GPMDB00265OtherBileck A, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanchristopher.gerner@univie.ac.at25347463University of Vienna, Faculty of Chemistry, Department of Analytical Chemistryhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004833http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004831http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004832http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004837http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004838http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004835http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004836http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004851http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004830http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004850http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004839http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004844http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004822http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004845http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004842http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004820http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004843http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004821http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004848http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004826http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004849http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004824http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004846http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004847http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004825http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004840http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320004841CL:0000842 mononuclear cellGPMDBInflammation is a physiological process involved in many diseases. Monitoring proteins involved in regulatory effects may help to improve our understanding of inflammation. We have analyzed proteome alterations induced in peripheral blood mononuclear cells (PBMCs) upon inflammatory activation in great detail using high-resolution mass spectrometry. Moreover, the activated cells were treated with dexamethasone to investigate their response to this antiphlogistic drug. From a total of 6886 identified proteins, 469 proteins were significantly regulated upon inflammatory activation. Data are available via ProteomeXchange with identifiers PXD001415-23. Most of these proteins were counter-regulated by dexamethasone, with some exceptions concerning members of the interferon-induced protein family. To confirm some of these results, we performed targeted MRM analyses of selected peptides. The inflammation-induced upregulation of proteins such as IL-1β, IL-6, CXCL2, and GROα was confirmed, however, with strong quantitative interindividual differences. Furthermore, the inability of dexamethasone to downregulate inflammation-induced proteins such as PTX3 and TSG6 was clearly demonstrated. In conclusion, the relation of cell function as well as drug-induced modulation thereof was successfully mapped to proteomes, suggesting targeted analysis as a novel and powerful drug evaluation method. Although most consequences of dexamethasone were found to be compatible with the expected mode of action, some unexpected but significant observations may be related to adverse effects.<h4>Objective</h4>To determine the histopathologic features associated with ultrasonographic echogenicity of thyroid nodules.<h4>Materials and methods</h4>This study included 95 nodules of 95 patients (76 women, 19 men; mean age 47.5 ± 12.9 years) with homogeneous echogenicity in which core needle biopsy was performed during a one year period. The nodule echogenicity was categorized into 4 grades (hyperechogenicity, isoechogenicity, mild hypoechogenicity, and marked hypoechogenicity). The biopsy specimens were evaluated by a pathologist regarding the histopathologic features of fibrosis, lymphoid infiltration, microfollicular pattern, uniform follicular pattern, and hypercellularity in nodules. We evaluated the association of each histopathologic feature among 3 categories of nodule echogenicity by multinomial regression analysis.<h4>Results</h4>The nodule echogenicity was isoechoic in 28 (29.5%), mildly hypoechoic in 37 (38.9%), and markedly hypoechoic in 30 (31.6%), and there was no hyperechoic nodule. There was a trend of increasing frequency of fibrosis (> 30%) as nodule echogenicity decreased (isoechogenicity, 10.7%; mild hypoechogenicity, 32.4%; and marked hypoechogenicity, 80%; <i>p</i> < 0.001). The microfollicular pattern, uniform follicular pattern, and hypercellularity were frequently found in mildly hypoechoic nodules than in isoechoic nodules (<i>p</i> ≤ 0.018). The fibrosis (> 30%) and hypercellularity were independently associated with mild or marked hypoechogenicity as compared to isoechogenicity (fibrosis; <i>p</i> ≤ 0.004 and hypercellularity; <i>p</i> ≤ 0.036), and only fibrosis (> 30%) was independently associated with marked hypoechogenicity as compared to mild hypoechogenicity (<i>p</i> = 0.004).<h4>Conclusion</h4>The fibrosis (> 30%) and high cellularity are independently associated with mild or marked hypoechogenicity of nodules. The knowledge of the relationship of echogenicity and histopathology of thyroid nodules could improve management of patients with thyroid nodules.Comprehensive assessment of proteins regulated by dexamethasone reveals novel effects in primary human peripheral blood mononuclear cells.Ultrasonographic Echogenicity and Histopathologic Correlation of Thyroid Nodules in Core Needle Biopsy Specimens.Bileck Andrea A,Kreutz Dominique D,Muqaku Besnik B,Slany Astrid A,Gerner Christopher C,Bileck Andrea A, Kreutz Dominique D, Muqaku Besnik B, Slany Astrid A, Gerner Christopher CKim Ji-Hoon JH, Na Dong Gyu DG, Lee Hunkyung HOradexon, 19+, 15+, human being, 9-fluoro-11, whole blood, Blood, Proteins, dexametasona, InChI=1/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13, Dexpak, 2)21(15, Gene, Homo sapiense, Hexadecadrol, InChIKey=UREBDLICKHMUKA-CXSFZGCWBM, 12, polypeptide, 15-17, Homo spaiens, 17, 1-Dehydro-16alpha-methyl-9alpha-fluorohydrocortisone, 16+, Homo sapien, blood cells, Dexasone, Homo sapian, 20+, Homo sapians, Protein, Gene Products, Millicorten, C22H29FO5, Cell., Decaject, Decadron, Homo sapeins, 8, 9, [H][C@@]12C[C@@H](C)[C@](O)(C(=O)CO)[C@@]1(C)C[C@H](O)[C@@]1(F)[C@@]2([H])CCC2=CC(=O)C=C[C@]12C, dexamethasonum, 21-trihydroxy-16alpha-methylpregna-1, Decaject-L.A., Maxidex, 16alpha-Methyl-9alpha-fluoro-1-dehydrocortisol, 9-fluoro-11beta, 24, 28H, Hexadrol, 26, 17+, 16alpha)-, Homo sapients, Pregna-1, Peripheral Blood, 22+/m1/s1, 23)17(26)10-20(16, 21+, 28)18(27)11-24/h6-7, (11beta, 20-dione, Decaspray, Humo sapiens, Decameth, proteins, circulating cell, man, Homo sapines, 4-5, human, 4-diene-3, 3)22(12, Protein Gene Products, 1-3H3/t12-, Reticuloendothelial System, Gene Proteins, Homo spiens, "human" EXACT genbank_common_name [], circulating cells, 21-trihydroxy-16-methyl-, Homo sapience, Homo sampiens, Decaject L.A., 10-11H2, Methylfluorprednisolone, 9alpha-Fluoro-16alpha-methylprednisolone, Home sapiensOCT, big, IGF-I, data, PlexA2, steel factor, Inflammations, whole blood, 4Cytl1, PLXN2, PBMC cell, Slf, hematopoietic growth factor KL, Blood, Proteins, Somatomedin-C, somatomedin, Gene, FPH2, Spectrum Analyses, AA589422, Spectrum Analysis, Cell, sKITLG, Spectroscopy, polypeptide, Readability, large, AW457381, C17, blood cells, PBMC, mechano growth factor, Protein, Gene Products, Mass, Gm147, Analysis, Igf-1, activation, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, Mass Spectrum, SHEP7, PBM cell, inflammatory response, Peripheral Blood, mast cell growth factor, Analyses, Stem cell factor, somatomedin-C, expanded, Spectrometry, SF, Kitl, 2810428A13Rik, inflammation, proteins, circulating cell, Understanding, STAT5, Mast cell growth factor, Protein Gene Products, Reticuloendothelial System, Gene Proteins, stem cell factor, KL-1, circulating cells, 4930443F05Rik, enlarged, Inflammation, IGF1, MGF, great, c-Kit ligand, Mechano growth factor, Mass., KITLG, Cyt1, Soluble KIT ligand, SCF, Sl, Plxn2, Proteomes, mKIAA0463Oradexon, 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Protein Gene Products, 1-3H3/t12-, Reticuloendothelial System, Gene Proteins, Homo spiens, "human" EXACT genbank_common_name [], circulating cells, 21-trihydroxy-16-methyl-, Homo sapience, Homo sampiens, Decaject L.A., 10-11H2, Methylfluorprednisolone, 9alpha-Fluoro-16alpha-methylprednisolone, Home sapiensExtended Families, Networks, Oradexon, CDF, Inflammations, 9-fluoro-11, Extended, Kinship, single-organism process, determination, Blood, Il-6, 2)21(15, Extended Family, Evaluation Studies, B-cell stimulatory factor 2, InChIKey=UREBDLICKHMUKA-CXSFZGCWBM, 12, Drug Evaluation Study, Polypeptides, Readability, IL-1, 17, 1-Dehydro-16alpha-methyl-9alpha-fluorohydrocortisone, ak, Method, responsivity, IL-6, Interferon beta-2, AI607804, Life Cycle, Hybridoma growth factor, Analysis, 8, 9, Family Life Cycle, Mass Spectrum Analysis, 16alpha-Methyl-9alpha-fluoro-1-dehydrocortisol, 9-fluoro-11beta, 24, strong, 26, Drug Evaluation, inflammatory response, Kinship Network, Analyses, (11beta, Decaspray, Decameth, proteins, hybridoma growth factor, TSG-14, interleukin-6 receptor ligand, Methodological Studies, Inflammation, medicine, physiological process, Methylfluorprednisolone, Family Life Cycles, biological process unknown, 15+, dexametasona, Dexpak, single organism process, Procedure, HSF, Spectrum Analysis, results, Multiple Reaction Monitoring, Spectroscopy, Evaluation, BSF2, 15-17, 16+, blood cells, count, Diseases, Millicorten, IL1F2, [H][C@@]12C[C@@H](C)[C@](O)(C(=O)CO)[C@@]1(C)C[C@H](O)[C@@]1(F)[C@@]2([H])CCC2=CC(=O)C=C[C@]12C, Filiation, activation, 21-trihydroxy-16alpha-methylpregna-1, Decaject-L.A., 28H, Hexadrol, 17+, Drug Evaluation Studies, Reconstituted Family, 20-dione, expanded, Spectrometry, Methodological, Methodological Study, 4-5, 4-diene-3, 1-3H3/t12-, side effects., Life Cycles, IFNB2, Reconstituted, enlarged, 21-trihydroxy-16-methyl-, Decaject L.A., Physiological Processes, Interferon, Proteomes, big, 19+, Family Member, Procedures, Evaluation Study, B-cell hybridoma growth factor, InChI=1/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13, number, Gene, interferon beta-2, Network, Spectrum Analyses, interleukin-1 receptor ligand, Hexadecadrol, presence, large, method, HGF, IL1-BETA, resilient, Evaluations, tough, method used in an experiment, Gene Products, Mass, Studies, Decaject, Decadron, CTL differentiation factor, Mass Spectroscopy, reactivity, 16alpha)-, MRM, Research, Drug Evaluations, 23)17(26)10-20(16, 28)18(27)11-24/h6-7, inflammation, ASMD, Study, biological_process, drugs, circulating cells, great, IL6, 10-11H2, Kinship Networks, Family, data, whole blood, Family Research, drug, Proteins, Reconstituted Families, ASOD, Cell, Family Members, polypeptide, count in organism, Dexasone, Stepfamily, 20+, IFN-beta-2, Protein, chemical analysis, BSF-2, C22H29FO5, biological process, dexamethasonum, Mass Spectrum Analyses, Maxidex, Mass Spectrum, Pregna-1, Peripheral Blood, 22+/m1/s1, 21+, SRM, Interleukin HP-1, circulating 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archive~1varsite~00Resource Reanalysis0.00.00.0080197409006785930.01.0falseComprehensive Assessment of Proteins Regulated by Dexamethasone Reveals Novel Effects in Primary Human Peripheral Blood Mononuclear Cells.Data from ProteomeXchange, PXD ID: PXD001416. File: PBMC_1_con_cyt1-4.mgf. Published as part of J Proteome Res. 2014 Oct 27 . From the Abstract: {{i}} Monitoring proteins involved in regulatory effects may help to improve our understanding of inflammation. We have analyzed proteome alterations induced in peripheral blood mononuclear cells (PBMCs) upon inflammatory activation in great detail using high resolution mass spectrometry. {{/i}}2014-11-10GPM3232000483025347463PXD001416PXD001416