{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":32,"searchCount":7},"additional":{"omics_type":["Other"],"submitter":["Papadopoulos P, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Homo_sapiens_viruses, Human"],"submitter_mail":["petros.papadopoulos@med.kuleuven.be"],"publication":["25870109"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015001","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015002","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015004"],"submitter_affiliation":["Department of Cell Biology, Erasmus MC, Rotterdam, et al."],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["The ordered assembly of a functional preinitiation complex (PIC), composed of general transcription factors (GTFs), is a prerequisite for the transcription of protein-coding genes by RNA polymerase II. TFIID, comprised of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is the GTF that is thought to recognize the promoter sequences allowing site-specific PIC assembly. Transcriptional cofactors, such as SAGA, are also necessary for tightly regulated transcription initiation. The contribution of the two TAF10-containing complexes (TFIID, SAGA) to erythropoiesis remains elusive. By ablating TAF10 specifically in erythroid cells in vivo, we observed a differentiation block accompanied by deregulated GATA1 target genes, including Gata1 itself, suggesting functional cross talk between GATA1 and TAF10. Additionally, we analyzed by mass spectrometry the composition of TFIID and SAGA complexes in mouse and human cells and found that their global integrity is maintained, with minor changes, during erythroid cell differentiation and development. In agreement with our functional data, we show that TAF10 interacts directly with GATA1 and that TAF10 is enriched on the GATA1 locus in human fetal erythroid cells. Thus, our findings demonstrate a cross talk between canonical TFIID and SAGA complexes and cell-specific transcription activators during development and differentiation."],"pubmed_title":["TAF10 Interacts with the GATA1 Transcription Factor and Controls Mouse Erythropoiesis."],"pubmed_authors":["Papadopoulos Petros P,Gutiérrez Laura L,Demmers Jeroen J,Scheer Elisabeth E,Pourfarzad Farzin F,Papageorgiou Dimitris N DN,Karkoulia Elena E,Strouboulis John J,van de Werken Harmen J G HJ,van der Linden Reinier R,Vandenberghe Peter P,Dekkers Dick H W DH,Philipsen Sjaak S,Grosveld Frank F,Tora Làszlò L,","Papadopoulos Petros P, Gutiérrez Laura L, Demmers Jeroen J, Scheer Elisabeth E, Pourfarzad Farzin F, Papageorgiou Dimitris N DN, Karkoulia Elena E, Strouboulis John J, van de Werken Harmen J G HJ, van der Linden Reinier R, Vandenberghe Peter P, Dekkers Dick H W DH, Philipsen Sjaak S, Grosveld Frank F, Tora Làszlò L"],"name_synonyms":["Globin Transcription Factor 1, Transcription Factor GATA1, 3.4.22.-, TAFII30, Laboratory, Mus domesticus, RBC differentiation., mouse, mini-ICE, CASP-14, Nuclear Factor Erythroid 1, Transcription Factor, erythropoiesis, House Mouse, NFE-1, Erythroid Transcription Factor 1, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, AU041226, NF E1 Erythroid Specific Transcription Factor, House, Mus, 30kDa, Mini-ICE, erythrocyte cell differentiation, NF-E1 Erythroid-Specific Transcription Factor, TAF2H, Mus musculus domesticus, TAF2A, Factor GATA1, Eryf1 Erythroid-Specific Transcription Factor, Mice, Mus musculus, Transcription, Transcription Factor NFE-1, red blood cell differentiation, Caspase-14 subunit p10, Eryf1 Erythroid Specific Transcription Factor, Swiss, Erythropoieses, GATA1, mice, Swiss Mouse, House Mice, Swiss Mice, Caspase-14 subunit p19, \"mouse\" EXACT common_name [], MICE, Laboratory Mice, domesticus, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], Taf2h, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Nuclear Factor-Erythroid 1, Mouse, house mouse, Laboratory Mouse"],"description_synonyms":["APR, CPD photolyase activity, TAFII30, Materials, PKDYS, PhrB photolyase activity, Laboratory, Mus domesticus, Somatomedin-C, pre-integration complex, CASP-14, CSMF, Homo sapiense, pigmented epithelium, composed of, House Mouse, General Transcription Factors, AU041226, Homo sapien, 30kDa, Homo sapians, Analysis, DA transporter, outer pigmented layer of retina, epithelium, Gata1, Mass Spectrum Analysis, Homo sapients, GATA-1, Analyses, GATA1, NOXA, somatomedin-C, pigmented retina, SF, Kitl, composition, Swiss Mice, proteins, Mast cell growth factor, DNA cyclobutane dipyrimidine photolyase activity, XLTT, NFE1, DNA-dependent, pigment epithelium of retina, NOR-1, DNA Dependent RNA Polymerase II, Solute carrier family 6 member 3, Homo sapience, Erythroid Cell, IGF1, Mechano growth factor, pigmented retina epithelium, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Soluble KIT ligand, house mouse, associated, GATA-binding factor 1, Sl, Home sapiens, Gata-1, IGF-I, PRE, steel factor, CHN, DNA-dependent transcription, deoxyribonucleic cyclobutane dipyrimidine photolyase activity, tri-(n-butyl )-phosphate, mouse, somatomedin, pigmented retinal epithelium, Spectrum Analysis, sKITLG, mice C57BL/6xCBA/CaJ hybrid, Spectroscopy, XLTDA, batch, retinal pigment, NF-E1, Homo sapian, mechano growth factor, Mini-ICE, dipyrimidine photolyase (photosensitive), Genetic Materials, XLANP, Igf-1, retinal pigment layer, Genetic Material, Gf-1, Mus musculus, SHEP7, mast cell growth factor, Caspase-14 subunit p10, RNA Pol II, retinal pigmented epithelium, mice, content, Stem cell factor, Swiss Mouse, Spectrometry, initiation, tri-n-butylphosphate, Eryf1, Caspase-14 subunit p19, STAT5, MICE, Homo sapines, human, RBC differentiation, domesticus, GF-1, stem cell factor, phr A photolyase activity, KL-1, DNA-photoreactivating enzyme, Material, Cells, KITLG, Cistron, Mouse, IGFBP3R, phosphoric acid tributyl ester, 3.4.22.-, transcription, GTF2D, ERYF1, human being, mol, Slf, single-organism developmental process., APOER, Gene, FPH2, mini-ICE, Spectrum Analyses, photoreactivating enzyme activity, NF-E1 DNA-binding protein, deoxyribodipyrimidine photolyase activity, stratum pigmentosum retinae, Homo spaiens, CD91, House, Mass, TAF2H, Mus musculus domesticus, eryf1, GF1, stratum pigmentosa retinae, transcription regulator activity, Gtf2d, TAF2A, Mice, Mass Spectroscopy, HDL4, deoxyribocyclobutadipyrimidine pyrimidine-lyase activity, Genetic, AI573420, cell, Erythropoieses, Swiss, ligand, \"mouse\" EXACT common_name [], man, \"Mus muscaris\" RELATED misnomer [], Taf2h, SCA17, A2MR, PIC, Homo sampiens, GTF2D1, LRP1A, DAT, serum total protein, data, cellular transcription, NOR1, DAT1, Nor1, LRP, hematopoietic growth factor KL, transcription preinitiation complex, preinitiation complex, compositionality, Cistrons, erythropoiesis, Cell, Mus muscaris, polypeptide, development, TBP, stratum pigmentosum (retina), Experiment, Mus, erythrocyte cell differentiation, deoxyribonucleic photolyase activity, TGFBR5, TFIID, Erythroid, Homo sapeins, Dat1, Mass Spectrum Analyses, General Transcription Initiation Complex Proteins, Mass Spectrum, RPE, red blood cell differentiation, tri-n-butyl phosphate, tributylphosphate, MINOR, photolyase activity, DNA-templated transcriptional preinitiation complex, Humo sapiens, House Mice, retinal pigment epithelium, p. pigmentosa retinae, Laboratory Mice, \"house mouse\" EXACT genbank_common_name [], Homo spiens, \"human\" EXACT genbank_common_name [], MGF, c-Kit ligand, structure, transcriptional pre-initiation complex, RNA Polymerase B, TEC, deoxyribonucleate pyrimidine dimer lyase (photosensitive), DNA-Dependent RNA Polymerase II, SCF, Laboratory Mouse, Minor"],"pubmed_title_synonyms":["Globin Transcription Factor 1, Transcription Factor GATA1, 3.4.22.-, TAFII30, Laboratory, Mus domesticus, RBC differentiation., mouse, mini-ICE, CASP-14, Nuclear Factor Erythroid 1, Transcription Factor, erythropoiesis, House Mouse, NFE-1, Erythroid Transcription Factor 1, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, AU041226, NF E1 Erythroid Specific Transcription Factor, House, Mus, 30kDa, Mini-ICE, erythrocyte cell differentiation, NF-E1 Erythroid-Specific Transcription Factor, TAF2H, Mus musculus domesticus, TAF2A, Factor GATA1, Eryf1 Erythroid-Specific Transcription Factor, Mice, Mus musculus, Transcription, Transcription Factor NFE-1, red blood cell differentiation, Caspase-14 subunit p10, Eryf1 Erythroid Specific Transcription Factor, Swiss, Erythropoieses, GATA1, mice, Swiss Mouse, House Mice, Swiss Mice, Caspase-14 subunit p19, \"mouse\" EXACT common_name [], MICE, Laboratory Mice, domesticus, \"house mouse\" EXACT genbank_common_name [], \"Mus muscaris\" RELATED misnomer [], Taf2h, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Nuclear Factor-Erythroid 1, Mouse, house mouse, Laboratory Mouse"],"pubmed_abstract_synonyms":["phosphoric acid tributyl ester, 3.4.22.-, transcription, GTF2D, TAFII30, ERYF1, Materials, human being, single-organism developmental process, Laboratory, single-organism developmental process., Mus domesticus, Gene, mini-ICE, pre-integration complex, CASP-14, CSMF, Spectrum Analyses, NF-E1 DNA-binding protein, Homo sapiense, composed of, House Mouse, General Transcription Factors, Homo spaiens, AU041226, Homo sapien, conclusions, House, 30kDa, Homo sapians, Mass, symptoms, TAF2H, Mus musculus domesticus, eryf1, GF1, transcription regulator activity, Gtf2d, TAF2A, Analysis, Mice, Gata1, Mass Spectroscopy, Mass Spectrum Analysis, HDL4, Homo sapients, Genetic, GATA-1, Analyses, AI573420, Erythropoieses, Swiss, cell, GATA1, ligand, composition, Swiss Mice, proteins, \"mouse\" EXACT common_name [], man, XLTT, NFE1, \"Mus muscaris\" RELATED misnomer [], Taf2h, DNA-dependent, NOR-1, SCA17, DNA Dependent RNA Polymerase II, Homo sapience, Erythroid Cell, PIC, Homo sampiens, GTF2D1, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], house mouse, associated, GATA-binding factor 1, Home sapiens, Differentiation, Gata-1, serum total protein, screening, cellular transcription, NOR1, data, Nor1, findings, CHN, DNA-dependent transcription, transcription preinitiation complex, tri-(n-butyl )-phosphate, preinitiation complex, mouse, compositionality, Cistrons, erythropoiesis, Spectrum Analysis, Cell, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Spectroscopy, polypeptide, development, TBP, XLTDA, batch, NF-E1, Mus, Homo sapian, erythrocyte cell differentiation, Mini-ICE, Genetic Materials, TFIID, Erythroid, concluding remarks, XLANP, Homo sapeins, Genetic Material, Mass Spectrum Analyses, Differentiations, General Transcription Initiation Complex Proteins, Gf-1, Mass Spectrum, Mus musculus, red blood cell differentiation, Caspase-14 subunit p10, tri-n-butyl phosphate, tributylphosphate, MINOR, RNA Pol II, DNA-templated transcriptional 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Minor"],"view_count":["32"],"citation_count":["0"],"search_count":["7"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015002"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~1","opentargets~0","efo~0","embl-pathogen~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","project~1","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride 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Experiment: hs, file: F049331.dat-pride.pride.mgf. Published as part of Mol Cell Biol. 2015 Apr 13  . From the Abstract: {{i}} The ordered assembly of a functional pre-initiation complex (PIC), composed of general transcription factors (GTFs), is a prerequisite for the transcription of protein-coding genes by RNA polymerase II. TFIID, comprised of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is the GTF that is thought to recognize the promoter sequences allowing site-specific PIC assembly. Transcriptional cofactors, such as SAGA, are also necessary for tightly regulated transcription initiation. The contribution of the two TAF10-containing complexes (TFIID, SAGA) to erythropoiesis remains elusive. By ablating TAF10 specifically in erythroid cells in vivo we observed a differentiation block accompanied by deregulated GATA1 target genes, including Gata1 itself, suggesting functional crosstalk between GATA1 and TAF10. Additionally, we analyzed the composition of TFIID and SAGA complexes by mass spectrometry in mouse and human cells and found that their global integrity is maintained, with minor changes, during erythroid differentiation and development ... {{/i}}","dates":{"submission":"2015-04-29"},"accession":"GPM32320015002","cross_references":{"pubmed":["25870109"],"Pride":["PXD000729"],"pride":[],"Pride Archive":["PXD000729"]}}