GPMDB00123OtherCardona M, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mousejalopez@cnic.es26121671Departament de Ciencies Mediques Basiques, Universitat de Lleida-IRBLLEIDAhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017673http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017674http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017672http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017677http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017678http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017675http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017676Not availableGPMDBExecutioner caspase-3 and -7 are proteases promoting cell death but non-apoptotic roles are being discovered. The heart expresses caspases only during development, suggesting they contribute to the organ maturation process. Therefore, we aimed at identifying novel functions of caspases in heart development. We induced simultaneous deletion of executioner caspase-3 and -7 in the mouse myocardium and studied its effects. Caspase knockout hearts are hypoplastic at birth, reaching normal weight progressively through myocyte hypertrophy. To identify the molecular pathways involved in these effects, we used microarray-based transcriptomics and multiplexed quantitative proteomics to compare wild type and executioner caspase-deficient myocardium at different developmental stages. Transcriptomics showed reduced expression of genes promoting DNA replication and cell cycle progression in the neonatal caspase-deficient heart suggesting reduced myocyte proliferation, and expression of non-cardiac isoforms of structural proteins in the adult null myocardium. Proteomics showed reduced abundance of proteins involved in oxidative phosphorylation accompanied by increased abundance of glycolytic enzymes underscoring retarded metabolic maturation of the caspase-null myocardium. Correlation between mRNA expression and protein abundance of relevant genes was confirmed, but transcriptomics and proteomics indentified complementary molecular pathways influenced by caspases in the developing heart. Forced expression of wild type or proteolytically inactive caspases in cultured cardiomyocytes induced expression of genes promoting cell division. The results reveal that executioner caspases can modulate heart's cellularity and maturation during development, contributing novel information about caspase biology and heart development.Executioner Caspase-3 and 7 Deficiency Reduces Myocyte Number in the Developing Mouse Heart.Cardona Maria M,López Juan Antonio JA,Serafín Anna A,Rongvaux Anthony A,Inserte Javier J,García-Dorado David D,Flavell Richard R,Llovera Marta M,Cañas Xavier X,Vázquez Jesús J,Sanchis Daniel D,Cardona Maria M, López Juan Antonio JA, Serafín Anna A, Rongvaux Anthony A, Inserte Javier J, García-Dorado David D, Flavell Richard R, Llovera Marta M, Cañas Xavier X, Vázquez Jesús J, Sanchis Daniel D3.4.22.-, vertebrate heart, dorsal tube, Herz, CPP-32, Entire heart, Laboratory, Caspase-3, Mus domesticus, number, mouse, branchial heart, adult heart, Yama, mini-ICE, CASP-14, extra or missing physical or functional parts, Hearts, Lice, presence, House Mouse, mice C57BL/6xCBA/CaJ hybrid, muscle cell, Mus muscaris, mereological quality, circulatory vessel, count in organism, chambered heart, muscle fiber, count, House, Mus, CASP-3, Mini-ICE, heart, Mus musculus domesticus, Apopain, cardium, Mice, cardiac structure., Mus musculus, mldy, Caspase-14 subunit p10, Swiss, heart or heart like organ, mice, Swiss Mouse, CC3, A830040C14Rik, House Mice, Swiss Mice, AC-3, Caspase-14 subunit p19, number of, "mouse" EXACT common_name [], MICE, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], SCA-1, cardiac pump, Herz@de, myocyte, has or lacks parts of type, cardinality, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, quantitative, house mouse, CPP32, Laboratory Mouse, presence or absence in organismprojections, Heart, 3.4.22.-, Tisp40beta, Herz, CPP-32, Entire heart, single-organism developmental process, AI461847, Laboratory, TISP40, lamellae, AP-1, Mus domesticus, jal, branchial heart, adult heart, mini-ICE, Atce1, CASP-14, process of organ, House Mouse, protrusion, Cardiac Muscle, lamella, circulatory vessel, Cardiac, Cardiac Muscles, House, CASP-3, Mus musculus domesticus, Apopain, Pgi, Menopausal hormone therapy, Gpi-1, Mice, Menopausal Hormone Therapy, Junc, ATCE1, mldy, Gpi-1r, Nlk, heart or heart like organ, Swiss, Gpi-1s, Phi, Gpi-1t, A830040C14Rik, Swiss Mice, AC-3, ridges, Myocardia, cardiac structure, mJAL, "mouse" EXACT common_name [], "Mus muscaris" RELATED misnomer [], cardiac pump, Muscles, papilla, accidental cell death, c-Jun, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], necrosis, house mouse, Gata-3, laminae, Gpi, vertebrate heart, dorsal tube, data, anatomical protrusion, HRT, Caspase-3, anatomical process, c-jun, lamina, mouse, Heart Muscle, flanges, Yama, Hearts, MF, Muscle, Amf, Lice, Cell, cardiac development, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, development, heart muscle, chambered heart, AV258827, heart myocardium, mOC-X, AIBZIP, Mus, heart, Mini-ICE, shelf, CREB3, muscle of heart, NK|GPI, cardium, 5330432F22Rik, Death, flange, NK, hJAL, organ process, CREB4, Mus musculus, Org, ORG, Caspase-14 subunit p10, mice, Hormone Replacement Therapy, shelves, Tisp40alpha, Swiss Mouse, CC3, Gpi1-r, Gpi1-s, House Mice, Caspase-14 subunit p19, Heart Muscles, Gpi1-t, MICE, projection, ridge, 1700012K17Rik, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], AP1, process, processes, SCA-1, Herz@de, cardiac muscle., spine, DEL, dorsal vessel development, processus, JAL, Mouse, CPP32, Entire myocardium, AV040530, Laboratory Mouse, Bglap-rs1, Gpi1s3.4.22.-, vertebrate heart, dorsal tube, Herz, CPP-32, Entire heart, Laboratory, Caspase-3, Mus domesticus, number, mouse, branchial heart, adult heart, Yama, mini-ICE, CASP-14, extra or missing physical or functional parts, Hearts, Lice, presence, House Mouse, mice C57BL/6xCBA/CaJ hybrid, muscle cell, Mus muscaris, mereological quality, circulatory vessel, count in organism, chambered heart, muscle fiber, count, House, Mus, CASP-3, Mini-ICE, heart, Mus musculus domesticus, Apopain, cardium, Mice, cardiac structure., Mus musculus, mldy, Caspase-14 subunit p10, Swiss, heart or heart like organ, mice, Swiss Mouse, CC3, A830040C14Rik, House Mice, Swiss Mice, AC-3, Caspase-14 subunit p19, number of, "mouse" EXACT common_name [], MICE, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], "Mus muscaris" RELATED misnomer [], SCA-1, cardiac pump, Herz@de, myocyte, has or lacks parts of type, cardinality, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Mouse, quantitative, house mouse, CPP32, Laboratory Mouse, presence or absence in organismprojections, Heart, Herz, Materials, CPP-32, single-organism developmental process, Laboratory, adult stage, Mus domesticus, A4, Phases, adult heart, CASP-14, House Mouse, Divisions, Cardiac Muscle, circulatory vessel, Cardiac Muscles, Oxidative, CASP-3, cardiac development., Autonomous Replication, present in fewer numbers in organism, adult, average, Cell Division Cycles, increased, mldy, M Phases, enzymes, heart or heart like organ, M, N, hypoplasia, Swiss Mice, proteins, Myocardia, cardiac structure, Autonomous, Division Phase, decreased, Muscles, myocyte proliferation, papilla, Cell Cycles, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], necrosis, house mouse, Childbirths, NEWBORN (0-27 DAYS), vertebrate heart, dorsal tube, anatomical protrusion, Caspase-3, lamina, mouse, flanges, Yama, Normalcy, Lice, Cell Divisions, results, mice C57BL/6xCBA/CaJ hybrid, Division Cycles, heart muscle, chambered heart, heart myocardium, count, weight, Mini-ICE, shelf, muscle of heart, Genetic Materials, cardium, Adults, Hypertrophies, Genetic Material, Mus musculus, Phosphorylation, adults, protein_coding_transcript, Caspase-14 subunit p10, Division Cycle, mice, shelves, Swiss Mouse, Caspase-14 subunit p19, Heart Muscles, MICE, projection, ridge, domesticus, Health, myocyte, DMDA, Replications, spine, Material, microarray, Autonomous Replications, Cistron, Mouse, DNA, CPP32, Entire myocardium, accessory, Childbirth, 3.4.22.-, Entire heart, lamellae, Biocatalysts, number, branchial heart, Parturitions, Gene, mini-ICE, process of organ, presence, supernumerary, TYPE, protrusion, DAGA4, lamella, Cardiac, Births, reduced, House, subnumerary, Cycle, Gene Products, Mus musculus domesticus, Apopain, tiny, MAM, Mice, SCG3, DNA Replications, Normalities, Cycles, Genetic, Cell Division Phase, Swiss, Birth, Cell Division Cycle, cell-division cycle, A830040C14Rik, messenger RNA, AC-3, Cell Division, ridges, decreased number, "mouse" EXACT common_name [], respiratory-chain phosphorylation, Division, "Mus muscaris" RELATED misnomer [], cardiac pump, template RNA, Oxidative Phosphorylations, accidental cell death, laminae, small, Neonatal, anatomical process, Proteins, Heart Muscle, Phosphorylations, enzyme activity, Hearts, Muscle, Cistrons, Cell, cardiac development, cardiac muscle, Mus muscaris, muscle cell, LGMD2C, development, polypeptide, count in organism, muscle fiber, Mus, heart, Protein, INSDC_feature:mRNA, Death, Replication, M Phase, flange, organ process, Phase, hypertrophy, DMDA1, underdeveloped, mRNA, Normality, increased number, CC3, House Mice, Laboratory Mice, Protein Gene Products, "house mouse" EXACT genbank_common_name [], process, processes, Gene Proteins, present in greater numbers in organism, SCA-1, Herz@de, DEL, SCARMD2, dorsal vessel development, processus, Newborn, quantitative, Laboratory Mouse, presence or absence in organism1203http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017677dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0varsite~00.00.00.0037014188772362740.001.0falseExecutioner Caspase-3 and 7 Deficiency Reduces Myocyte Number in the Developing Mouse Heart.Data from PeptideAtlas, [[http://www.peptideatlas.org/PASS/PASS00689 PASS00689]]. File: JAL_DanSanc_Hrt_iTRAQ_Fr6.mzml. Published as part of PLoS One. 2015 Jun 29;10(6):e0131411 . From the Abstract: {{i}} Executioner caspase-3 and -7 are proteases promoting cell death but non-apoptotic roles are being discovered. The heart expresses caspases only during development, suggesting they contribute to the organ maturation process. Therefore, we aimed at identifying novel functions of caspases in heart development. We induced simultaneous deletion of executioner caspase-3 and -7 in the mouse myocardium and studied its effects ... {{/i}}2015-08-16GPM3232001767726121671