GPMDB00205OtherBracht T, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanbarbara.sitek@rub.de25807371Ruhr-Universitaet Bochum Medizinisches Proteom-Center AG Clinical Proteomicshttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014456http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014457http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014458http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014459http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014450http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014451http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014452http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014453http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014454http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014455http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014467http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014445http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014468http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014446http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014447http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014469http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014448http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014460http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014461http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014462http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014463http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014464http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014465http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014443http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014444http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014466Not availableGPMDBHepatic fibrosis and cirrhosis are major health problems worldwide. Until now, highly invasive biopsy remains the diagnostic gold standard despite many disadvantages. To develop noninvasive diagnostic assays for the assessment of liver fibrosis, it is urgently necessary to identify molecules that are robustly expressed in association with the disease. We analyzed biopsied tissue samples from 95 patients with HBV/HCV-associated hepatic fibrosis using three different quantification methods. We performed a label-free proteomics discovery study to identify novel disease-associated proteins using a subset of the cohort (n = 27). Subsequently, gene expression data from all available clinical samples were analyzed (n = 77). Finally, we performed a targeted proteomics approach, multiple reaction monitoring (MRM), to verify the disease-associated expression in samples independent from the discovery approach (n = 68). We identified fibulin-5 (FBLN5) as a novel protein expressed in relation to hepatic fibrosis. Furthermore, we confirmed the altered expression of microfibril-associated glycoprotein 4 (MFAP4), lumican (LUM), and collagen alpha-1(XIV) chain (COL14A1) in association to hepatic fibrosis. To our knowledge, no tissue-based quantitative proteomics study for hepatic fibrosis has been performed using a cohort of comparable size. By this means, we add substantial evidence for the disease-related expression of the proteins examined in this study.Analysis of disease-associated protein expression using quantitative proteomics—fibulin-5 is expressed in association with hepatic fibrosis.Bracht Thilo T,Schweinsberg Vincent V,Trippler Martin M,Kohl Michael M,Ahrens Maike M,Padden Juliet J,Naboulsi Wael W,Barkovits Katalin K,Megger Dominik A DA,Eisenacher Martin M,Borchers Christoph H CH,Schlaak Jörg F JF,Hoffmann Andreas-Claudius AC,Weber Frank F,Baba Hideo A HA,Meyer Helmut E HE,Sitek Barbara B,Bracht Thilo T, Schweinsberg Vincent V, Trippler Martin M, Kohl Michael M, Ahrens Maike M, Padden Juliet J, Naboulsi Wael W, Barkovits Katalin K, Megger Dominik A DA, Eisenacher Martin M, Borchers Christoph H CH, Schlaak Jörg F JF, Hoffmann Andreas-Claudius AC, Weber Frank F, Baba Hideo A HA, Meyer Helmut E HE, Sitek Barbara Bpolypeptide, count in organism, Fbln1 protein, human disease, fibulin 1 protein, count, determination, chemical analysis, C elegans, Diseases, BM-90 protein, FBLN1 protein, number, mouse, Homo sapiens disease, assay, proteins, quantitative, associated, fbl-1 protein, presence, human, Associations., presence or absence in organismGene., data, human hepatitis C virus, Liver, Procedures, IRF-1, Proteins, post-transfusion hepatitis non A non B virus, Cirrhosis of liver NOS (disorder), Procedure, Client, Cirrhosis of liver, Hepatic Cirrhoses, polypeptide, Cirrhosis of liver without mention of alcohol, Method, Associations, liver cirrhosis, Protein, Diseases, Studies, Gene Products, Cirrhosis, tissue portion, NOS, Fibroses, simple tissue, Cirrhoses, techniques, Fibrosis, HCV, MAR, Cirrhosis of liver NOS, portion of tissue, human hepatitis C virus HCV, study, human disease, Liver Fibrosis, Tissue, Liver Fibroses, proteins, Methodological, procedures, Methodological Study, free, cirrhosis, Protein Gene Products, Study, Gene Proteins, hepatitis C virus HCV, Liver Cirrhoses, Methodological Studies, Patient, CL - Cirrhosis of liver, Clients, Hepatic, Homo sapiens disease, Hepatic cirrhosis, Cirrhosis of liver (disorder), associated, Proteomes, methodology, Hepatitis Bpolypeptide, count in organism, Fbln1 protein, human disease, fibulin 1 protein, count, determination, chemical analysis, C elegans, Diseases, BM-90 protein, FBLN1 protein, number, mouse, Homo sapiens disease, assay, proteins, quantitative, associated, fbl-1 protein, presence, human, Associations., presence or absence in organismCollagens, AU023367, Procedures, C elegans, post-transfusion hepatitis non A non B virus, ARMD3, microfibrillar associated glycoprotein, number, alpha-Collagen, Gene, MFAP2 protein, presence, Xt, Cirrhosis of liver, Hepatic Cirrhoses, FIBL-5, Protein Gm1141, Cirrhosis of liver without mention of alcohol, Microfibril, Method, Dermodress, Associations, Collastat, EVEC, CIRRH, Studies, Gene Products, Fibroses, Cirrhoses, Zyderm, microfibril-associated protein-1, Normalities, average, ADHD, portion of tissue, human hepatitis C virus HCV, study, Fbln1 protein, microfibril-associated glycoprotein-2, human disease, MAGP2 protein, Gene Expressions, Biopsies, MRM, DANCE, Microfibril Collagen Hemostat, Liver Fibrosis, Tissue, UP50, Avitene, study., ADCL2, 1110007F23Rik, proteins, procedures, fbl-1 protein, Expressions, free, alpha Collagen, Study, ch, hepatitis C virus HCV, Liver Cirrhoses, fibulin 1 protein, Methodological Studies, Clients, Collagen Fleece, BM-90 protein, Hepatic, Homo sapiens disease, Expression, Collagen, associated, Collagenfleece, human hepatitis C virus, data, ARCL1A, Liver, Proteins, Novel protein, mouse, elastic fiber microfibrillar protein, microfibrillar-associated protein 2, Cirrhosis of liver NOS (disorder), Normalcy, Lumican Proteoglycan, Procedure, Client, SLRR2D, Multiple Reaction Monitoring, add, A55, polypeptide, AAAP-40, count in organism, count, GLI3-190, aortic aneurysm-associated antigenic protein-40, Bos taurus, microfibril associated glycoprotein 3, liver cirrhosis, Protein, Diseases, FBLN1 protein, Collagen Hemostat, Cirrhosis, tissue portion, NOS, simple tissue, techniques, Fibrosis, HCV, MAGP-3 protein, Bph, Pangen, Cirrhosis of liver NOS, Epistemology, Ldc, LDC, UND, Normality, GLI3FL, AI854843, SRM, Liver Fibroses, Avicon, Methodological, MFAP1 protein, Methodological Study, Pdn, human, cirrhosis, Protein Gene Products, Gene Proteins, Health, AW108078, Patient, CL - Cirrhosis of liver, microfibril-associated glycoprotein, Collagen Felt, Hepatic cirrhosis, Attention Deficit Hyperactivity Disorder, quantitative, Cirrhosis of liver (disorder), MAGP-2, MAGP-3, MAGP-1, methodology, 5730412L22Rik, Hepatitis B, Mfap2 protein, presence or absence in organism2005http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320014466dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0061690314620604570.01.0falseAnalysis of disease-associated protein expression using quantitative proteomics - Fibulin-5 is expressed in association with hepatic fibrosis.Data from ProteomeXchange, PXD ID: PXD001474. File: OEI6412.mzml. Published as part of J Proteome Res. 2015 Mar 25 . From the Abstract: {{i}} In order to develop non-invasive diagnostic assays for the assessment of liver fibrosis it is urgently necessary to identify molecules which are robustly expressed in association with the disease. We analyzed biopsied tissue samples from 95 patients with HBV/HCV-associated hepatic fibrosis using three different quantification methods. We performed a label-free proteomics discovery study to identify novel disease-associated proteins using a subset of the cohort (n = 27). {{/i}}2015-04-04GPM3232001446625807371PXD001474PXD001474