro, Spinal Fluid, sargramostim, Cerebrospinal, Cerebro Spinal Fluids, sargramostim., Spinal Fluids, Molgramostin, Fluid, cerebral spinal fluid, Sargramostim, colony-stimulating factor, Cerebro Spinal, liquor cerebrospinalis, Proteomes, GMCSFliquid chromatography tandem mass spectroscopy, glicoproteinas, Fresh, CSF2, human being, Fresh Frozen Plasma, determination, selection process, Blood, number, Gene, GM-CSF, Homo sapiense, presence, PHAPII, LC-MS-MS, Cerebrospinal, peptide, SDS, Polypeptides, Homo spaiens, Homo sapien, cerebral spinal fluid, Glycoprotein, template-activating factor I, glycosylation, glycoproteins, Glycosylation, Homo sapians, LC-MSMS, colony-stimulating factor, Gene Products, Cerebro Spinal, liquor cerebrospinalis, glycoproteine, GMCSF, glicoproteina, study, SET, Glykoprotein, Fresh Frozen Plasmas, Fresh Frozen, Blood Plasma, Homo sapients, LCMSMS, TAF-I, Protein Glycosylations, phosphatase 2A inhibitor I2PP2A, glycoproteines, molgramostin, 4733401P19Rik, proteins, number of, man, sargramostim, IGAAD, Molgramostin, Homo sapience, Homo sampiens, Blood Plasmas, has or lacks parts of type, Home sapiens, plasma, Glykoproteine, Neoglycoproteins, LC-MS2, Cerebro Spinal Fluid, Frozen Plasma, Protein Glycosylation, Proteins, LC-MS/MS, Fluids, AI836084, Cerebrospinal Fluids, Glycosylations, spinal fluid, extra or missing physical or functional parts, backward, Plasmas, results, Spinal Fluid, Cerebro Spinal Fluids, mereological quality, polypeptide, Frozen Plasmas, Spinal Fluids, count in organism, LC/MS/MS, Fluid, count, I-2PP2A, Homo sapian, Sargramostim, chemical analysis, Protein, overlap, Homo sapeins, Plasma, Epistemology, portion of blood plasma, Body Fluid, HLA-DR-associated protein II, Humo sapiens, SWDS, portion of plasma, Shwachman-Bodian-Diamond syndrome, CSF, Shwachman syndrome, inhibitor of granzyme A-activated DNase, Colony-stimulating factor, Csfgm, CGI-97, Cerebro, Homo sapines, human, Body, Protein Gene Products, Gene Proteins, blood plasm, Homo spiens, presence or absence in organism., "human" EXACT genbank_common_name [], liquid chromatography-tandem mass spectroscopy, Pancreatic insufficiency and bone marrow dysfunction, [glycoprotein], cardinality, liquid chromatography tandem mass spectrometry, assay, quantitative, Proteomes, reversed1905http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320001835dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0058605798889574340.01.0falseIn-depth characterization of the cerebrospinal fluid proteome displayed through the CSF Proteome Resource (CSF-PR).Data from ProteomeXchange, PXD ID: PXD000656. File: folder summary. Published as part of . From ProteomeXchange: {{i}} 40 uL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Sample was in-solution trypsin digested and peptides were fractionated into 10 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. {{/i}}2014-08-05GPM3232000183525038066PXD000656PXD000656