GPMDB00141OtherWinget JM, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human, Micavibrio_aeruginosavorus_arl_13jwinget@systemsbiology.orgNot availableInstitute for Systems 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availableGPMDBsingle-organism biosynthetic process, skin, multicellular organismal biosynthetic process., formation, anabolism, plural), dermal tissue (exact), outer epithelial layer, biosynthesis, hypodermis, adult epidermis, epidermis, synthesis, Entire epidermis, multiseriate epidermis (related), vertebrate epidermis, 表皮 (Japanese, exact), portion of epidermal tissue (exact), epidermises (exact, epidermides (exact, epidermis (Spanish, epidermal layer (exact), epidermis (sensu Metazoa), outer epidermal layer, hypodermprojections, perfluoroethylene, 2-tetrafluoroethylene, AI461847, Effects, PH 3., lamellae, Entire dermis, dermal tissue (exact), TFE, BOUND WATER, Trifluoroethyl Alcohol, oxidane, process of organ, InChIKey=XLYOFNOQVPJJNP-UHFFFAOYAF, Long Term, agua, epidermis, CYCLE, HOH, protrusion, Entire epidermis, period, C2F4, lamella, multiseriate epidermis (related), SDS, Polypeptides, 1-(5-O-phosphono-D-ribofuranosyl)-1H-imidazol-5-amine, Ethanol, Electrophoreses, 表皮 (Japanese, exact), Ccne, AIR, SEF2-1, 1, E2.2, 2, Pgi, ITF2, Tetrafluoraethylen, Gpi-1, corium, Effect, Separated, C8H14N3O7P, Corium, Divorced, cutis, [H]O[H], skin, Longterm, ITF-2, Gpi-1r, Nlk, Gpi-1s, Phi, Tcf-4, 5'-Phosphoribosyl-5-aminoimidazole, Gpi-1t, ITF-2b, 4733401P19Rik, Divorces, outer epithelial layer, ridges, eau, Long-Term, SEF2, 5-Amino-1-(5-phospho-D-ribosyl)imidazole, portion of epidermal tissue (exact), SEF-2, papilla, epidermises (exact, dihydridooxygen, tetrafluoroethene, Long-Term Effect, epidermis (sensu Metazoa), dihydrogen oxide, ASP-I2, laminae, F2C=CF2, Long-Term Effects, Gpi, NEWBORN (0-27 DAYS), Neonatal, data, 2-Trifluoroethanol, anatomical protrusion, Alcohol, 2-trifluoro-, plural), anatomical process, 1-(5'-Phosphoribosyl)-5-aminoimidazole, aqua, lamina, Longterm Effect, AI836084, flanges, InChI=1/H2O/h1H2, MF, hypodermis, Amf, ME2, adult epidermis, H2O, Experiment, mOC-X, Long Term Effects, epidermides (exact, tetrafluoroethylene, shelf, Trifluoroethyl, epidermis (Spanish, epidermal layer (exact), NK|GPI, 1-(5'-phosphoribosyl)-5-aminoimidazole, Perfluoraethylen, flange, NK, Hydrogen Oxide, organ process, perfluoroethene, Separation, Org, ORG, 1-(5-Phospho-D-ribosyl)-5-aminoimidazole, Tetrafluoraethen, shelves, Separations, hydrogen hydroxide, E2-2, Gpi1-r, SWDS, Gpi1-s, Shwachman-Bodian-Diamond syndrome, Shwachman syndrome, Keratinocyte, CGI-97, Gpi1-t, projection, ridge, acqua, Longterm Effects, Aminoimidazole ribotide, process, processes, vertebrate epidermis, spine, Pancreatic insufficiency and bone marrow dysfunction, vertebrate dermis, 5730422P05Rik, processus, Newborn, Wasser, outer epidermal layer, [OH2], bHLHb19, hypoderm, time, Bglap-rs1, Gpi1s, MITF-2B, MITF-2A140http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM323200079271dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~1lineage-covid19~0metagenomics~0pfam~0varsite~00.00.00.004318322023442320.001.0falseReconstituted Epidermis timecourse of barrier formationData from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00363 PASS00363]]. Experiment: P2-D3, file: P2-D3-F6.mzml. From PeptideAtlas: {{i}} De-cellularized dermis was seeded with neonatal keratinocytes and cultured submerged in media for two days. At this point the cultures were raised to the air/water interface to stimulate differentiation. At the indicated time points, epidermis was removed with forceps. Epidermis was extracted in 50% Trifluoroethanol (TFE), 0.1% SDS, 100 mM Ammonium Bicarbonate (AMBIC). Samples were heated at 60 deg C for 30 minutes, then sonicated at 75 W for 2 minutes process time using a 50% duty cycle. Extracts were cleared by centrifugation. separation: Samples from each timepoint were randomly assigned to 4 pools. Pools of 250 ug peptides were separated with Offgel Electrophoresis, pH 3-10 into 12 fractions. {{/i}}2015-03-02GPM32320007927