{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":35,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Kasvandik S, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Cow"],"submitter_mail":["andres.salumets@ccrmb.ee"],"publication":["25603787"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014070","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014071","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014073","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014074","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014075","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014064","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014065","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014076","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014066","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014077","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014078","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014067","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014068","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014079","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014069"],"submitter_affiliation":["University of Tartu, Estonia"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm surface. These protein constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the bovine sperm cell apical plasma membrane (PM) proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm's surface. We used a cell surface biotin-labeling in combination with differential centrifugation to enrich sperm surface proteins. Using nano-LC MS/MS, 338 proteins were confidently identified in the PM-enriched proteome. Functional categories of sperm-egg interaction, protein turnover, metabolism as well as molecular transport, spermatogenesis, and signal transduction were represented by proteins with high quantitative signal in our study. A highly significant degree of enrichment was found for transmembrane and PM-targeted proteins. Among them, we also report proteins previously not described on bovine sperm (CPQ, CD58, CKLF, CPVL, GLB1L3, and LPCAT2B) of which CPQ and CPVL cell surface localization was further validated. A descriptive overview of the bovine sperm PM integral and peripheral proteins is provided to complement future studies on animal reproduction and its relation to sperm cell surface. All MS data have been deposited in the ProteomeXchange with identifier PXD001096 (http://proteomecentral.proteomexchange.org/dataset/PXD001096)."],"pubmed_title":["Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment."],"pubmed_authors":["Kasvandik Sergo S,Sillaste Gerly G,Velthut-Meikas Agne A,Mikelsaar Aavo-Valdur AV,Hallap Triin T,Padrik Peeter P,Tenson Tanel T,Jaakma Ülle Ü,Kõks Sulev S,Salumets Andres A,","Kasvandik Sergo S, Sillaste Gerly G, Velthut-Meikas Agne A, Mikelsaar Aavo-Valdur AV, Hallap Triin T, Padrik Peeter P, Tenson Tanel T, Jaakma Ülle Ü, Kõks Sulev S, Salumets Andres A"],"name_synonyms":["\"cow\" EXACT common_name [], Bos Tauurus, cattle, cow, Bos primigenius taurus, \"Bos bovis\" RELATED synonym [], Bos bovis, domestic cattle, \"cattle\" EXACT genbank_common_name [], domestic cow, \"domestic cattle\" EXACT common_name [], \"Bos Tauurus\" RELATED misspelling [], bovine, Biotinylations., \"domestic cow\" EXACT common_name [], \"Bos primigenius taurus\" RELATED synonym [], \"bovine\" EXACT common_name []"],"description_synonyms":["Conceptions, Viral Marker, Biological Markers, Deacura, Polyspermic Fertilization, Surrogate Endpoints, Clinical Markers, Laboratory, Clinical Marker, Biotine, Biochemical, metazoans, Medebiotin, Endpoint, Gene, Sialic acid-binding Ig-like lectin 1, \"Bos Tauurus\" RELATED misspelling [], Vitamin H, Serum, composed of, animals, Surrogate End Points, Delayed Fertilizations, Surrogate Markers, surface, Laboratory Markers, cattle, Biotin, Biological, 4beta, Gene Products, smooth ER, mature sperm cell, Animal, 6aalpha))-, Gelfert, Polyspermic, \"Bos primigenius taurus\" RELATED synonym [], integumentum commune, Animalia, SER, sperm cell, proportion, Biotin Hermes, Clinical, Siglec-1, reference sample, cell, N, Biological Marker, Bos bovis, composition, proteins, present in organism, labeling, \"bovine\" EXACT common_name [], zoosperm, inner endospore membrane, Bos Tauurus, plasma membrane lipid bilayer, Immunologic Markers, Immune, Markers, Hermes, Delayed Fertilization, sperm, plasmalemma, Viral Markers, n, SPERM, domestic cow, Immunologic Marker, CD169, Biologic, Controlled, ratio, ejaculate, Medobiotin, data, Controlling, Viral, Polyspermic Fertilizations, external covering of organism, Surrogate Endpoint, cow, Rombellin, Serum Markers, Proteins, End Point, Roche, Biotin Gelfert, organism surface, Biochemical Markers, \"domestic cattle\" EXACT common_name [], compositionality, Biologic Marker, Cell, spermatozoon, Immune Marker, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, polypeptide, cellular membrane, juxtamembrane, male gamete, Conception, Stickstoff, Marker, Surrogate End Point, Bos primigenius taurus, Metazoa, Protein, Proteomes., Biotin Ratiopharm, domestic cattle, bacterial inner membrane, \"domestic cow\" EXACT common_name [], Biodermatin, Biologic Markers, semen, Biotin-Ratiopharm, 1H-Thieno(3, Serum Marker, 10^[-9], Biotine Roche, cell membrane, Gabunat, End Points, spermatozoan, body surface, Surrogate, content, Endpoints, proportionality, hexahydro-2-oxo-, \"cattle\" EXACT genbank_common_name [], rate, Immunologic, azote, metazoan-, Laboratory Marker, Sheep erythrocyte receptor, spermatozoid, Surrogate Marker, nitrogeno, \"cow\" EXACT common_name [], Protein Gene Products, multicellular animals, seminal fluid, Gene Proteins, Delayed, Fertilization, nitrogen, Breedings, integumentary system, Biochemical Marker, syngamy, Fertilizations, structure, quotient, \"Bos bovis\" RELATED synonym [], spermatazoon, 7N, cytoplasmic membrane, bovine, dermal system, Proteomes, Biokur, Immune Markers"],"pubmed_title_synonyms":["\"cow\" EXACT common_name [], Bos Tauurus, cattle, cow, Bos primigenius taurus, \"Bos bovis\" RELATED synonym [], Bos bovis, domestic cattle, \"cattle\" EXACT genbank_common_name [], domestic cow, \"domestic cattle\" EXACT common_name [], \"Bos Tauurus\" RELATED misspelling [], bovine, Biotinylations., \"domestic cow\" EXACT common_name [], \"Bos primigenius taurus\" RELATED synonym [], \"bovine\" EXACT common_name []"],"pubmed_abstract_synonyms":["biochemical pathways, Conceptions, egg, Signal Transductions, Viral Marker, Biological Markers, Metabolic Process, Polyspermic Fertilization, Surrogate Endpoints, degree (angle), Laboratory, Metabolic Concepts, Biochemical, metazoans, Endpoint, Human Reproductive Indexes, \"Bos Tauurus\" RELATED misspelling [], Serum, composed of, cell associated, Delayed Fertilizations, Receptor Mediated Signal Transduction, Cklf, solute:solute exchange, Laboratory Markers, Tier, Biotin, Biological, 1700001K04Rik, Concepts, establishment and maintenance of substrate location, Metabolism Concept, 1700063K20Rik, Gpi-1, Phenomenon, animal, Reproductive Periods, integumentum commune, IKLF, Animalia, sperm cell, Human Reproductive Index, mature oocyte, reference sample, catabolism, Nlk, N, Bos bovis, Pathways, composition, proteins, present in organism, metabolic process resulting in cell growth, Bteb2, Bos Tauurus, Immune, Markers, Hermes, fully spanning plasma membrane, BTEB2, Viral Markers, n, reproductive physiological process, Periods, biotransformation, domestic cow, Signal Transduction Pathways, Transductions, Catabolism, AW494632, ratio, ejaculate, Data Set., incorporation, Gpi, Medobiotin, establishment and maintenance of substance location, Pathway, Viral, Polyspermic Fertilizations, external covering of organism, Surrogate Endpoint, Indices, Rombellin, Process, Index, ovum, animalia, metabolism resulting in cell growth, integral to membrane, organism surface, Biochemical Markers, AI449770, Biologic Marker, 4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha, Reproductive Index, signaling cascade, single organism localization, organism, 1700041N15Rik, male gamete, count, mOC-X, Stickstoff, single-organism transport, Marker, Human Reproductive Indices, Biotin Ratiopharm, NK|GPI, Transduction, semen, 1H-Thieno(3, Org, ORG, Biotine Roche, whole organism, End Points, content, System, proportionality, \"cattle\" EXACT genbank_common_name [], Gpi1-r, rate, Gpi1-s, Immunologic, metazoan-, Laboratory Marker, Gpi1-t, spermatozoid, turnover, \"cow\" EXACT common_name [], Pgcp, seminal fluid, Delayed, Fertilization, nitrogen, signaling pathway, Breedings, integumentary system, Biochemical Marker, syngamy, Aytl1b, Koerper, small molecule transport, 7N, bovine, KKLF, dermal system, cell bound, Proteomes, Biokur, Bglap-rs1, establishment and maintenance of cellular component location, Gpi1s, Lpcat2bp, Signal Transduction Systems, Spermiogenesis, Deacura, multi-cellular organism, Clinical Markers, AI461847, Processes, Clinical Marker, Biotine, Receptor-Mediated, number, Medebiotin, Gene, Signal Transduction System, Vitamin H, Metabolic Processes, animals, presence, Surrogate End Points, Human, Surrogate Markers, surface, Signal Transduction, cattle, Metabolism, UCK-1, 4beta, Gene Products, HVLP, establishment and maintenance of position, mature sperm cell, Pgi, Animal, 1810013I09Rik, 6aalpha))-, Gelfert, Metabolism Phenomena, Polyspermic, \"Bos primigenius taurus\" RELATED synonym [], CKLF5, CKLF4, study, CKLF3, proportion, Biotin Hermes, Clinical, Cklfsf2a, Gpi-1r, Gpi-1s, Biological Marker, Phi, Signal, Gpi-1t, Metabolic Concept, labeling, metazoa, Signal Pathways, Signal Transduction Pathway, Reproductive Indices, \"bovine\" EXACT common_name [], Cklfsf2-1b, zoosperm, signalling pathway, lal-1, Immunologic Markers, Delayed Fertilization, sperm, SPERM, LAL, Immunologic Marker, Signal Pathway, Human Reproductive, Biologic, Controlled, Reproductive, Controlling, data, 4921521K07Rik, cow, degradation, body, Cklf1, Serum Markers, Proteins, Reproductive Period, End Point, Receptor-Mediated Signal Transductions, ARR19, Roche, Spermatocytogenesis, Biotin Gelfert, whole body, \"domestic cattle\" EXACT common_name [], compositionality, MF, Amf, RGD1310837, spermatozoon, Immune Marker, Concept, Metabolic Phenomena, polypeptide, Metabolism Concepts, count in organism, generation of spermatozoa, Conception, Period, Surrogate End Point, Bos primigenius taurus, Receptor-Mediated Signal Transduction, Metazoa, Protein, Systems, Phenomena, domestic cattle, establishment and maintenance of localization, signalling cascade, metabolism, \"domestic cow\" EXACT common_name [], NK, Biodermatin, Metabolic Phenomenon, Biologic Markers, Biotin-Ratiopharm, 4930520J07Rik, mobilization, multicellular organism metabolic process, Serum Marker, 10^[-9], transmembrane, Gabunat, spermatozoan, biodegradation, Metabolic, body surface, Surrogate, Endpoints, hexahydro-2-oxo-, azote, arc degree, single-organism localization, Surrogate Marker, nitrogeno, Protein Gene Products, multicellular animals, Gene Proteins, C32, single-organism metabolic process, localisation, Fertilizations, Indexes, structure, quotient, \"Bos bovis\" RELATED synonym [], spermatazoon, quantitative, AV048136, CKLF, Immune Markers, Anabolism, presence or absence in organism"],"view_count":["35"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014064"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.0107958050586058"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment.","description":"Data from ProteomeXchange, PXD ID: PXD001096. File: 130508_01_Orbi1_SK_SER_G150_T158_BOSS_2xenrich_repl1_try.mzml. Published as part of Proteomics. 2015 Jan 20  . From the Abstract: {{i}} A significant proportion of mammalian fertilization is mediated through the proteomic composition of the sperm surface. These protein constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the bovine sperm cell apical plasma membrane proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm\"s surface. We used a cell-surface biotin-labelling in combination with differential centrifugation to enrich sperm surface proteins. Using nano-LC MS/MS, 338 proteins were confidently identified in the plasma membrane enriched proteome ... {{/i}}","dates":{"submission":"2015-03-21"},"accession":"GPM32320014064","cross_references":{"pubmed":["25603787"],"Pride":["PXD001096"],"pride":[],"Pride Archive":["PXD001096"]}}