GPMDB00525OtherGuo M, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mousem.trost@dundee.ac.uk25504905http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007637http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007638http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007639http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007643http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007642MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Science, University of DundeeCL:0000235 macrophageGPMDBMacrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicate that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. Moreover, bone marrow derived macrophages phagosomes mature considerably faster by fusion with endosomes and the lysosome which we validated using fluorogenic phagocytic assays. We provide a valuable resource for researcher in the field and recommend careful use of the RAW 264.7 cell line when studying phagosome functions. All MS data have been deposited in the ProteomeXchange with identifier PXD001293 (http://proteomecentral.proteomexchange.org/dataset/PXD001293).High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.Guo Manman M,Härtlova Anetta A,Dill Brian D BD,Prescott Alan R AR,Gierliński Marek M,Trost Matthias M,Guo Manman M, Härtlova Anetta A, Dill Brian D BD, Prescott Alan R AR, Gierliński Marek M, Trost Matthias MYellow Marrow, Phagolysosomes, head kidney, kidney marrow, determination, Phagosome, Monocyte Derived Macrophages, medullary bone, medulla of bone, Macrophages, number, Phagolysosome, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, Monocyte Derived, Bone marrow part, presence, Red Marrow, Bone Marrow-Derived, Bone Marrow-Derived Macrophages., count in organism, Monocyte-Derived, anterior kidney, count, medulla ossium, RAW 264.7, medulla ossea, Marrow, chemical analysis, Monocyte-Derived Macrophage, Red, assay, Yellow, quantitative, Proteomes, Bone, Monocyte-Derived Macrophages, presence or absence in organism, Macrophagemacrophagocyte, big, Gene., data, kidney marrow, GLM2, Monocyte Derived Macrophages, nonspecific immune response, Proteins, number, Cell Lines, innate immunity, Gene, cell_line, Monocyte Derived, future organ, extra or missing physical or functional parts, monocyte-derived macrophage, bone marrow-derived macrophage, Cell, mereological quality, polypeptide, MHAM, organ field, large, Monocyte-Derived, anterior kidney, medulla ossium, PTEN1, CWS1, medulla ossea, Marrow, Protein, Monocyte-Derived Macrophage, Line, Gene Products, field, TEP1, Yellow, Phagocytoses, Monocyte-Derived Macrophages, Macrophage, Lines, Yellow Marrow, Phagolysosomes, head kidney, 10q23del, Phagosome, Macrophages, medullary bone, medulla of bone, MMAC1, developmental field, expanded, Phagolysosome, cell line cell, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, proteins, number of, Bone marrow part, cell line, Red Marrow, Protein Gene Products, Bone Marrow-Derived, Gene Proteins, DEC, enlarged, RAW 264.7, great, has or lacks parts of type, cardinality, Bone Marrow-Derived Macrophages, Red, histiocyte, BZS, BoneYellow Marrow, Phagolysosomes, head kidney, kidney marrow, determination, Phagosome, Monocyte Derived Macrophages, medullary bone, medulla of bone, Macrophages, number, Phagolysosome, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, Monocyte Derived, Bone marrow part, presence, Red Marrow, Bone Marrow-Derived, Bone Marrow-Derived Macrophages., count in organism, Monocyte-Derived, anterior kidney, count, medulla ossium, RAW 264.7, medulla ossea, Marrow, chemical analysis, Monocyte-Derived Macrophage, Red, assay, Yellow, quantitative, Proteomes, Bone, Monocyte-Derived Macrophages, presence or absence in organism, Macrophagebig, kidney marrow, Lysosome, Monocyte Derived Macrophages, AI461847, nonspecific immune response, number, Gene, Sialic acid-binding Ig-like lectin 1, organ field, large, medulla ossea, Marrow, Monocyte-Derived Macrophage, Line, Gene Products, Pgi, field, Yellow, Gpi-1, phagosome, CD206 antigen, SER, Phagolysosomes, head kidney, Siglec-1, Phagosome, Receptosome, Gpi-1r, Nlk, Cd169, Gpi-1s, developmental field, Phi, Gpi-1t, Phagolysosome, cell line cell, Bone Marrow-Derived Macrophage, proteins, number of, Bone marrow part, cell line, Red Marrow, Bone Marrow-Derived, great, has or lacks parts of type, Red, Sn, CD169, macrophagocyte, Data Set., Gpi, data, use, mannosyl-fucosyl receptor, Proteins, Cell Lines, innate immunity, cell_line, Monocyte Derived, future organ, extra or missing physical or functional parts, MF, monocyte-derived macrophage, Amf, bone marrow-derived macrophage, Cell, Endosome, mereological quality, polypeptide, Monocyte-Derived, anterior kidney, mOC-X, medulla ossium, Protein, NK|GPI, Receptosomes, Phagocytoses, NK, Monocyte-Derived Macrophages, Macrophage, Lines, mannose receptors, Yellow Marrow, Org, ORG, Macrophages, medullary bone, medulla of bone, expanded, Gpi1-r, Bone Marrow Derived Macrophages, Gpi1-s, Gpi1-t, Sheep erythrocyte receptor, Protein Gene Products, Gene Proteins, mannose-fucose receptor, mannose, enlarged, RAW 264.7, cardinality, Bone Marrow-Derived Macrophages, receptor, histiocyte, Bone, Bglap-rs1, Gpi1s5205http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320007638dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0160394818013571870.01.0falseHigh-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.Data from ProteomeXchange, PXD ID: PXD001293. File: BMDM_2.mzml. Published as part of Proteomics. 2014 Dec 12 . From the Abstract: {{i}} Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicate that there are significant differences for a large number of proteins ... {{/i}}2015-02-10GPM3232000763825504905PXD001293PXD001293