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of Biology, Institute of Molecular Systems Biology ETH Zurich, et al."],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Cells respond to environmental stimuli via specialized signaling pathways. Concurrent stimuli trigger multiple pathways that integrate information, predominantly via protein phosphorylation. Budding yeast responds to NaCl and pheromone via two mitogen-activated protein kinase cascades, the high osmolarity, and the mating pathways, respectively. To investigate signal integration between these pathways, we quantified the time-resolved phosphorylation site dynamics after pathway co-stimulation. Using shotgun mass spectrometry, we quantified 2,536 phosphopeptides across 36 conditions. Our data indicate that NaCl and pheromone affect phosphorylation events within both pathways, which thus affect each other at more levels than anticipated, allowing for information exchange and signal integration. We observed a pheromone-induced down-regulation of Hog1 phosphorylation due to Gpd1, Ste20, Ptp2, Pbs2, and Ptc1. Distinct Ste20 and Pbs2 phosphosites responded differently to the two stimuli, suggesting these proteins as key mediators of the information exchange. A set of logic models was then used to assess the role of measured phosphopeptides in the crosstalk. Our results show that the integration of the response to different stimuli requires complex interconnections between signaling pathways."],"pubmed_title":["Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast."],"pubmed_authors":["Vaga Stefania S,Bernardo-Faura Marti M,Cokelaer Thomas T,Maiolica Alessio A,Barnes Christopher A CA,Gillet Ludovic C LC,Hegemann Björn B,van Drogen Frank F,Sharifian Hoda H,Klipp Edda E,Peter Matthias M,Saez-Rodriguez Julio J,Aebersold Ruedi R,","Vaga Stefania S, Bernardo-Faura Marti M, Cokelaer Thomas T, Maiolica Alessio A, Barnes Christopher A CA, Gillet Ludovic C LC, Hegemann Björn B, van Drogen Frank F, Sharifian Hoda H, Klipp Edda E, Peter Matthias M, Saez-Rodriguez Julio J, Aebersold Ruedi R"],"name_synonyms":["Saccharomyces oviformis, Sccharomyces cerevisiae, Yeast, biological signaling, Saccharomyces italicus., Candida robusta, signalling process, Saccharomyces capensis, signaling process, yeast, baker's yeast, Saccharomyes cerevisiae, brewer's yeast, Saccharomyces uvarum var. melibiosus, lager beer yeast, Saccaromyces cerevisiae, signalling, single organism signaling"],"description_synonyms":["Budding Yeasts, high osmolarity, Saccharomycetale, data, biological signaling, rock salt, mol, GLM2, Effects, mating, Longterm Effect, Phosphorylations, Spectrum Analyses, Spectrum Analysis, Cell, phosphorylation, Long Term, Spectroscopy, protein amino acid phosphorylation, Endomycetale, period, MHAM, common salt, halite, Natriumchlorid, PTEN1, MAPK, CWS1, Long Term Effects, kinase-related transforming protein, Mass, table salt, TEP1, Analysis, NaCl, Effect, ClNa, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, Yeasts, Mass Spectrum, Yeast, cell fusion, 10q23del, Analyses, Longterm, MMAC1, Spectrometry, chlorure de sodium, Long-Term, natrii chloridum, signalling, Longterm Effects, cloruro sodico, DEC, salt, Budding Yeast, signalling process, Endomycopses, signaling process, Endomycopsis, Kochsalz, Mass., Long-Term Effect, BZS, Long-Term Effects, time, Endomycetales, Budding, single organism signaling, sodium chloride"],"pubmed_title_synonyms":["Saccharomyces oviformis, Sccharomyces cerevisiae, Yeast, biological signaling, Saccharomyces italicus., Candida robusta, signalling process, Saccharomyces capensis, signaling process, yeast, baker's yeast, Saccharomyes cerevisiae, brewer's yeast, Saccharomyces uvarum var. melibiosus, lager beer yeast, Saccaromyces cerevisiae, signalling, single organism signaling"],"pubmed_abstract_synonyms":["IPP2A2, biological signaling, Logics, Effects, PTCH11, mating, Gene, mess1, Spectrum Analyses, Downregulation, A230106A15Rik, phosphorylation, Long Term, PHAPII, 5730420M11Rik, protein amino acid phosphorylation, Endomycetale, period, halite, template-activating factor I, Roles, responsivity, kinase-related transforming protein, Mass, Gene Products, Concepts, Mood, Analysis, Gpd1, MST3B, Effect, ClNa, Mass Spectroscopy, Gdc-1, Mass Spectrum Analysis, reactivity, Gpd-1, Set, SET, cell fusion, AI747587, HEL-S-95, Analyses, Longterm, TAF-I, PTCH, Moods, Sets (Psychology), STK3, phosphatase 2A inhibitor I2PP2A, chlorure de sodium, proteins, Long-Term, mKIAA4010, cloruro sodico, salt, IGAAD, Budding Yeast, 2PP2A, Endomycopses, signaling process, Role Concepts, Kochsalz, MST, AI785303, Long-Term Effect, Ptc1, PTC1, Long-Term Effects, Budding, single organism signaling., single organism signaling, Budding Yeasts, high osmolarity, Saccharomycetale, data, rock salt, Ste20, G6pd1, Affects, Proteins, Longterm Effect, Phosphorylations, StF-IT-1, 1810013H02Rik, Spectrum Analysis, Cell, results, Down Regulation, Mst2, Concept, Spectroscopy, Mst3, MST3, polypeptide, common salt, Down-Regulation, Role Concept, Natriumchlorid, Receptor Down-Regulation, I-2PP2A, MAPK, Down-Regulation (Physiology), Long Term Effects, Protein, Ptch, I2PP2A, Role, table salt, NaCl, HPE7, Mass Spectrum Analyses, Ptc, PTC, Yeasts, 0610042I06Rik, Mass Spectrum, Yeast, HLA-DR-associated protein II, distinct, Gdc1, Spectrometry, NBCCS, inhibitor of granzyme A-activated DNase, BCNS, natrii chloridum, mes, signalling, Longterm Effects, Protein Gene Products, Gene Proteins, 2610030F17Rik, TAF-IBETA, signalling process, Endomycopsis, MST-3, Receptor, response, C76483, AA407739, STE20, time, Endomycetales, sodium chloride"],"view_count":["29"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320005651"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride 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