GPMDB00275OtherLin Qi, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mouseshajh@njmu.edu.cn25293948Nanjing Medical University, China, et al.http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004514http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004515http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004512http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004513http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004518http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004519http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004516http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004538http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004517http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004539http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004525http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004548http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004523http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004545http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004546http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004524http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004549http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004540http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004543http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004521http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004522http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004544http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004541http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004520http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004542Not availableGPMDBSpermatogenesis is a complex process closely associated with the phosphorylation-orchestrated cell cycle. Elucidating the phosphorylation-based regulations should advance our understanding of the underlying molecular mechanisms. Here we present an integrative study of phosphorylation events in the testis. Large-scale phosphoproteome profiling in the adult mouse testis identified 17,829 phosphorylation sites in 3955 phosphoproteins. Although only approximately half of the phosphorylation sites enriched by IMAC were also captured by TiO2, both the phosphoprotein data sets identified by the two methods significantly enriched the functional annotation of spermatogenesis. Thus, the phosphoproteome profiled in this study is a highly useful snapshot of the phosphorylation events in spermatogenesis. To further understand phosphoregulation in the testis, the site-specific kinase-substrate relations were computationally predicted for reconstructing kinase-substrate phosphorylation networks. A core sub-kinase-substrate phosphorylation networks among the spermatogenesis-related proteins was retrieved and analyzed to explore the phosphoregulation during spermatogenesis. Moreover, network-based analyses demonstrated that a number of protein kinases such as MAPKs, CDK2, and CDC2 with statistically more site-specific kinase-substrate relations might have significantly higher activities and play an essential role in spermatogenesis, and the predictions were consistent with previous studies on the regulatory roles of these kinases. In particular, the analyses proposed that the activities of POLO-like kinases (PLKs) might be dramatically higher, while the prediction was experimentally validated by detecting and comparing the phosphorylation levels of pT210, an indicator of PLK1 activation, in testis and other tissues. Further experiments showed that the inhibition of POLO-like kinases decreases cell proliferation by inducing G2/M cell cycle arrest. Taken together, this systematic study provides a global landscape of phosphoregulation in the testis, and should prove to be of value in future studies of spermatogenesis.Systematic analysis of the phosphoproteome and kinase-substrate networks in the mouse testis.Qi Lin L,Liu Zexian Z,Wang Jing J,Cui Yiqiang Y,Guo Yueshuai Y,Zhou Tao T,Zhou Zuomin Z,Guo Xuejiang X,Xue Yu Y,Sha Jiahao J,Qi Lin L, Liu Zexian Z, Wang Jing J, Cui Yiqiang Y, Guo Yueshuai Y, Zhou Tao T, Zhou Zuomin Z, Guo Xuejiang X, Xue Yu Y, Sha Jiahao J3.4.22.-, determination, reproductive system of male organism gonad, Laboratory, testicle, Mus domesticus, gonada of male genitals, mouse, male organism reproductive system gonad, gonad of male organism reproductive system, mini-ICE, CASP-14, gonad of male genitals, House Mouse, Phosphotransferases, genitalia of male organism gonad, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, male genitalia gonada, male genitals gonad, House, Mus, gonad of genitalia of male organism, male reproductive system gonad, chemical analysis, Mini-ICE, gonad of male genitalia, Mus musculus domesticus, Kinase, testiculus, Mice, genitalia of male organism gonada, gonada of reproductive system of male organism, Mus musculus, Transphosphorylases, Caspase-14 subunit p10, gonada of male reproductive system, Kinases, Swiss, Testicle, mice, gonada of genitalia of male organism, gonada of male organism reproductive system, Swiss Mouse, male gonad, gonad of male organism genitalia, House Mice, Swiss Mice, Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, gonad of reproductive system of male organism, Laboratory Mice, domesticus, male reproductive system gonada, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], orchis, Testicles, male genitalia gonad, Entire testis., "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, assay, house mouse, male organism reproductive system gonada, testes, gonada of male genitalia, ATP Phosphotransferases, male organism genitalia gonada, male organism genitalia gonad, Laboratory Mouse, Testes, ATPOCT, big, 3.4.22.-, scale tissue, Spermiogenesis, Bhlha41, Procedures, mol, PLXN2, testicle, Laboratory, adult stage, peltate hair, Mus domesticus, male organism reproductive system gonad, bHLHa48, mini-ICE, CASP-14, SCXB, House Mouse, phosphorylation, SCXA, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, male genitalia gonada, large, House, male reproductive system gonad, Method, gonad of male genitalia, Studies, Mus musculus domesticus, testiculus, Mice, adult, study, cell, Testicle, Swiss, gonada of genitalia of male organism, gonada of male organism reproductive system, plant peltate hair, gonad of male organism genitalia, 2810428A13Rik, Swiss Mice, present in organism, procedures, generation of spermatozoa., "mouse" EXACT common_name [], gonad of reproductive system of male organism, "Mus muscaris" RELATED misnomer [], Study, IMAC, MIP-4a, Methodological Studies, orchis, great, male genitalia gonad, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], house mouse, testes, male organism genitalia gonada, mKIAA0463, data, PlexA2, reproductive system of male organism gonad, TSC-1, gonada of male genitals, gonad of male organism reproductive system, mouse, Phosphorylations, Spermatocytogenesis, Procedure, gonad of male genitals, AA589422, Cell, genitalia of male organism gonad, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Experiment, AW457381, male genitals gonad, Mus, gonad of genitalia of male organism, BB114693, Mini-ICE, techniques, scales, genitalia of male organism gonada, Adults, gonada of reproductive system of male organism, Entire testis, Mus musculus, adults, scale, Caspase-14 subunit p10, gonada of male reproductive system, mice, Swiss Mouse, expanded, male gonad, MIP-4alpha, House Mice, Caspase-14 subunit p19, Methodological, MICE, Methodological Study, male reproductive system gonada, Laboratory Mice, domesticus, phosphorylated protein, "house mouse" EXACT genbank_common_name [], SCYA26, enlarged, Testicles, Mouse, Prot/PhosRes+, male organism reproductive system gonada, gonada of male genitalia, male organism genitalia gonad, Plxn2, Laboratory Mouse, Testes, Scl, methodology3.4.22.-, determination, reproductive system of male organism gonad, Laboratory, testicle, Mus domesticus, gonada of male genitals, mouse, male organism reproductive system gonad, gonad of male organism reproductive system, mini-ICE, CASP-14, gonad of male genitals, House Mouse, Phosphotransferases, genitalia of male organism gonad, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, male genitalia gonada, male genitals gonad, House, Mus, gonad of genitalia of male organism, male reproductive system gonad, chemical analysis, Mini-ICE, gonad of male genitalia, Mus musculus domesticus, Kinase, testiculus, Mice, genitalia of male organism gonada, gonada of reproductive system of male organism, Mus musculus, Transphosphorylases, Caspase-14 subunit p10, gonada of male reproductive system, Kinases, Swiss, Testicle, mice, gonada of genitalia of male organism, gonada of male organism reproductive system, Swiss Mouse, male gonad, gonad of male organism genitalia, House Mice, Swiss Mice, Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, gonad of reproductive system of male organism, Laboratory Mice, domesticus, male reproductive system gonada, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], orchis, Testicles, male genitalia gonad, Entire testis., "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, assay, house mouse, male organism reproductive system gonada, testes, gonada of male genitalia, ATP Phosphotransferases, male organism genitalia gonada, male organism genitalia gonad, Laboratory Mouse, Testes, ATPprojections, scale tissue, testicle, Laboratory, adult stage, Mus domesticus, male organism reproductive system gonad, CASP-14, House Mouse, phosphorylation, Cell Growth in Number, Readability, Roles, Method, Cdc2a, gonad of male genitalia, Cell Number Growth, Concepts, Kinase, adult, Cell Division Cycles, Growth, Testicle, gonada of male organism reproductive system, plant peltate hair, Tissue, Swiss Mice, present in organism, proteins, procedures, number of, generation of spermatozoa., IMAC, Methodological Studies, papilla, Cell Cycles, Role Concepts, male genitalia gonad, CDC2, Cdc2, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], house mouse, associated, ATP Phosphotransferases, ATP, anatomical protrusion, reproductive system of male organism gonad, TSC-1, gonada of male genitals, lamina, mouse, flanges, extra or missing physical or functional parts, Procedure, gonad of male genitals, Division Cycles, mice C57BL/6xCBA/CaJ hybrid, predicted, Role Concept, Playthings and Play, Mini-ICE, shelf, Role, Plaything, Cellular, Adults, activation, gonada of reproductive system of male organism, Entire testis, Mus musculus, Transphosphorylases, adults, Caspase-14 subunit p10, Division Cycle, mice, shelves, cessation of cell cycle, Swiss Mouse, expanded, Toys, male gonad, p33(CDK2), Caspase-14 subunit p19, Methodological, MICE, projection, Methodological Study, ridge, domesticus, SCYA26, enlarged, spine, Proliferation, Testicles, Cell Number, Mouse, male organism reproductive system gonada, male organism genitalia gonad, Protein Kinase, CDKN2, big, 3.4.22.-, Spermiogenesis, Plays, Procedures, lamellae, peltate hair, nucleocapsid, number, Gene, mini-ICE, arrest of cell cycle progression, process of organ, protrusion, gonada of male organism genitalia, lamella, gonad of male reproductive system, reproductive system of male organism gonada, male genitalia gonada, large, House, male reproductive system gonad, termination of cell cycle, Cycle, Studies, Gene Products, Mus musculus domesticus, testiculus, Number Growth, Mice, Toy, Cycles, study, Playthings, CDC28A, Swiss, gonada of genitalia of male organism, Cell Division Cycle, cell-division cycle, gonad of male organism genitalia, P34CDC2, Cell Division, ridges, STPK13, "mouse" EXACT common_name [], gonad of reproductive system of male organism, ATP-protein phosphotransferase, "Mus muscaris" RELATED misnomer [], Study, cell proliferation, Puppets, MIP-4a, orchis, great, has or lacks parts of type, Play, testes, laminae, male organism genitalia gonada, Puppet, Multiplication, data, Cellular Proliferation, CRCS10, anatomical process, Proteins, gonad of male organism reproductive system, Phosphorylations, POLD, Spermatocytogenesis, Cell, genitalia of male organism gonad, Phosphotransferases, Mus muscaris, Concept, mereological quality, polypeptide, A630093N05Rik, generation of spermatozoa, male genitals gonad, 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methodology2705http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004513dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0083281924737816160.01.0falseSystematic analysis of the phosphoproteome and kinase-substrate networks in the mouse testis.Data from ProteomeXchange, PXD ID: PXD000767. Experiment: IMAC-1, file: mmtestis-5mg-SCX_ID9_6mm-IMACenriched-ql-0-2.mzXML. Published as part of Mol Cell Proteomics. 2014 Oct 7 . From the Abstract: {{i}} ... Here we present an integrative study of phosphorylation events in the testis. Large-scale phosphoproteome profiling in the adult mouse testis identified 17,829 phosphorylation sites in 3,955 phosphoproteins. Although only approximately half of the phosphorylation sites enriched by IMAC were also captured by TiO2, both the phosphoprotein datasets identified by the two methods significantly enriched the functional annotation of spermatogenesis ... {{/i}}2014-10-14GPM3232000451325293948PXD000767PXD000767