GPMDB00425OtherEravci M, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Human_female24791982matthias.selbach@mdc-berlin.dehttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016240http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016238http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016253http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016231http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016252http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016233http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016255http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016298http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016232http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016254http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016235http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016257http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016234http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016256http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016259http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016237http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016236http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016258http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016291http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016251http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016250http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016228http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016249http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016227http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016229http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016242http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016285http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016241http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016244http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016243http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016246http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016245http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016248http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016226http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016247http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016225Institute of Chemistry and Biochemistry, Freie Universitaet BerlinNot availableGPMDBEfficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible.IPG strip-based peptide fractionation for shotgun proteomics.Eravci Murat M,Sommer Christian C,Selbach Matthias M,Eravci Murat M, Sommer Christian C, Selbach Matthias Mpeptide, polypeptide.flavanone synthase activity, data, malonyl-CoA:4-coumaroyl-CoA malonyltransferase (cyclizing), Procedures, determination, mol, CHS, Spectrum Analyses, Procedure, DOCS, Spectrum Analysis, Spectroscopy, polypeptide, peptide, Polypeptides, method, Experiment, Method, method used in an experiment, chemical analysis, flavonone synthase activity, Studies, Mass, Chédiak-Higashi disease, chalcone synthase activity, techniques, Analysis, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, chalcone synthetase activity, Mass Spectrum, Chédiak-Higashi-Steinbrink syndrome, Analyses, Electrofocusing, Spectrometry, proteins, Focusing, present in organism, Methodological, procedures, Isoelectric, study protocol, Methodological Study, sample population, HPLC, plan specification, Study, sample., Methodological Studies, Chediak - Steinbrinck anomaly, Chediak-Higashi syndrome, assay, methodologypeptide, polypeptide.Mass Spectrum Analysis, Mass Spectrum, Procedures, determination, Analyses, Electrofocusing, Spectrometry, proteins, Focusing, present in organism, Methodological, Spectrum Analyses, Isoelectric, Procedure, study protocol, Methodological Study, Spectrum Analysis, sample population, HPLC, plan specification, Spectroscopy, Study, polypeptide, peptide, Polypeptides, sample., method, Methodological Studies, Method, method used in an experiment, chemical analysis, Mass, Studies, assay, Analysis, Mass Spectrum Analyses, Mass Spectroscopy4205http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016255dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0129549660703269580.01.0falseIPG strip-based peptide fractionation for shotgun proteomics.Data from ProteomeXchange, PXD ID: PXD002389. Experiment: Bibo_20130621_CHS_IEF_3-10linear_24slices, file: Bibo_20130621_CHS_IEF_3-10linear_24slices_20.mzml. Published as part of Methods Mol Biol. 2014;1156:67-77 . From the Abstract: {{i}} Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible. {{/i}}2015-07-10GPM3232001625524791982PXD002389PXD002389