GPMDB00286OtherDephoure N, et al.InstrumentNot AvailableNot availableYeast25073701nod2007@med.cornell.eduWeill Cornell Medical College Department of Biochemistryhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002115http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002324http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002202http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002303http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002091http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002228http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002153http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002135http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002255Not availableGPMDBAneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms.Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.Dephoure Noah N,Hwang Sunyoung S,O'Sullivan Ciara C,Dodgson Stacie E SE,Gygi Steven P SP,Amon Angelika A,Torres Eduardo M EM,Dephoure Noah N, Hwang Sunyoung S, O'Sullivan Ciara C, Dodgson Stacie E SE, Gygi Steven P SP, Amon Angelika A, Torres Eduardo M EMAneuploid Cell, Saccharomyces oviformis, Aneuploids, Yeast, Saccharomyces italicus., Aneuploid, number, baker's yeast, Saccharomyes cerevisiae, Saccharomyces uvarum var. melibiosus, presence, Cell, Saccaromyces cerevisiae, Aneuploid Cells, Sccharomyces cerevisiae, Aneuploidies, count in organism, Candida robusta, count, Saccharomyces capensis, Cells, yeast, proteomic analysis, quantitative, brewer's yeast, lager beer yeast, presence or absence in organismprojections, biochemical pathways, protein levels, scale tissue, Metabolic Process, Materials, lamellae, Processes, Neoplasms, peltate hair, number, Metabolic Concepts, Gene, Tumor, Metabolic Processes, process of organ, presence, NEOPL, protrusion, lamella, Endomycetale, Aneuploidies, Metabolism, Concepts, Metabolism Concept, Phenomenon, Sprains, Metabolism Phenomena, Aneuploid Cell, Genetic, Aneuploid, catabolism, plant peltate hair, Metabolic Concept, tumour, proteins, metabolic process resulting in cell growth, ridges, number of, inhibition of homeostatic process, Aneuploid Cells, MALIGNANT AND UNSPECIFIED (INCL CYSTS AND POLYPS), Budding Yeast, Endomycopses, papilla, has or lacks parts of type, negative regulation of homeostatic process, homeostasis, biotransformation, Strains, associated, laminae, Catabolism, Neoplastic Growth, Budding, Tumors, Budding Yeasts, Autoregulation, Saccharomycetale, incorporation, Aneuploids, data, anatomical protrusion, degradation, Process, anatomical process, metabolism resulting in cell growth, Sprain, lamina, flanges, extra or missing physical or functional parts, Cistrons, Cell, tumours, Concept, Metabolic Phenomena, mereological quality, polypeptide, Metabolism Concepts, count in organism, Experiment, count, shelf, Strain, Phenomena, Genetic Materials, scales, regulation of homeostatic process, metabolism, flange, Genetic Material, organ process, Metabolic Phenomenon, Yeasts, Yeast, mobilization, multicellular organism metabolic process, scale, biodegradation, Metabolic, shelves, projection, ridge, turnover, process, processes, single-organism metabolic process, activation of homeostatic process, positive regulation of homeostatic process., spine, Material, Endomycopsis, Cells, cardinality, processus, Cistron, quantitative, Strains and Sprains, Proteomes, NEOPLASMS BENIGN, Neoplasia, Endomycetales, presence or absence in organism, AnabolismAneuploid Cell, Saccharomyces oviformis, Aneuploids, Yeast, Saccharomyces italicus., Aneuploid, number, baker's yeast, Saccharomyes cerevisiae, Saccharomyces uvarum var. melibiosus, presence, Cell, Saccaromyces cerevisiae, Aneuploid Cells, Sccharomyces cerevisiae, Aneuploidies, count in organism, Candida robusta, count, Saccharomyces capensis, Cells, yeast, proteomic analysis, quantitative, brewer's yeast, lager beer yeast, presence or absence in organismprojections, biochemical pathways, protein levels, scale tissue, Metabolic Process, Materials, lamellae, Processes, Neoplasms, peltate hair, number, Metabolic Concepts, Gene, Tumor, Metabolic Processes, process of organ, presence, supernumerary, NEOPL, protrusion, lamella, Endomycetale, Aneuploidies, Metabolism, Concepts, Cell., Metabolism Concept, Phenomenon, Sprains, Metabolism Phenomena, Reactive, Aneuploid Cell, increased, Genetic, Aneuploid, catabolism, plant peltate hair, Metabolic Concept, tumour, proteins, metabolic process resulting in cell growth, ridges, number of, inhibition of homeostatic process, Oxygen Species, c-ros-1, Aneuploid Cells, Oxygen, MALIGNANT AND UNSPECIFIED (INCL CYSTS AND POLYPS), Budding Yeast, Endomycopses, papilla, has or lacks parts of type, negative regulation of homeostatic process, homeostasis, biotransformation, Strains, associated, laminae, Active Oxygen, Catabolism, Neoplastic Growth, Budding, Tumors, Budding Yeasts, Autoregulation, Saccharomycetale, incorporation, Aneuploids, Pro Oxidants, anatomical protrusion, degradation, Process, anatomical process, metabolism resulting in cell growth, Sprain, lamina, flanges, extra or missing physical or functional parts, Cistrons, Cell, tumours, results, Concept, Metabolic Phenomena, mereological quality, polypeptide, Metabolism Concepts, count in organism, count, positive regulation of homeostatic process, shelf, Strain, Phenomena, Genetic Materials, Active, scales, ROS, regulation of homeostatic process, metabolism, Oxygen Radicals, flange, Genetic Material, organ process, Metabolic Phenomenon, Yeasts, Yeast, mobilization, multicellular organism metabolic process, scale, biodegradation, Metabolic, increased number, shelves, projection, ridge, turnover, Fitness and Exercise, process, processes, present in greater numbers in organism, single-organism metabolic process, activation of homeostatic process, MCF3, spine, Material, Endomycopsis, Cells, cardinality, Pro-Oxidants, processus, Cistron, quantitative, Strains and Sprains, Proteomes, NEOPLASMS BENIGN, Neoplasia, Endomycetales, accessory, presence or absence in organism, Anabolism2806http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002303dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0project~1uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.008636644046884640.01.0falseQuantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.Data from ProteomeXchange, PXD ID: PXD001019. Experiment: Dis14, file: folder summary. Published as part of Elife. 2014 Jul 29:e03023 . From the Abstract: {{i}} Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis ... {{/i}}2014-08-18GPM3232000230325073701PXD001019PXD001019