GPMDB00248OtherJumeau F, et al.InstrumentNot AvailableNot availableHomo_sapiens_viruses, Humanmelanie.lagarrigue@univ-rennes1.fr26168773Protim, Campus de Beaulieu, 35042 Rennes Cedex, Francehttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016361http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016360http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016359http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016396http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016352http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016351http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016395http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016354http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016398http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016353http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016397http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016356http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016355http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016399http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016358http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016357http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016370http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016372http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016371http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016404http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016403http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016406http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016405http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016408http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016407http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016409http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016363http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016362http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016365http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016364http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016400http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016367http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016366http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016369http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016402http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016368http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016401http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016381http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016380http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016383http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016382http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016374http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016373http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016376http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016375http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016378http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016411http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016377http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016410http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016413http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016412http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016379http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016390http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016391http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016394http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016393http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016385http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016384http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016387http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016386http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016389http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016388Not availableGPMDBThe Chromosome-Centric Human Proteome Project (C-HPP) aims at cataloguing the proteins as gene products encoded by the human genome in a chromosome-centric manner. The existence of products of about 82% of the genes has been confirmed at the protein level. However, the number of so-called "missing proteins" remains significant. It was recently suggested that the expression of proteins that have been systematically missed might be restricted to particular organs or cell types, for example, the testis. Testicular function, and spermatogenesis in particular, is conditioned by the successive activation or repression of thousands of genes and proteins including numerous germ cell- and testis-specific products. Both the testis and postmeiotic germ cells are thus promising sites at which to search for missing proteins, and ejaculated spermatozoa are a potential source of proteins whose expression is restricted to the germ cell lineage. A trans-chromosome-based data analysis was performed to catalog missing proteins in total protein extracts from isolated human spermatozoa. We have identified and manually validated peptide matches to 89 missing proteins in human spermatozoa. In addition, we carefully validated three proteins that were scored as uncertain in the latest neXtProt release (09.19.2014). A focus was then given to the 12 missing proteins encoded on chromosomes 2 and 14, some of which may putatively play roles in ciliation and flagellum mechanistics. The expression pattern of C2orf57 and TEX37 was confirmed in the adult testis by immunohistochemistry. On the basis of transcript expression during human spermatogenesis, we further consider the potential for discovering additional missing proteins in the testicular postmeiotic germ cell lineage and in ejaculated spermatozoa. This project was conducted as part of the C-HPP initiatives on chromosomes 14 (France) and 2 (Switzerland). The mass spectrometry proteomics data have been deposited with the ProteomeXchange Consortium under the data set identifier PXD002367.Human Spermatozoa as a Model for Detecting Missing Proteins in the Context of the Chromosome-Centric Human Proteome Project.Jumeau Fanny F,Com Emmanuelle E,Lane Lydie L,Duek Paula P,Lagarrigue Mélanie M,Lavigne Régis R,Guillot Laëtitia L,Rondel Karine K,Gateau Alain A,Melaine Nathalie N,Guével Blandine B,Sergeant Nicolas N,Mitchell Valérie V,Pineau Charles C,Jumeau Fanny F, Com Emmanuelle E, Lane Lydie L, Duek Paula P, Lagarrigue Mélanie M, Lavigne Régis R, Guillot Laëtitia L, Rondel Karine K, Gateau Alain A, Melaine Nathalie N, Guével Blandine B, Sergeant Nicolas N, Mitchell Valérie V, Pineau Charles CY-Chromosome-Bearing, X-Bearing Sperm, Sperm, human being, Proteins, Gene, Homo sapiense, Y-Chromosome-Bearing Sperm, polypeptide, Homo spaiens, prophase chromosome, Homo sapien, Homo sapian, Homo sapians, Protein, Proteomes., Spermatozoon, Gene Products, Homo sapeins, Y-Bearing, Y Bearing Sperm, X-Chromosome-Bearing Sperm, centric, X-Chromosome-Bearing, X-Bearing, Homo sapients, interphase chromosome, Humo sapiens, proteins, chromatid, X-Bearing Sperms, man, Homo sapines, X Bearing Sperm, human, X-Chromosome-Bearing Sperms, Protein Gene Products, Gene Proteins, Y Chromosome Bearing Sperm, Y-Bearing Sperms, Homo spiens, "human" EXACT genbank_common_name [], Homo sapience, Y-Bearing Sperm, Chromosome, X Chromosome Bearing Sperm, Homo sampiens, Sperms, Y-Chromosome-Bearing Sperms, Home sapiensSpermiogenesis, Sperm, human being, Materials, determination, testicle, number, male organism reproductive system gonad, Gene, Homo sapiense, Y-Chromosome-Bearing Sperm, Germ Lines, Human, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, Homo spaiens, male genitalia gonada, Germ Line, Homo sapien, male reproductive system gonad, Homo sapians, SPH3, gonad of male genitalia, Gene Products, Spermatozoon, Germ Line Cells, testiculus, Y Bearing Sperm, X-Chromosome-Bearing Sperms., Gametes, X-Chromosome-Bearing Sperm, X-Bearing, Homo sapients, Genetic, HPP, Genomes, cell, Testicle, gonada of genitalia of male organism, gonada of male organism reproductive system, Rathburn disease, gonad of male organism genitalia, chromatid, proteins, X-Bearing Sperms, number of, Cell Lineages, man, gonad of reproductive system of male organism, Germ-Line, X-Chromosome-Bearing Sperms, Y Chromosome Bearing Sperm, Human Genomes, Homo sapience, Y-Bearing Sperm, EL2, X Chromosome Bearing Sperm, Homo sampiens, orchis, Y-Chromosome-Bearing Sperms, has or lacks parts of type, male genitalia gonad, testes, male organism genitalia gonada, Home sapiens, Y-Chromosome-Bearing, data, X-Bearing Sperm, HS3, reproductive system of male organism gonad, Proteins, gonada of male genitals, gonad of male organism reproductive system, Spermatocytogenesis, Germ-Line Cell, extra or missing physical or functional parts, function, Germ, gonad of male genitals, Cistrons, Cell, genitalia of male organism gonad, mereological quality, polypeptide, prophase chromosome, generation of spermatozoa, male genitals gonad, Lineage, Human Genome, Homo sapian, gonad of genitalia of male organism, SPTA, Protein, gamete, chemical analysis, Genetic Materials, Germ Cell, Homo sapeins, genitalia of male organism gonada, Y-Bearing, Genetic Material, activation, gonada of reproductive system of male organism, Entire testis, centric, Germ-Line Cells, X-Chromosome-Bearing, gonada of male reproductive system, interphase chromosome, Lineages, Humo sapiens, male gonad, Phosphoethanolaminuria, Homo sapines, X Bearing Sperm, human, male reproductive system gonada, Protein Gene Products, Gene Proteins, Y-Bearing Sperms, Homo spiens, "human" EXACT genbank_common_name [], Chromosome, Material, Sperms, cardinality, Testicles, Cells, Cistron, assay, male organism reproductive system gonada, gonada of male genitalia, male organism genitalia gonad, Proteomes, TestesY-Chromosome-Bearing, X-Bearing Sperm, Sperm, human being, Proteins, Gene, Homo sapiense, Y-Chromosome-Bearing Sperm, polypeptide, Homo spaiens, prophase chromosome, Homo sapien, Homo sapian, Homo sapians, Protein, Proteomes., Spermatozoon, Gene Products, Homo sapeins, Y-Bearing, Y Bearing Sperm, X-Chromosome-Bearing Sperm, centric, X-Chromosome-Bearing, X-Bearing, Homo sapients, interphase chromosome, Humo sapiens, proteins, chromatid, X-Bearing Sperms, man, Homo sapines, X Bearing Sperm, human, X-Chromosome-Bearing Sperms, Protein Gene Products, Gene Proteins, Y Chromosome Bearing Sperm, Y-Bearing Sperms, Homo spiens, "human" EXACT genbank_common_name [], Homo sapience, Y-Bearing Sperm, Chromosome, X Chromosome Bearing Sperm, Homo sampiens, Sperms, Y-Chromosome-Bearing Sperms, Home sapiensMaterials, determination, testicle, adult stage, male organism reproductive system gonad, primary cilium, Homo sapiense, Technic, peptide, Techniques, TSC21, Homo sapien, Immunogold-Silver Techniques, Homo sapians, Immunogold-Silver Technique, SPH3, gonad of male genitalia, C2orf51, Analysis, adult, X-Chromosome-Bearing Sperm, Mass Spectrum Analysis, X-Bearing, Homo sapients, HPP, Genomes, Analyses, Testicle, gonada of male organism reproductive system, Rathburn disease, chromatid, proteins, number of, Germ-Line, Y Chromosome Bearing Sperm, Immunogold-Silver Technic, Human Genomes, Homo sapience, Y-Bearing Sperm, EL2, X Chromosome Bearing Sperm, Y-Chromosome-Bearing Sperms, male genitalia gonad, Immunolabeling Technique, Home sapiens, Immunogold Technics, Data Set., Y-Chromosome-Bearing, HS3, reproductive system of male organism gonad, gonada of male genitals, Immunogold Technique, Germ-Line Cell, extra or missing physical or functional parts, gonad of male genitals, INSDC_feature:misc_RNA, Spectrum Analysis, Spectroscopy, Immunogold, Immunogold Techniques, RGD1562720, Immunolabeling Technic, Playthings and Play, Homo sapian, gamete, Immunolabeling Technics, Plaything, Genetic Materials, Germ Cell, Technics, Adults, Genetic Material, activation, gonada of reproductive system of male organism, Entire testis, X-Chromosome-Bearing, adults, Toys, Spectrometry, male gonad, Immunogold Silver Techniques, Homo sapines, X Bearing Sperm, Saint Pierre and Miquelon, human, Chromosome, Material, Sperms, Testicles, Cells, Cistron, St. Pierre and Miquelon, male organism reproductive system gonada, male organism genitalia gonad, Proteomes, Immunogold-Silver, Spermiogenesis, Sperm, Plays, human being, number, Gene, flagellum, Spectrum Analyses, Y-Chromosome-Bearing Sperm, Germ Lines, Human, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, Homo spaiens, male genitalia gonada, Germ Line, male reproductive system gonad, Miquelon and St. Pierre, Gene Products, Spermatozoon, Mass, Germ Line Cells, testiculus, Y Bearing Sperm, Technique, Immunogold Silver Technics, Mass Spectroscopy, eukaryotic flagellum, Toy, Gametes, FOCUS, Genetic, pattern, Playthings, cell, distribution, gonada of genitalia of male organism, gonad of male organism genitalia, X-Bearing Sperms, man, gonad of reproductive system of male organism, X-Chromosome-Bearing Sperms, Miquelon and Saint Pierre, Puppets, Homo sampiens, orchis, has or lacks parts of type, Play, testes, male organism genitalia gonada, microtubule-based flagellum, Puppet, X-Bearing Sperm, data, Flagellum, Immunogold Technic, Proteins, gonad of male organism reproductive system, Immunohistocytochemistry, Spermatocytogenesis, function, Germ, Cistrons, Cell, genitalia of male organism gonad, mereological quality, polypeptide, prophase chromosome, generation of spermatozoa, male genitals gonad, Human Genome, gonad of genitalia of male organism, SPTA, Protein, chemical analysis, Homo sapeins, genitalia of male organism gonada, Y-Bearing, Mass Spectrum Analyses, Corsica, Immunolabeling, centric, Germ-Line Cells, Mass Spectrum, Immunocytochemistry, gonada of male reproductive system, interphase chromosome, Humo sapiens, Phosphoethanolaminuria, male reproductive system gonada, Protein Gene Products, 1700011F03Rik, Gene Proteins, Y-Bearing Sperms, Homo spiens, "human" EXACT genbank_common_name [], cardinality, Immunogold-Silver Technics, Immunolabeling Techniques, assay, gonada of male genitalia, Testes2408http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320016359dbgap_ncbi~0patentfamilies~0rfam~0uniprot~2merops~0complex-portal~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0sc-genes~0hgnc~1intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0074028377544725481.00.0falseHuman spermatozoa as a model for detecting missing proteins in the context of the chromosome-centric Human Proteome Project.Data from ProteomeXchange, PXD ID: PXD002367. File: Mitchell_40_F03_exclu02.mzml. Published as part of J Proteome Res. 2015 . From the Abstract: {{i}} The chromosome-centric Human Proteome Project (C-HPP) aims at cataloguing the proteins as gene products encoded by the human genome, in a chromosome-centric manner. The existence of products of about 82% of the genes has been confirmed at the protein level. However, the number of so-called missing proteins remains significant. It was recently suggested that the expression of proteins that have been systematically missed might be restricted to particular organs or cell types, for example the testis. Testicular function, and spermatogenesis in particular, is conditioned by the successive activation and/or repression of thousands of genes and proteins, including numerous germ cell- and testis-specific products. Both the testis and post-meiotic germ cells are thus promising sites at which to search for missing proteins and ejaculated spermatozoa are a potential source of proteins whose expression is restricted to the germ cell lineage. A trans-chromosome-based data analysis was performed to catalog missing proteins in total protein extracts from isolated human spermatozoa ... {{/i}}2015-07-16GPM3232001635926168773PXD002367PXD002367