GPMDB00265OtherRinschen MM, et al.InstrumentNot AvailableNot availableRatmarkus.rinschen@uk-koeln.de25420462University of Cologne, CECADhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007108http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007109http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007049http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007106http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007107http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007100http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007089http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007101http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007102http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007103http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007052http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007053http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007075http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007097http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007054http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007076http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007098http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007055http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007099http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007110http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007077http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007092http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007093http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007050http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007094http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007051http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007090http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007091http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007056http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007078http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007079http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007058http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007059http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007088http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007081http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007082http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007083http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007084http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007080Not availableGPMDBGlomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of phosphoproteomic analyses of cow and rat glomeruli to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel). Moreover, cross-species comparisons revealed conserved phosphorylation of the slit diaphragm protein nephrin on an acidic cluster at the intracellular terminus and conserved podocin phosphorylation on the very carboxyl terminus of the protein. We studied a highly conserved podocin phosphorylation site in greater detail and show that phosphorylation regulates affinity of the interaction with nephrin and CD2AP. Taken together, these results suggest that species comparisons of phosphoproteomic data may reveal regulatory principles in glomerular biology. All MS data have been deposited in the ProteomeXchange with identifier PXD001005 (http://proteomecentral.proteomexchange.org/dataset/PXD001005).Comparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture.Rinschen Markus M MM,Pahmeyer Caroline C,Pisitkun Trairak T,Schnell Nicole N,Wu Xiongwu X,Maaß Martina M,Bartram Malte P MP,Lamkemeyer Tobias T,Schermer Bernhard B,Benzing Thomas T,Brinkkoetter Paul T PT,Rinschen Markus M MM, Pahmeyer Caroline C, Pisitkun Trairak T, Schnell Nicole N, Wu Xiongwu X, Maaß Martina M, Bartram Malte P MP, Lamkemeyer Tobias T, Schermer Bernhard B, Benzing Thomas T, Brinkkoetter Paul T PTPhosphorylations, assay, phosphorylation., podocin, determination, chemical analysisSignal Transduction Systems, Signal Transductions, para Tyrosine, biological signaling, AUTSX5, Receptor-Mediated, Gene, Signal Transduction System, archinephron, "Bos Tauurus" RELATED misspelling [], CCN3, QM, phosphorylation, Receptor Mediated Signal Transduction, Signal Transduction, rat, Rattus sp. strain Wistar, Glomerular, Rats, cattle, Mammals, "Rattus rattiscus" RELATED misnomer [], Gene Products, excretory canal, Norway rat, Rattus rattiscus, "Bos primigenius taurus" RELATED synonym [], brown rat, "Gunn rats" RELATED misnomer [], head kidney, NEPH1., reference sample, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 3-(p-Hydroxyphenyl)alanine, Bos bovis, Signal, Pathways, "brown rat" EXACT common_name [], proteins, present in organism, Signal Pathways, IGFBP9, "Rattus norvegicus8" RELATED misspelling [], Signal Transduction Pathway, "bovine" EXACT common_name [], IBP-9, Entire kidney, Bos Tauurus, signalling pathway, L Tyrosine, L10, Epithelial Cells, signaling process, Tyr, Podocyte, Signal Transduction Pathways, domestic cow, Transductions, species, Tyrosin, NOVh, Signal Pathway, Controlled, single organism signaling, reniculate kidney, Rattus norvegicus8, data, Controlling, Pathway, cow, Proteins, kidney, 2-Amino-3-(p-hydroxyphenyl)propionic acid, Phosphorylations, DXS648, Receptor-Mediated Signal Transductions, Glomerular Visceral, "domestic cattle" EXACT common_name [], para-Tyrosine, embryonic kidney, Tyrosine, polypeptide, signaling cascade, Experiment, Rattus norwegicus, IGFBP-9, tirosina, Bos primigenius taurus, Receptor-Mediated Signal Transduction, Systems, Protein, "rat" EXACT common_name [], "Rattus sp. strain Wistar" EXACT equivalent_name [], metanephros, NOV, domestic cattle, signalling cascade, regulator, PlexA1, Transduction, "domestic cow" EXACT common_name [], Plxn1, "rats" EXACT common_name [], System, L-isomer, Visceral Epithelial Cells, "cattle" EXACT genbank_common_name [], C9H11NO3, rats, mKIAA4053, signalling, L isomer, Protein Gene Products, pronephric nephron, "cow" EXACT common_name [], Gene Proteins, Kidneys, signaling pathway, signalling process, pronephron, C130088N23Rik, "Norway rat" EXACT genbank_common_name [], Glomerular Visceral Epithelial Cells, "Rattus norwegicus" RELATED misspelling [], "Bos bovis" RELATED synonym [], 2600013D04Rik, L-Tyrosine, DXS648E, bovine, Gunn ratsPhosphorylations, assay, phosphorylation., podocin, determination, chemical analysisSignal Transduction Systems, Signal Transductions, para Tyrosine, biological signaling, nucleocytoplasm, AI461847, AL024079, Receptor-Mediated, Gene, Signal Transduction System, archinephron, "Bos Tauurus" RELATED misspelling [], phosphorylation, Receptor Mediated Signal Transduction, Signal Transduction, rat, Rattus sp. strain Wistar, Glomerular, Rats, CMS, cattle, congenital, Mammals, "Rattus rattiscus" RELATED misnomer [], Finnish type (nephrin) protein, C78928, Gene Products, excretory canal, CNF protein, Pgi, Norway rat, Rattus rattiscus, Gpi-1, CNF, "Bos primigenius taurus" RELATED synonym [], brown rat, protoplasm, "Gunn rats" RELATED misnomer [], head kidney, reference sample, NEPH1, protoplast, Gpi-1r, Nlk, Gpi-1s, 2-amino-3-(4-hydroxyphenyl)propanoic acid, 3-(p-Hydroxyphenyl)alanine, Phi, Bos bovis, Signal, Gpi-1t, Pathways, "brown rat" EXACT common_name [], proteins, present in organism, Signal Pathways, "Rattus norvegicus8" RELATED misspelling [], Signal Transduction Pathway, "bovine" EXACT common_name [], Entire kidney, nephrosis 1 homolog, Bos Tauurus, signalling pathway, podocin, L Tyrosine, Epithelial Cells, nephrin, signaling process, Tyr, NPHN, Podocyte, Signal Transduction Pathways, domestic cow, Transductions, species, Tyrosin, Signal Pathway, Controlled, single organism signaling, reniculate kidney, Rattus norvegicus8, Data Set., Gpi, NPHS1 protein, Controlling, data, Pathway, cow, Proteins, kidney, 2-Amino-3-(p-hydroxyphenyl)propionic acid, mouse, Phosphorylations, Receptor-Mediated Signal Transductions, Glomerular Visceral, "domestic cattle" EXACT common_name [], para-Tyrosine, Nphs1 protein, MF, Amf, results, embryonic kidney, Tyrosine, polypeptide, METS-1, signaling cascade, Rattus norwegicus, mOC-X, tirosina, Bos primigenius taurus, Receptor-Mediated Signal Transduction, Systems, Protein, "rat" EXACT common_name [], "Rattus sp. strain Wistar" EXACT equivalent_name [], metanephros, domestic cattle, NK|GPI, nephrin (human) protein, Mets1, internal to cell, signalling cascade, regulator, Transduction, "domestic cow" EXACT common_name [], NK, Org, ORG, "rats" EXACT common_name [], System, L-isomer, Visceral Epithelial Cells, "cattle" EXACT genbank_common_name [], Gpi1-r, Gpi1-s, C9H11NO3, Gpi1-t, rats, human, nephrosis 1, signalling, L isomer, Protein Gene Products, pronephric nephron, "cow" EXACT common_name [], Gene Proteins, Kidneys, signaling pathway, signalling process, pronephron, "Norway rat" EXACT genbank_common_name [], Glomerular Visceral Epithelial Cells, "Rattus norwegicus" RELATED misspelling [], "Bos bovis" RELATED synonym [], L-Tyrosine, bovine, Bglap-rs1, Gpi1s, Gunn rats2605http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007078dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0080197409006785930.01.0falseComparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture.Data from ProteomeXchange, PXD ID: PXD001005. Experiment: 130104MaRiS_TpD, file: 0089-130104MaRiS04TpD.mzml. Published as part of Proteomics. 2014 Nov 25 . From the Abstract: {{i}} Glomerular biology is dependent on tightly controlled signal transduction networks that control phosphorylation of signaling proteins such as cytoskeletal regulators or slit diaphragm proteins of kidney podocytes. Cross-species comparison of phosphorylation events is a powerful mean to functionally prioritize and identify physiologically meaningful phosphorylation sites. Here, we present the result of phosphoproteomic analyses of cow and rat glomeruli to allow cross-species comparisons. We discovered several phosphorylation sites with potentially high biological relevance, e.g. tyrosine phosphorylation of the cytoskeletal regulator synaptopodin and the slit diaphragm protein neph-1 (Kirrel) ... {{/i}}2015-01-21GPM3232000707825420462PXD001005PXD001005