GPMDB00185OtherEnglish JA, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mousedrcotter@rcsi.ie24194745http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002676http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002654http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002653http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002675http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002656http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002678http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002677http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002655http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002658http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002679http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002657http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002659http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002690http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002670http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002692http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002691http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002694http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002650http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002672http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002693http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002671http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002674http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002652http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002695http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002651http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002673http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002665http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002687http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002664http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002667http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002689http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002688http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002666http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002669http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002668http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002649http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002648http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002681http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002680http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002683http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002661http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002660http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002682http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002663http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002685http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002662http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002684Department of Psychiatry, Royal College of Surgeons in Ireland, ERC Beaumont Hospital Dublin, IrelandNot availableGPMDBOmega-3 fatty acid (n-3 FA) deficiency is an environmental risk factor for schizophrenia, yet characterization of the consequences of deficiency at the protein level in the brain is limited. We aimed to identify the protein pathways disrupted as a consequence of chronic n-3 deficiency in the hippocampus of mice. Fatty acid analysis of the hippocampus following chronic dietary deficiency revealed a 3-fold decrease (p < 0.001) in n-3 FA levels. Label free LC-MS/MS analysis identified and profiled 1008 proteins, of which 114 were observed to be differentially expressed between n-3 deficient and control groups (n = 8 per group). The cellular processes that were most implicated were neuritogenesis, endocytosis, and exocytosis, while specific protein pathways that were most significantly dysregulated were mitochondrial dysfunction and clathrin mediated endocytosis (CME). In order to characterize whether these processes and pathways are ones influenced by antipsychotic medication, we used LC-MS/MS to test the differential expression of these 114 proteins in the hippocampus of mice chronically treated with the antipsychotic agent haloperidol. We observed 23 of the 114 proteins to be differentially expressed, 17 of which were altered in the opposite direction to that observed following n-3 deficiency. Overall, our findings point to disturbed synaptic function, neuritogenesis, and mitochondrial function as a consequence of dietary deficiency in n-3 FA. This study greatly aids our understanding of the molecular mechanism by which n-3 deficiency impairs normal brain function, and provides clues as to how n-3 FA exert their therapeutic effect in early psychosis.Omega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus.English Jane A JA,Harauma Akiko A,Föcking Melanie M,Wynne Kieran K,Scaife Caitriona C,Cagney Gerard G,Moriguchi Toru T,Cotter David R DR,English Jane A JA, Harauma Akiko A, Föcking Melanie M, Wynne Kieran K, Scaife Caitriona C, Cagney Gerard G, Moriguchi Toru T, Cotter David R DRammon gyrus, Horn, 3.4.22.-, Cornu ammonis, Ammon, plasma membrane invagination, Laboratory, Ammon horn fields, formatio hippocampi, Proper, Mus domesticus, Proteins, ammon horn, mouse, Hippocampus Propers, Ammon Horn, mini-ICE, Hippocampal Formation, Endocytoses, Mitochondrial Protein, CASP-14, hippocampus proper, nonselective vesicle endocytosis, House Mouse, mice C57BL/6xCBA/CaJ hybrid, seahorse, Mus muscaris, Subiculums, endocytic import into cell, vesicle endocytosis, archipallium, Hippocampal, House, Mus, Formations, Schaffer Collaterals, Protein, Mini-ICE, Mus musculus domesticus, major hippocampus, Ammon's Horn, Mice, Cornu Ammonis, Subiculum, Hippocampus, Mus musculus, cornu ammonis, Mitochondrial, hippocampus proprius, Ammon's, Ammon's horn, Propers, Caspase-14 subunit p10, Hippocampus Proper., Swiss, hippocampus (Crosby), mice, Swiss Mouse, House Mice, Swiss Mice, CNS - Brain - Hippocampus (MMHCC), Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, hippocampus major, Ammons Horn, Laboratory Mice, domesticus, Schaffer, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], hippocampus, primal cortex, Ammons horn, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, house mouse, Laboratory Mouse, Hippocampal Formations, Formation, Collateralsthe brain, liquid chromatography tandem mass spectroscopy, OCT, acido graso, 3.4.22.-, Disorders, Entire brain, acidos grasos, determination, PLXN2, Ammon horn fields, Laboratory, Ass-1, Mus domesticus, ammon horn, Gene, mini-ICE, slow time, CASP-14, Encephalons, House Mouse, unspecified (disorder), LC-MS-MS, Schizophrenia NOS, unspecified, Risk Factors, Dementia Praecox, SCHIZO NOS-CHR/EXACERB, acides gras, Formations, House, Schaffer Collaterals, Other specified types of schizophrenia, AA408052, LC-MSMS, chronic state, Diets, Gene Products, Mus musculus domesticus, fold, unspecified state, synganglion, Unspecified schizophrenia, subchronic state, Cornu Ammonis, Mice, Hippocampus, Group, SCHIZOPHRENIA NEC-UNSPEC, Schizophrenia (disorder), LCMSMS, Unspecified schizophrenia (disorder), Brains, hippocampus (Crosby), Swiss, Schizophrenic disorders, 2810428A13Rik, Swiss Mice, proteins, Groups, Schizophrenic Disorder, suprasegmental levels of nervous system, hippocampus major, Ammons Horn, free, Schaffer, ASS, Control Group., Ammons horn, OC([*])=O, Disorder, SCHIZOPHRENIA NEC-REMISS, mKIAA0463, SCHIZO NOS-SUBCHR/EXACER, Collaterals, LC-MS2, ammon gyrus, Horn, Cornu ammonis, data, PlexA2, SCHIZO NEC-CHR/EXACERB, suprasegmental structures, Ammon, schizophrenia, formatio hippocampi, SCHIZO NEC-SUBCHR/EXACER, Proper, Proteins, mouse, LC-MS/MS, Hippocampus Propers, Ammon Horn, schizophrenia-1, Hippocampal Formation, hippocampus proper, Factor, Fettsaeuren, increased time, AA589422, encephalon, chronic, seahorse, Subiculums, polypeptide, Fettsaeure, LC/MS/MS, archipallium, AW457381, Hippocampal, increased period, Mus, Schizophrenic disorders (disorder), SCHIZOPHRENIA NEC-SUBCHR, Mini-ICE, chemical analysis, Protein, SCHIZOPHRENIA NEC-CHR, high time, Schizophrenias, major hippocampus, NOS, Ammon's Horn, prolonged period, subchronic state with acute exacerbation, CHO2R, Subiculum, Hippocampus Proper, cornu ammonis, Mus musculus, [X]Schizophrenia, hippocampus proprius, Ammon's, Ammon's horn, Factors, Schizophrenia, Propers, Risk, Caspase-14 subunit p10, Schizophrenic, mice, Dietary, Encephalon, Swiss Mouse, Control, CNS - Brain - Hippocampus (MMHCC), House Mice, Caspase-14 subunit p19, acide gras, Schizophrenia NOS (disorder), MICE, Laboratory Mice, domesticus, in remission, Protein Gene Products, Schizophrenic Disorders, Gene Proteins, hippocampus, fatty acids, primal cortex, liquid chromatography-tandem mass spectroscopy, SCHIZOPHRENIC DIS, liquid chromatography tandem mass spectrometry, chronic state with acute exacerbation, Mouse, assay, SCHIZOPHRENIA NOS-UNSPEC, Plxn2, Hippocampal Formations, Laboratory Mouse, Formationammon gyrus, Horn, 3.4.22.-, Cornu ammonis, Ammon, plasma membrane invagination, Laboratory, Ammon horn fields, formatio hippocampi, Proper, Mus domesticus, Proteins, ammon horn, mouse, Hippocampus Propers, Ammon Horn, mini-ICE, Hippocampal Formation, Endocytoses, Mitochondrial Protein, CASP-14, hippocampus proper, nonselective vesicle endocytosis, House Mouse, mice C57BL/6xCBA/CaJ hybrid, seahorse, Mus muscaris, Subiculums, endocytic import into cell, vesicle endocytosis, archipallium, Hippocampal, House, Mus, Formations, Schaffer Collaterals, Protein, Mini-ICE, Mus musculus domesticus, major hippocampus, Ammon's Horn, Mice, Cornu Ammonis, Subiculum, Hippocampus, Mus musculus, cornu ammonis, Mitochondrial, hippocampus proprius, Ammon's, Ammon's horn, Propers, Caspase-14 subunit p10, Hippocampus Proper., Swiss, hippocampus (Crosby), mice, Swiss Mouse, House Mice, Swiss Mice, CNS - Brain - Hippocampus (MMHCC), Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, hippocampus major, Ammons Horn, Laboratory Mice, domesticus, Schaffer, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], hippocampus, primal cortex, Ammons horn, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, house mouse, Laboratory Mouse, Hippocampal Formations, Formation, Collateralsliquid chromatography tandem mass spectroscopy, projections, neuroleptique, acido graso, Disorders, Entire brain, acidos grasos, determination, Ammon horn fields, Laboratory, Ass-1, Mus domesticus, CASP-14, nonselective vesicle endocytosis, House Mouse, acquired immune deficiency syndrome, dysfunctional, endocytic import into cell, Readability, unspecified, Risk Factors, Dementia Praecox, Formations, InChIKey=LNEPOXFFQSENCJ-UHFFFAOYAL, AA408052, chronic state, symptoms, fold, unspecified state, synganglion, 4-[4-(4-chlorophenyl)-4-hydroxypiperidin-1-yl]-1-(4-fluorophenyl)butan-1-one, Unspecified schizophrenia, Group, average, SCHIZOPHRENIA NEC-UNSPEC, LCMSMS, Unspecified schizophrenia (disorder), Acquired Immunodeficiency Syndromes, antipsychotic agents, N, Schizophrenic disorders, Swiss Mice, IMMUNODEFIC SYNDROME ACQUIRED, proteins, Groups, antipsychotics, suprasegmental levels of nervous system, Ammons Horn, free, Neuroleptikum, Schaffer, ASS, Ammons horn, papilla, haloperidolum, clathrin-dependent endocytosis, SCHIZO NOS-SUBCHR/EXACER, Collaterals, LC-MS2, ammon gyrus, ACQUIRED IMMUNO DEFIC SYNDROME, neuroleptic agents, screening, 26H, Acquired Immuno-Deficiency Syndrome, suprasegmental structures, Immunodeficiency Syndrome, Ammon, anatomical protrusion, formatio hippocampi, mouse, LC-MS/MS, lamina, flanges, Ammon Horn, antipsychotiques, hippocampus proper, neuroleptics, Fettsaeuren, Normalcy, AIDS - Acquired immunodeficiency syndrome, Immuno-Deficiency Syndromes, brain structure, seahorse, partial functionality, neuroleptiques, Acquired human immunodeficiency virus infection syndrome NOS, Subiculums, vesicle endocytosis, 4-[4-(4-chlorophenyl)-4-hydroxy-1-piperidyl]-1-(4-fluorophenyl)-butan-1-one, 4'-fluoro-4-(4-hydroxy-4-(4'-chlorophenyl)piperidino)butyrophenone, Hippocampal, AIDS, increased period, SCHIZOPHRENIA NEC-SUBCHR, Mini-ICE, shelf, precocious, Schizophrenias, major hippocampus, NOS, concluding remarks, Ammon's Horn, 4-(4-(para-chlorophenyl)-4-hydroxypiperidino)-4'-fluorobutyrophenone, Subiculum, 4-(4-(4-chlorophenyl)-4-hydroxy-1-piperidinyl)-1-(4-fluorophenyl)-, Mus musculus, [X]Schizophrenia, Ammon's, Acquired, Ammon's horn, Factors, acquired immunedeficiency syndrome, Risk, Caspase-14 subunit p10, mice, shelves, gamma-(4-(p-chlorophenyl)-4-hydroxpiperidino)-p-fluorbutyrophenone, early., Encephalon, Acquired immune deficiency syndrome (AIDS) (disorder), conclusion, Swiss Mouse, Control, signs, Caspase-14 subunit p19, lacks function of type, MICE, major tranquilizing agents, projection, ridge, low functionality, domesticus, summary, Schizophrenic Disorders, fatty acids, Health, primal cortex, Syndromes, spine, liquid chromatography-tandem mass spectroscopy, ACQUIRED IMMUNODEFICIENCY SYNDROME, chronic state with acute exacerbation, Mouse, Acquired immune deficiency syndrome (AIDS), the brain, Brain, 3.4.22.-, advanced, lamellae, clathrin-mediated endocytosis, ammon horn, Gene, impaired, Acquired immune deficiency syndrome, mini-ICE, slow time, Endocytoses, Encephalons, CME, process of organ, unspecified (disorder), LC-MS-MS, protrusion, lamella, Schizophrenia NOS, conclusions, SCHIZO NOS-CHR/EXACERB, acides gras, House, Schaffer Collaterals, Other specified types of schizophrenia, LC-MSMS, central nervous system, Diets, Gene Products, Mus musculus domesticus, Immuno-Deficiency Syndrome, subchronic state, Cornu Ammonis, Mice, Hippocampus, Normalities, OC1(CCN(CCCC(=O)c2ccc(F)cc2)CC1)c1ccc(Cl)cc1, study, Schizophrenia (disorder), Nervous System, Acquired immunodeficiency syndrome, AIDS (disorder), Brains, CNS, hippocampus (Crosby), Swiss, Direction, antipsychotic drugs, dysfunction, Schizophrenic Disorder, ridges, hippocampus major, antipsychotic, 1-(3-p-fluorobenzoylpropyl)-4-p-chlorophenyl-4-hydroxypiperidine, Acquired Immuno Deficiency Syndrome, OC([*])=O, grosser Tranquilizer, Acquired Immuno-Deficiency, C21H23ClFNO2, Disorder, Syndrome, ACQUIRED IMMUNODEFIC SYNDROME, Control Group, vesicle exocytosis, SCHIZOPHRENIA NEC-REMISS, laminae, IMMUNOL DEFIC SYNDROME ACQUIRED, nonselective vesicle exocytosis, Horn, clathrin coated pit-dependent endocytosis, ACQUIRED IMMUNE DEFIC SYNDROME, Cornu ammonis, SCHIZO NEC-CHR/EXACERB, 4'-fluoro-4-(4-(p-chlorophenyl)-4-hydroxypiperidinyl)butyrophenone, findings, Haldol, plasma membrane invagination, schizophrenia, SCHIZO NEC-SUBCHR/EXACER, Proper, anatomical process, Proteins, Hippocampus Propers, acquired Immune deficiency, schizophrenia-1, Hippocampal Formation, 1-2, Factor, function, acquired human immunodeficiency virus infection syndrome NOS (disorder), Acquired Immunodeficiency, increased time, encephalon, neuroleptic, chronic, Acquired immune defic. syndr., polypeptide, Fettsaeure, LC/MS/MS, archipallium, Mus, Schizophrenic disorders (disorder), 1-Butanone, chemical analysis, Protein, SCHIZOPHRENIA NEC-CHR, high time, InChI=1/C21H23ClFNO2/c22-18-7-5-17(6-8-18)21(26)11-14-24(15-12-21)13-1-2-20(25)16-3-9-19(23)10-4-16/h3-10, prolonged period, subchronic state with acute exacerbation, CHO2R, Immunologic Deficiency Syndrome, flange, Hippocampus Proper, organ process, cornu ammonis, hippocampus proprius, Schizophrenia, Propers, Schizophrenic, Normality, Dietary, Immunodeficiency due to human immunodeficiency virus infection, CNS - Brain - Hippocampus (MMHCC), House Mice, acide gras, Schizophrenia NOS (disorder), Understanding, 11-15H2, Laboratory Mice, in remission, Protein Gene Products, Gene Proteins, process, processes, major tranquilizers, hippocampus, having decreased function, Immunodeficiency Syndromes, Acquired Immuno-Deficiency Syndromes, SCHIZOPHRENIC DIS, liquid chromatography tandem mass spectrometry, processus, assay, SCHIZOPHRENIA NOS-UNSPEC, Hippocampal Formations, Laboratory Mouse, Formation1805http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320002655dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0055521283158544110.01.0falseOmega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus.Data from ProteomeXchange, PXD ID: PXD001277. File: 120329kw_JEnglish_JE12_2.mzml. Published as part of Front Genet. 2013 Oct 28;4:208 . From the Abstract: {{i}} Omega-3 fatty acid (n-3 FA) deficiency is an environmental risk factor for schizophrenia, yet characterization of the consequences of deficiency at the protein level in the brain is limited. We aimed to identify the protein pathways disrupted as a consequence of chronic n-3 deficiency in the hippocampus of mice. Fatty acid analysis of the hippocampus following chronic dietary deficiency revealed a 3-fold decrease (p < 0.001) in n-3 FA levels. Label free LC-MS/MS analysis identified and profiled 1008 proteins, of which 114 were observed to be differentially expressed between n-3 deficient and control groups (n = 8 per group) ... {{/i}}2014-09-02GPM3232000265524194745PXD001277PXD001277