{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":15,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Tape CJ, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Mus_musculus_viruses, Mouse"],"submitter_mail":["christopher.tape@icr.ac.uk"],"publication":["25233145"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003601","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003568","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003602","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003569","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003566","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003567","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003600","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003605","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003606","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003603","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003604","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003560","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003561","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003564","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003565","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003562","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003563","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003609","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003607","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003608","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003577","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003610","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003611","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003578","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003571","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003572","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003570","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003575","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003576","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003573","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003574","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003588","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003589","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003582","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003583","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003580","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003581","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003586","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003587","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003584","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003585","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003590","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003558","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003599","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003559","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003593","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003594","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003591","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003592","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003597","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003598","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003595","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003596"],"submitter_affiliation":["Cancer Research UK Manchester Institute"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Reproducible, comprehensive phosphopeptide enrichment is essential for studying phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid (45 min), reproducible (r2 ≥ 0.80) and high-fidelity (>90% purity) phosphopeptide purification in a 96-well format. Automated phosphopeptide enrichment demonstrates reproducible synthetic phosphopeptide recovery across 2 orders of magnitude, \"well-to-well\" quantitative reproducibility indistinguishable to internal SILAC standards, and robust \"plate-to-plate\" reproducibility across 5 days of independent enrichments. As a result, automated phosphopeptide enrichment enables statistical analysis of label-free phosphoproteomic samples in a high-throughput manner. This technique uses commercially available, off-the-shelf components and can be easily adopted by any laboratory interested in phosphoproteomic analysis. We provide a free downloadable automated phosphopeptide enrichment program to facilitate uniform interlaboratory collaboration and exchange of phosphoproteomic data sets."],"pubmed_title":["Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC."],"pubmed_authors":["Tape Christopher J CJ,Worboys Jonathan D JD,Sinclair John J,Gourlay Robert R,Vogt Janis J,McMahon Kelly M KM,Trost Matthias M,Lauffenburger Douglas A DA,Lamont Douglas J DJ,Jørgensen Claus C,","Tape Christopher J CJ, Worboys Jonathan D JD, Sinclair John J, Gourlay Robert R, Vogt Janis J, McMahon Kelly M KM, Trost Matthias M, Lauffenburger Douglas A DA, Lamont Douglas J DJ, Jørgensen Claus C"],"name_synonyms":["TSC-1., Magnetic, IMAC, MIP-4alpha, SCYA26, MIP-4a"],"description_synonyms":["KI-RAS, projections, PLXN5, JCAP, Slf, lamellae, Slpa, NS3, Kras2, Eseptin, Particle, Sphere, Somatomedin-C, Nl1, FPH2, PLEXIN-B1, process of organ, phosphorylation, SeP, protrusion, lamella, ras, CEH, Latex, Kras-2, NEPII, NL1, NL2, Sint1, SEH, C-K-RAS, AF17q25, KRAS2, KRAS1, SEP, p21B, study, K-RAS4B, K-RAS4A, uniform, somatomedin-C, SF, CACP, Kitl, ridges, Mast cell growth factor, sEP, MSF, Msf, Microsphere, IMAC, MIP-4a, porous, IGF1, papilla, Mechano growth factor, SELP, Soluble KIT ligand, Latex Particles, Sl, laminae, Latex Particle, IGF-I, constant, data, SeptD1, steel factor, Microbead, anatomical protrusion, Magnetic., Spheres, Ki-ras, K-RAS2B, K-RAS2A, hematopoietic growth factor KL, anatomical process, TSC-1, lamina, Phosphorylations, FNZ, somatomedin, flanges, K-ras, Mell1, FINC, c-Ki-ras, HAPO, LETS, sKITLG, CIG, SINT1, Latex Sphere, Microbeads, MMEL2, mechano growth factor, p21, Particles, shelf, PNUTL4, ED-B, AI929937, Igf-1, flange, Beads, organ process, NAPB, SHEP7, NS, SZP, FN, mast cell growth factor, shelves, Stem cell factor, Latex Spheres, Magnetic, MIP-4alpha, Latex Beads, STAT5, Bead, projection, ridge, process, processes, stem cell factor, KL-1, SCYA26, RASK2, spine, CFC2, MGF, c-Kit ligand, KITLG, processus, GFND2, GFND, Eph2, MSF1, Latex Bead, SCF, NEP2"],"pubmed_title_synonyms":["TSC-1., Magnetic, IMAC, MIP-4alpha, SCYA26, MIP-4a"],"pubmed_abstract_synonyms":["projections, constant, Microbead, criteria, anatomical protrusion, artificial sequence, determination, Spheres, Laboratory, lamellae, anatomical process, TSC-1, Particle, Sphere, lamina, number, Phosphorylations, flanges, artificial gene, synthetic DNA, guidelines, process of organ, presence, phosphorylation, protrusion, lamella, count in organism, Latex Sphere, Microbeads, count, Latex, Particles, chemical analysis, shelf, synthetic, synthetic genetic interaction (sensu inequality), flange, Beads, organ process, shelves, Latex Spheres, uniform, Magnetic, min, synthetic genetic interaction defined by inequality, MIP-4alpha, Latex Beads, ridges, Bead, projection, synthetic constructs, ridge, free, process, processes, Microsphere, IMAC, SYNTHETIC CONSTRUCT sequences, SCYA26, MIP-4a, spine, porous, data., papilla, processus, artificial, Latex Bead, assay, quantitative, Latex Particles, laminae, Latex Particle, statistical analysis, presence or absence in organism"],"view_count":["15"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320003560"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.0046267735965453425"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.","description":"Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18  . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid ..., reproducible ... and high-fidelity ... phosphopeptide purification in a 96-well format ... {{/i}}","dates":{"submission":"2014-09-22"},"accession":"GPM32320003560","cross_references":{"pubmed":["25233145"],"Pride":["PXD000892"],"pride":[],"Pride Archive":["PXD000892"]}}