GPMDB001735OtherBroncel M, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humane.tate@imperial.ac.uk25807930Department of Chemistry, Imperial College Londonhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014414Not availableGPMDBNovel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development.Broncel Malgorzata M,Serwa Remigiusz A RA,Ciepla Paulina P,Krause Eberhard E,Dallman Margaret J MJ,Magee Anthony I AI,Tate Edward W EW,Broncel Malgorzata M, Serwa Remigiusz A RA, Ciepla Paulina P, Krause Eberhard E, Dallman Margaret J MJ, Magee Anthony I AI, Tate Edward W EWHomo sapients, human being, wide/broad, protein modification, determination, single-organism developmental process., protein amino acid lipidation, number, Humo sapiens, broad, Homo sapiense, man, Homo sapines, presence, human, Cell, lipid:protein modification, development, count in organism, wide, Homo spaiens, Homo spiens, "human" EXACT genbank_common_name [], Homo sapien, count, Homo sapience, Homo sapian, Homo sampiens, Homo sapians, chemical analysis, assay, quantitative, Vertebrate, Homo sapeins, Home sapiens, Proteomes, presence or absence in organismEph-like tyrosine kinase 1, scale tissue, Postnidation Embryo, Procedures, Pre-implantation Embryo Development, determination, Slf, peltate hair, Somatomedin-C, number, Post-implantation, Gene, ETK1, FPH2, Spectrum Analyses, Development, Pre implantation Embryo Development, presence, Embryonic Programming, Endogl1, Endogl2, Prenidation Embryo Development, Polypeptides, method, Embryo Development, HEK4, Method, method used in an experiment, Embryonic Developments, Gene Products, Mass, Studies, PDZK6, Analysis, Animal, Human embryo kinase, TYRO4, Mass Spectroscopy, Tyrosine-protein kinase TYRO4, MAR, Mass Spectrum Analysis, Postnidation, ENGLA, ENGLB, Analyses, Identification, Englb, Engla, plant peltate hair, EK4, Engl, HeLa, somatomedin-C, Postnidation Embryo Development, SF, Kitl, proteins, Pre-implantation, Mast cell growth factor, PDZD6, Study, 2.7.10.1, Methodological Studies, IGF1, ENGL, Mechano growth factor, ENDOGL2, ENDOGL1, RCB0007, Soluble KIT ligand, Sl, ETK, IGF-I, data, steel factor, ENGL-a, ENGL-b, IRF-1, Preimplantation Embryo Development., hematopoietic growth factor KL, Proteins, Post implantation Embryo Development, somatomedin, Post-implantation Embryo Development, Preimplantation, Procedure, Spectrum Analysis, sKITLG, lipid:protein modification, Spectroscopy, polypeptide, count in organism, count, ENGL-B, mechano growth factor, Protein, chemical analysis, EPH-like kinase 4, Vertebrate, scales, Igf-1, Mass Spectrum Analyses, Postimplantation Embryo Development, Embryonic, Mass Spectrum, Embryo, SHEP7, mast cell growth factor, Lipids, scale, INT, protein amino acid lipidation, Stem cell factor, Spectrometry, Embryogenesis, Identifications (Psychology), hEK4, Methodological, STAT5, AW557704, Methodological Study, Protein Gene Products, plan specification, Gene Proteins, stem cell factor, KL-1, MGF, c-Kit ligand, HEK, KITLG, assay, quantitative, SCF, Tyrosine-protein kinase receptor ETK1, Proteomes, Postimplantation, Embryonic Programmings, presence or absence in organismHomo sapients, human being, wide/broad, protein modification, determination, single-organism developmental process., protein amino acid lipidation, number, Humo sapiens, broad, Homo sapiense, man, Homo sapines, presence, human, Cell, lipid:protein modification, development, count in organism, wide, Homo spaiens, Homo spiens, "human" EXACT genbank_common_name [], Homo sapien, count, Homo sapience, Homo sapian, Homo sampiens, Homo sapians, chemical analysis, assay, quantitative, Vertebrate, Homo sapeins, Home sapiens, Proteomes, presence or absence in organismscale tissue, Postnidation Embryo, Procedures, Pre-implantation Embryo Development, determination, Preimplantation Embryo Development., peltate hair, Proteins, number, Post implantation Embryo Development, Post-implantation Embryo Development, Post-implantation, Preimplantation, Gene, Spectrum Analyses, Development, Procedure, Pre implantation Embryo Development, Spectrum Analysis, presence, lipid:protein modification, Embryonic Programming, Spectroscopy, polypeptide, Prenidation Embryo Development, Polypeptides, method, count in organism, Embryo Development, count, Method, method used in an experiment, Protein, chemical analysis, Embryonic Developments, Gene Products, Mass, Studies, Analysis, Vertebrate, Animal, scales, Mass Spectrum Analyses, Postimplantation Embryo Development, Mass Spectroscopy, Mass Spectrum Analysis, Embryonic, Mass Spectrum, Postnidation, Embryo, Lipids, scale, Analyses, Identification, plant peltate hair, protein amino acid lipidation, Spectrometry, Postnidation Embryo Development, Embryogenesis, Identifications (Psychology), proteins, Methodological, Pre-implantation, Methodological Study, Protein Gene Products, plan specification, Study, Gene Proteins, Methodological Studies, assay, quantitative, Proteomes, Postimplantation, Embryonic Programmings, presence or absence in organism17035http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014414dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbekb~9pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0uniprot~21omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0052436767427513881.00.0falseMultifunctional Reagents for Quantitative Proteome-Wide Analysis of Protein Modification in Human Cells and Dynamic Profiling of Protein Lipidation During Vertebrate Development.Data from ProteomeXchange, PXD ID: PXD001863. File: HEK_HeLa_MCF_ PEAKS7.mgf. Published as part of Angew Chem Int Ed Engl. 2015 Mar 25 . From the Abstract: {{i}} Novel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.{{/i}}2015-04-01GPM3232001441425807930PXD001863PXD001863