GPMDB00145OtherZheng B, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mouseguo_xuejiang@njmu.edu.cn26199344Nanjing Medical Universityhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017393Not availableGPMDBTesticular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With the high-throughput quantitative proteomics technology, a comparative proteomic profile of mouse embryonic male gonads were analyzed at three time points (11.5, 12.5, and 13.5 days post coitum), corresponding to critical stages of testicular cord formation in gonadal development. 4070 proteins were identified, and 338 were differentially expressed, of which the Sertoli cell specific genes were significant enrichment, with mainly increased expression across testis cord development. Additionally, we found overrepresentation of proteins related to oxidative stress in these Sertoli cell specific genes. Of these differentially expressed oxidative stress-associated Sertoli cell specific protein, stromal interaction molecule 1, was found to have discrepant mRNA and protein regulations, with increased protein expression but decreased mRNA levels during testis cord development. Knockdown of Stim1 in Sertoli cells caused extensive defects in gonadal development, including testicular cord disruption, loss of interstitium, and failed angiogenesis, together with increased levels of reactive oxygen species. And suppressing the aberrant elevation of reactive oxygen species could partly rescue the defects of testicular cord development. Taken together, our results suggest that reactive oxygen species regulation in Sertoli cells is important for gonadogenesis, and the quantitative proteomic data could be a rich resource to the elucidation of regulation of testicular cord development.Quantitative Proteomics Reveals the Essential Roles of Stromal Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in Mouse Testis.Zheng Bo B,Zhao Dan D,Zhang Pan P,Shen Cong C,Guo Yueshuai Y,Zhou Tao T,Guo Xuejiang X,Zhou Zuomin Z,Sha Jiahao J,Zheng Bo B, Zhao Dan D, Zhang Pan P, Shen Cong C, Guo Yueshuai Y, Zhou Tao T, Guo Xuejiang X, Zhou Zuomin Z, Sha Jiahao JTAM, 3.4.22.-, reproductive system of male organism gonad, Laboratory, testicle, Mus domesticus, gonada of male genitals, number, mouse, male organism reproductive system gonad, gonad of male organism reproductive system, mini-ICE, TAM1, CASP-14, IMD10, biosynthesis, gonad of male genitals, presence, House Mouse, genitalia of male organism gonad, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, count in organism, male genitalia gonada, count, male genitals gonad, House, Mus, gonad of genitalia of male organism, male reproductive system gonad, Mini-ICE, gonad of male genitalia, Mus musculus domesticus, SIM, testiculus, Mice, genitalia of male organism gonada, gonada of reproductive system of male organism, multicellular organismal biosynthetic process, Mus musculus, D11S4896E, single-organism biosynthetic process, Caspase-14 subunit p10, formation, gonada of male reproductive system, Swiss, Testicle, anabolism, mice, gonada of genitalia of male organism, gonada of male organism reproductive system, Swiss Mouse, male gonad, gonad of male organism genitalia, House Mice, Swiss Mice, STRMK, Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, gonad of reproductive system of male organism, Laboratory Mice, domesticus, male reproductive system gonada, synthesis, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], GOK, orchis, Testicles, male genitalia gonad, Entire testis., "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, quantitative, house mouse, male organism reproductive system gonada, testes, gonada of male genitalia, male organism genitalia gonada, male organism genitalia gonad, Laboratory Mouse, Testes, presence or absence in organismmale genital system, 3.4.22.-, data, mol, Laboratory, Effects, Males, single-organism developmental process., Arts, Mus domesticus, number, mouse, Longterm Effect, mini-ICE, Gonad, CASP-14, biosynthesis, http://mged.sourceforge.net/ontologies/MGEDOntology.owl#male, presence, House Mouse, Cell, mice C57BL/6xCBA/CaJ hybrid, Long Term, Mus muscaris, period, development, count in organism, Experiment, count, House, Mus, Mini-ICE, Long Term Effects, Mus musculus domesticus, Mice, Effect, multicellular organismal biosynthetic process, male, Embryonic, Mus musculus, single-organism biosynthetic process, Industrial, Industrial Arts, Caspase-14 subunit p10, gonada, formation, Longterm, cell, Swiss, anabolism, mice, Swiss Mouse, male genitalia, House Mice, Swiss Mice, Caspase-14 subunit p19, Long-Term, "mouse" EXACT common_name [], MICE, Longterm Effects, Laboratory Mice, domesticus, synthesis, male human body, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], gonadogenesis, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, Gonadal, Long-Term Effect, quantitative, house mouse, gonads, Laboratory Mouse, Long-Term Effects, time, Gonads, presence or absence in organismTAM, 3.4.22.-, reproductive system of male organism gonad, Laboratory, testicle, Mus domesticus, gonada of male genitals, number, mouse, male organism reproductive system gonad, gonad of male organism reproductive system, mini-ICE, TAM1, CASP-14, IMD10, biosynthesis, gonad of male genitals, presence, House Mouse, genitalia of male organism gonad, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, gonada of male organism genitalia, gonad of male reproductive system, reproductive system of male organism gonada, count in organism, male genitalia gonada, count, male genitals gonad, House, Mus, gonad of genitalia of male organism, male reproductive system gonad, Mini-ICE, gonad of male genitalia, Mus musculus domesticus, SIM, testiculus, Mice, genitalia of male organism gonada, gonada of reproductive system of male organism, multicellular organismal biosynthetic process, Mus musculus, D11S4896E, single-organism biosynthetic process, Caspase-14 subunit p10, formation, gonada of male reproductive system, Swiss, Testicle, anabolism, mice, gonada of genitalia of male organism, gonada of male organism reproductive system, STIM1 Protein, Swiss Mouse, male gonad, gonad of male organism genitalia, House Mice, Swiss Mice, STRMK, Caspase-14 subunit p19, "mouse" EXACT common_name [], MICE, gonad of reproductive system of male organism, Laboratory Mice, domesticus, male reproductive system gonada, synthesis, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], GOK, orchis, Testicles, male genitalia gonad, Entire testis., "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, quantitative, house mouse, male organism reproductive system gonada, testes, gonada of male genitalia, male organism genitalia gonada, male organism genitalia gonad, Laboratory Mouse, Testes, presence or absence in organism3.4.22.-, Sertoli, atypia, Materials, single-organism developmental process, Laboratory, Effects, single-organism developmental process., Mus domesticus, number, primary sex cord, Gene, mini-ICE, Gonad, CASP-14, biosynthesis, http://mged.sourceforge.net/ontologies/MGEDOntology.owl#male, presence, House Mouse, supernumerary, Long Term, Stresses, period, reduced, House, Oxidative, subnumerary, Gene Products, Mus musculus domesticus, atypical, testis primitive sex cord, Mice, Effect, present in fewer numbers in organism, Reactive, multicellular organismal biosynthetic process, male, increased, single-organism biosynthetic process, D11S4896E, Genetic, gonada, formation, Longterm, cell, Swiss, anabolism, male genitalia, messenger RNA, Swiss Mice, proteins, Long-Term, decreased number, "mouse" EXACT common_name [], Oxygen Species, synthesis, "Mus muscaris" RELATED misnomer [], GOK, Oxygen, template RNA, Oxidative Stresses, decreased, blood vessel formation from pre-existing blood vessels, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Gonadal, Long-Term Effect, house mouse, associated, gonads, Active Oxygen, Long-Term Effects, male genital system, TAM, data, Pro Oxidants, aberrant, Males, Arts, Proteins, mouse, Longterm Effect, interstitium, testis primary sex cords, TAM1, IMD10, defective, Cistrons, Cell, results, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, development, polypeptide, count in organism, count, Mus, Mini-ICE, Long Term Effects, Protein, INSDC_feature:mRNA, Genetic Materials, Active, SIM, Oxygen Radicals, Genetic Material, Embryonic, Mus musculus, Industrial, Industrial Arts, protein_coding_transcript, Caspase-14 subunit p10, mRNA, mice, increased number, STIM1 Protein, Swiss Mouse, House Mice, Caspase-14 subunit p19, STRMK, MICE, Longterm Effects, Laboratory Mice, domesticus, male human body, Protein Gene Products, loss of, "house mouse" EXACT genbank_common_name [], Gene Proteins, present in greater numbers in organism, Material, Stress, Cells, gonadogenesis, Pro-Oxidants, Cistron, Mouse, regulation, quantitative, Laboratory Mouse, time, testis cord, Gonads, accessory, presence or absence in 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archive~1varsite~00Resource Reanalysis0.00.00.004318322023442320.01.0falseQuantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis.Data from ProteomeXchange, PXD ID: PXD002563. Experiment: group1, file: folder summary. Published as part of Mol Cell Proteomics. 2015 Jul 21 . From the Abstract: {{i}} Testicular cord formation in male gonadogenesis involves assembly of several cell types, the precise molecular mechanism is still not well known. With the high-throughput quantitative proteomics technology, a comparative proteomic profile of mouse embryonic male gonads were analyzed at three time points (11.5, 12.5 and 13.5 days post coitum), corresponding to critical stages of testicular cord formation in gonadal development ... {{/i}}2015-07-30GPM3232001739326199344PXD002563PXD002563