GPMDB00195OtherChang HY, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_femalehsinyi.chang@gmail.com25556991Department of Life Science, National Taiwan Universityhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015984Not availableGPMDBBreast cancer is one of the leading cancer-related causes of death worldwide. Treatment of triple-negative breast cancer (TNBC) is complex and challenging, especially when metastasis has developed. In this study, we applied infrared radiation as an alternative approach for the treatment of TNBC. We used middle infrared (MIR) with a wavelength range of 3-5 μm to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells but did not affect the growth of normal breast epithelial cells. We performed iTRAQ-coupled LC-MS/MS analysis to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1749 proteins were identified, quantified, and subjected to functional enrichment analysis. From the constructed functionally enriched network, we confirmed that MIR caused G2/M cell cycle arrest, remodeled the microtubule network to an astral pole arrangement, altered the actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Our results reveal the coordinative effects of MIR-regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy.Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells.Chang Hsin-Yi HY,Li Ming-Hua MH,Huang Tsui-Chin TC,Hsu Chia-Lang CL,Tsai Shang-Ru SR,Lee Si-Chen SC,Huang Hsuan-Cheng HC,Juan Hsueh-Fen HF,Chang Hsin-Yi HY, Li Ming-Hua MH, Huang Tsui-Chin TC, Hsu Chia-Lang CL, Tsai Shang-Ru SR, Lee Si-Chen SC, Huang Hsuan-Cheng HC, Juan Hsueh-Fen HFRadiations, Carcinomas, BC, Mammary Neoplasms, Human Mammary Neoplasm, Carcinoma, Carcinoma of breast NOS, Breast Neoplasm, Human Mammary, Breast Neoplasms, NEOPL BREAST, Mammary Neoplasm, Carcinoma of breast NOS (disorder), Neoplasms, Mammary Carcinoma, carcinoma OF breast, number, Human Mammary Carcinomas, Human Mammary Neoplasms, Tumor, presence, Cancer of the Breast, Human, CA - Carcinoma of breast, count in organism, count, Mammary Carcinomas, Breast Tumor, BREAST NEOPL, Breast Tumors, Breast Cancer, Neoplasm, Carcinoma of breast (disorder), Human Mammary Carcinoma, Cancer of Breast, Cell., Carcinoma of the Breast, quantitative, Breast, Mammary carcinoma, presence or absence in organism, Tumors, Cancerliquid chromatography tandem mass spectroscopy, mammary region, Carcinoma of breast NOS, Breasts, Epithelial Cell, determination, Metastasis, Breast Neoplasms, Neoplasms, Human Mammary Neoplasms, Neoplasm Metastases, Glandular Epithelial, Tumor, Glandular, IDOL, Cell Growth in Number, LC-MS-MS, Human, Squamous Epithelial Cells, Breast Tumor, BREAST NEOPL, LC-MSMS, Cell Number Growth, Mood, Cell., Carcinoma of the Breast, MIR, Mir, Number Growth, Squamous Epithelial, Epithelial, Normalities, treatment, BC, average, Triple-Negative Breast Carcinoma, study, Mammary Neoplasms, Human Mammary, Growth, TN breast cancer, LCMSMS, MONDOA, Mammary Neoplasm, Carcinoma of breast NOS (disorder), Moods, N, Mammary Carcinoma, mammary part of chest, Human Mammary Carcinomas, Adenomatous Epithelial, Cancer of the Breast, CA - Carcinoma of breast, cell proliferation, Myosin regulatory light chain-interacting protein, Epithelial Cells, Transitional Epithelial Cells, disease management, Carcinoma of breast (disorder), bHLHe36, Breast, Squamous Epithelial Cell, Idol, Glandular Epithelial Cell, Tumors, Cancer, 9430057C20Rik, mamma, Transitional Epithelial Cell, Transitional, LC-MS2, Multiplication, Human Mammary Neoplasm, Carcinoma, Squamous Cells, data, Cellular Proliferation, Myosin regulatory light chain interacting protein, 6.3.2.-, Affects, Metastases, LC-MS/MS, Adenomatous, Normalcy, Squamous, Transitional Epithelial, Cell, AW228700, LC/MS/MS, Cuboidal Glandular Epithelial Cells, Mammary Carcinomas, chemical analysis, Neoplasm, Human Mammary Carcinoma, Cellular, Columnar Glandular Epithelial Cells, Mammary carcinoma, Radiations, Carcinomas, Adenomatous Epithelial Cell, Adenomatous Epithelial Cells, Breast Neoplasm, Squamous Cell, growth pattern, Normality, NEOPL BREAST, non-developmental growth, carcinoma OF breast, Glandular Epithelial Cells, Inducible degrader of the LDL-receptor, Health, Proliferation, liquid chromatography-tandem mass spectroscopy, Breast Tumors, Breast Cancer, Cell Multiplication, Cells, Cell Number, liquid chromatography tandem mass spectrometry, Cancer of Breast, assay, ProteomesRadiations, Carcinomas, BC, Mammary Neoplasms, Human Mammary Neoplasm, Carcinoma, Carcinoma of breast NOS, Breast Neoplasm, Human Mammary, Breast Neoplasms, NEOPL BREAST, Mammary Neoplasm, Carcinoma of breast NOS (disorder), Neoplasms, Mammary Carcinoma, carcinoma OF breast, number, Human Mammary Carcinomas, Human Mammary Neoplasms, Tumor, presence, Cancer of the Breast, Human, CA - Carcinoma of breast, count in organism, count, Mammary Carcinomas, Breast Tumor, BREAST NEOPL, Breast Tumors, Breast Cancer, Neoplasm, Carcinoma of breast (disorder), Human Mammary Carcinoma, Cancer of Breast, Cell., Carcinoma of the Breast, quantitative, Breast, Mammary carcinoma, presence or absence in organism, Tumors, Cancerliquid chromatography tandem mass spectroscopy, Focal Adhesion, deceased, Breasts, Epithelial Cell, determination, Metastasis, Breast Neoplasms, Human Mammary Neoplasms, Neoplasm Metastases, Cardiac Death, neurotubule, Adherens Junctions, Tumor, Glandular, IDOL, Cell Growth in Number, Cell Number Growth, establishment and maintenance of substrate location, Focal Contact, MIR, Mir, present in fewer numbers in organism, microtubulus, multicellular organismal biosynthetic process, BC, treatment, average, Mammary Neoplasms, adhesion plaque, hemi-adherens junction, single-organism biosynthetic process, Growth, LCMSMS, Focal, Moods, anabolism, Cell-Matrix Adherens Junctions, N, Human Mammary Carcinomas, hypoplasia, Adenomatous Epithelial, proteins, CA - Carcinoma of breast, Adhesions, decreased, Adhesion, disease management, Carcinoma of breast (disorder), Adherens Junction, microtubuli, Contacts, Adhesion Plaques, Idol, Adhesion Plaque, Tumors, Plaque, 9430057C20Rik, mamma, Transitional Epithelial Cell, LC-MS2, establishment and maintenance of substance location, Carcinoma, 6.3.2.-, Focal Contacts, LC-MS/MS, Cell-Matrix, Adenomatous, Normalcy, Squamous, Transitional Epithelial, results, single organism localization, Cell-Matrix Adherens Junction, Cellular, Near-Death Experience, Mammary carcinoma, Microtubule, Carcinomas, Adenomatous Epithelial Cell, Adenomatous Epithelial Cells, Squamous Cell, growth pattern, Determination of Death, NEOPL BREAST, non-developmental growth, cessation of cell cycle, carcinoma OF breast, Inducible degrader of the LDL-receptor, Junction, Cell-Matrix Adherens, Plaques, Health, Proliferation, liquid chromatography-tandem mass spectroscopy, Breast Tumors, Cells, Cell Number, establishment and maintenance of cellular component location, mammary region, Carcinoma of breast NOS, Neoplasms, Gene, arrest of cell cycle progression, Glandular Epithelial, biosynthesis, LC-MS-MS, Human, Cardiac, Squamous Epithelial Cells, reduced, Breast Tumor, termination of cell cycle, subnumerary, BREAST NEOPL, LC-MSMS, Gene Products, establishment and maintenance of position, Mood, Carcinoma of the Breast, tiny, Number Growth, Squamous Epithelial, malignant tumour, Epithelial, Normalities, Triple-Negative Breast Carcinoma, study, Human Mammary, TN breast cancer, MONDOA, formation, Mammary Neoplasm, Carcinoma of breast NOS (disorder), Mammary Carcinoma, mammary part of chest, malignant neoplasia, decreased number, Junctions, Cancer of the Breast, focal contact, synthesis, cell proliferation, Myosin regulatory light chain-interacting protein, Epithelial Cells, Transitional Epithelial Cells, bHLHe36, CRCS12, Breast, Squamous Epithelial Cell, Glandular Epithelial Cell, Cancer, Transitional, small, Multiplication, Human Mammary Neoplasm, Squamous Cells, Cellular Proliferation, Malignant Neoplasm, Myosin regulatory light chain interacting protein, Affects, Metastases, Proteins, Cell, AW228700, polypeptide, LC/MS/MS, disease management., Cuboidal Glandular Epithelial Cells, Mammary Carcinomas, FILS, chemical analysis, Protein, post-mortem, Neoplasm, Human Mammary Carcinoma, establishment and maintenance of localization, Columnar Glandular Epithelial Cells, Death, Radiations, death, Cell Matrix Adherens Junctions, Breast Neoplasm, underdeveloped, Normality, Glandular Epithelial Cells, Cancers, single-organism localization, malignant tumor, Protein Gene Products, Gene Proteins, localisation, microfilament, Breast Cancer, Cell Multiplication, Contact, liquid chromatography tandem mass spectrometry, Cancer of Breast, assay, POLE11905http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320015984dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0058605798889574340.01.0falseQuantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells.Data from ProteomeXchange, PXD ID: PXD001078. File: folder summary. Published as part of J Proteome Res. 2015 Feb 6;14(2):1250-62 . From the Abstract: {{i}} ... Treatment of triple-negative breast cancer (TNBC) is complex and challenging, especially when metastasis has developed. In this study, we applied infrared radiation as an alternative approach for the treatment of TNBC. We used middle infrared (MIR) with a wavelength range of 3-5 microns to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells but did not affect the growth of normal breast epithelial cells. We performed iTRAQ-coupled LC-MS/MS analysis to investigate the MIR-triggered molecular mechanisms in breast cancer cells ... {{/i}}2015-06-24GPM3232001598425556991PXD001078PXD001078